MicroRNA-7 Specifically Marks the Gut Endocrine Lineage and Controls Progenitor Cell Proliferation Through Egfr
34 Pages Posted: 26 Sep 2018 Sneak Peek Status: Review CompleteMore...
Enteroendocrine cells (EECs) are critical for orchestrating proper responses to nutritional and microbial input. Importantly, EEC progenitors exhibit stem cell potential and contribute to intestinal epithelial proliferation; however, the molecular mechanisms are not well understood. In this study, we first report that miR-7 is a highly specific marker of the EEC lineage and is the most enriched miRNA in Prox1+ EEC progenitors. Next, we show that miR-7 is functionally suppressed in EEC progenitors by pro-proliferative perturbations in mouse and fruit fly. We then demonstrate that miR-7 exerts highly conserved control of intestinal epithelial proliferation and further show that miR-7 regulates the balance between proliferation and differentiation in murine EEC progenitors. Importantly, we also provide the mechanistic insight that miR-7 function is mediated by suppression of Egfr signaling. Overall, this study uncovers a novel role for miRNAs, specifically miR-7, in marking the EEC lineage and controlling the behavior of EEC progenitors.
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