Profile of Thin-Layer Chromatography and UV-Vis Spectrophotometry of Akar Kuning Stem Extract (Arcangelisia flava)

Borneo Journal of Pharmacy (2018)

5 Pages Posted: 31 Oct 2018

Date Written: November 1, 2018

Abstract

This study aims to obtain the profile of Thin-Layer Chromatography (TLC) and Ultraviolet-Visible (UV-Vis) spectrophotometry from ethanol extract of akar kuning stems (Arcangelisia flava) from Central Kalimantan. The TLC method is used with the orientation phase of the combination of polar-non-polar solvents resulting from orientation, while ethanol is used as the solvent for UV-Vis spectrophotometers. TLC results showed the formation of 3 stains on a combination of polar solvents chloroform : methanol : water while in a non-polar solvent combination n-hexane : ethyl acetate did not show any stains. Comparison of retention factor (Rf) values shows the best combination of polar solvents to separate stains at a ratio of 5 : 2 : 1, respectively. Separation in 2-dimensional TLC with polar solvents showed a similar pattern with 1-dimensional separation in the form of 3 stains. UV-Vis spectrophotometer results showed 4 main peaks with wavelength 227.2; 267.4; 345.2; and 425.3 nm, respectively. The profile of the peak formed is very similar to that shown by berberine, one of the main metabolites of akar kuning. TLC and UV-Vis spectrophotometers profiles obtained are expected to support further research using akar kuning stems, especially those from Central Kalimantan.

Keywords: Akar kuning, TLC, UV-Vis Spectrophotometry

Suggested Citation

Pratama, Mohammad Rizki Fadhil and Suratno, Suratno and Mulyani, Evi, Profile of Thin-Layer Chromatography and UV-Vis Spectrophotometry of Akar Kuning Stem Extract (Arcangelisia flava) (November 1, 2018). Borneo Journal of Pharmacy (2018). Available at SSRN: https://ssrn.com/abstract=3263333

Mohammad Rizki Fadhil Pratama (Contact Author)

Universitas Muhammadiyah Palangkaraya ( email )

RTA Milono Street Km. 1.5
Palangka Raya, Central Kalimantan 73111
Indonesia

HOME PAGE: http://www.umpalangkaraya.ac.id

Suratno Suratno

Suratno ( email )

RTA Milono Street Km. 1.5
Palangka Raya, Central Borneo 73111
Indonesia

Evi Mulyani

Evi Mulyani ( email )

RTA Milono Street Km. 1.5
Palangka Raya, Central Borneo 73111
Indonesia

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