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A Cellular-Resolution Atlas of the Larval Zebrafish Brain

58 Pages Posted: 1 Oct 2018 Sneak Peek Status: Under Review

See all articles by Michael Kunst

Michael Kunst

Max Planck Institute of Neurobiology - Department Genes – Circuits – Behavior

Eva Laurell

Max Planck Institute of Neurobiology - Department Genes – Circuits – Behavior

Nouwar Mokayes

Max Planck Institute of Neurobiology - Department Genes – Circuits – Behavior

Anna Kramer

Max Planck Institute of Neurobiology - Department Genes – Circuits – Behavior

Fumi Kubo

Max Planck Institute of Neurobiology - Department Genes – Circuits – Behavior

Antonio M. Fernandes

Max Planck Institute of Neurobiology - Department Genes – Circuits – Behavior

Dominique Förster

Max Planck Institute of Neurobiology - Department Genes – Circuits – Behavior

Marco Dal Maschio

Max Planck Institute of Neurobiology - Department Genes – Circuits – Behavior

Herwig Baier

Max Planck Institute of Neurobiology - Department Genes – Circuits – Behavior

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Abstract

Understanding brain-wide neuronal dynamics requires a detailed map of the underlying circuit architecture. We built an interactive cellular-resolution atlas of the zebrafish brain at six days post-fertilization (6 dpf) based on the reconstructions of over 2,000 individually GFP-labeled neurons. We clustered our dataset in 'cell types', establishing a unique database of quantitatively described neuronal morphologies together with their spatial coordinates in vivo. Over a hundred transgene expression patterns (mainly Gal4 lines) were imaged separately and co-registered with the single-neuron atlas. By annotating 73 non-overlapping brain regions, we generated from our dataset an inter-areal wiring diagram of the larval brain, which serves as ground truth for synapse-scale, electron microscopic reconstructions. Graph theoretical analysis identified statistical properties of this network, including hierarchically organized modules interlinked by central information hubs. Interrogating our atlas by 'virtual tract tracing' has already revealed previously unknown topographical relationships among sensory afferents in the tectum and between the cerebellum and its multiple projection targets. In conclusion, we present here an evolving computational resource and visualization tool, which will be essential to map function to structure in a vertebrate brain.

Suggested Citation

Kunst, Michael and Laurell, Eva and Mokayes, Nouwar and Kramer, Anna and Kubo, Fumi and Fernandes, Antonio M. and Förster, Dominique and Dal Maschio, Marco and Baier, Herwig, A Cellular-Resolution Atlas of the Larval Zebrafish Brain (September 29, 2018). Available at SSRN: https://ssrn.com/abstract=3257346 or http://dx.doi.org/10.2139/ssrn.3257346
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Michael Kunst

Max Planck Institute of Neurobiology - Department Genes – Circuits – Behavior

Am Klopferspitz 18
Planegg, 82152
Germany

Eva Laurell

Max Planck Institute of Neurobiology - Department Genes – Circuits – Behavior

Am Klopferspitz 18
Planegg, 82152
Germany

Nouwar Mokayes

Max Planck Institute of Neurobiology - Department Genes – Circuits – Behavior

Am Klopferspitz 18
Planegg, 82152
Germany

Anna Kramer

Max Planck Institute of Neurobiology - Department Genes – Circuits – Behavior

Am Klopferspitz 18
Planegg, 82152
Germany

Fumi Kubo

Max Planck Institute of Neurobiology - Department Genes – Circuits – Behavior

Am Klopferspitz 18
Planegg, 82152
Germany

Antonio M. Fernandes

Max Planck Institute of Neurobiology - Department Genes – Circuits – Behavior

Am Klopferspitz 18
Planegg, 82152
Germany

Dominique Förster

Max Planck Institute of Neurobiology - Department Genes – Circuits – Behavior

Am Klopferspitz 18
Planegg, 82152
Germany

Marco Dal Maschio

Max Planck Institute of Neurobiology - Department Genes – Circuits – Behavior

Am Klopferspitz 18
Planegg, 82152
Germany

Herwig Baier (Contact Author)

Max Planck Institute of Neurobiology - Department Genes – Circuits – Behavior ( email )

Am Klopferspitz 18
Planegg, 82152
Germany

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