Iron (II) and Other Heavy-Metal Tolerance in Bacteria Isolated From Rock Varnish in the Arid Region of Al-Jafer Basin, Jordan

Biodiversitas, Volume 18, Number 3, Pages: 1250-1257, E-ISSN: 2085-4722

8 Pages Posted: 21 Oct 2018

Date Written: July 29, 2017

Abstract

This study deals with enumeration, identification and molecular characterization of bacterial species tolerant of iron (II) and other heavy metals that isolated from rock varnish collected from the arid region of Al-Jafer Basin, Jordan. Based on conventional culture-dependent methods, six isolates exhibited a high degree of tolerance to iron (II) and other heavy metals with a minimum inhibitory concentration (550–600 mg/L) to iron, (500-550 mg/L) to lead, (350 mg/L) to copper, (400-450 mg/L) to chromium, (150-200 mg/L) to silver, and (150-200 mg/L) to mercury, in solid media. The results showed that all isolates could tolerate 2000 mg/L Fe 2 expressed as maximum tolerance concentration (MTC). The multiple metal resistances of these isolates were also associated with multiple antibiotic resistances. These iron tolerant isolates were shown to be capable of growth at temperatures ranging from 30 to 40°C and pH values ranging from 5 to 9 with some differences between the six isolates. On the basis of morphology and of 16S rDNA gene sequencing and phylogeny analysis, the iron-tolerant isolates were identified as Bacillus zhangzhouensis (DVCr1), Staphylococcus warneri (DVCr6), Bacillus cereus (DVCr7), Staphylococcus pasteuri (DVCu2), Brevibacterium frigoritolerans (DVPb) and Bacillus altitudinis (DVCr10). The 16S rDNA sequences from five of the six strains were submitted to GenBank and are available under the accession numbers KX781139, KX781140, KX781141, KX781145 and KX781148. The results highlight the high potential of a non-polluted habitat like rock varnish to provide new microbial communities that could be used for enhanced bioremediation of heavy metals from a contaminated site.

Keywords: Al-Jafer Basin, Rock Varnish, Bacteria Tolerant of Heavy Metals, 16S rDNA

JEL Classification: Q57

Suggested Citation

Alnaimat, Sulaiman, Iron (II) and Other Heavy-Metal Tolerance in Bacteria Isolated From Rock Varnish in the Arid Region of Al-Jafer Basin, Jordan (July 29, 2017). Biodiversitas, Volume 18, Number 3, Pages: 1250-1257, E-ISSN: 2085-4722, Available at SSRN: https://ssrn.com/abstract=3256646

Sulaiman Alnaimat (Contact Author)

Al-Hussein Bin Talal University ( email )

P.O. Box 20
Ma'an
Jordan
00962772229392 (Phone)

HOME PAGE: http://www.ahu.edu.jo

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