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Transcriptome Sequencing of Adenomyosis Eutopic Endometrium: A New Insight into Its Pathophysiology

45 Pages Posted: 25 Oct 2018

See all articles by Yuqian Xiang

Yuqian Xiang

Shanghai Jiao Tong University (SJTU) - International Peace Maternity and Child Health Hospital

Yabing Sun

Shanghai Jiao Tong University (SJTU) - International Peace Maternity and Child Health Hospital

Bingxin Yang

Shanghai Jiao Tong University (SJTU) - International Peace Maternity and Child Health Hospital

Yeping Yang

Shanghai Jiao Tong University (SJTU) - International Peace Maternity and Child Health Hospital

Ying Zhang

Shanghai Jiao Tong University (SJTU) - International Peace Maternity and Child Health Hospital

Tiantian Yu

Shanghai Jiao Tong University (SJTU) - International Peace Maternity and Child Health Hospital

Hefeng Huang

Zhejiang University - Key Laboratory of Reproductive Genetics (Ministry of Education); Zhejiang University - Department of Reproductive Endocrinology

Junyu Zhang

Shanghai Jiao Tong University (SJTU) - International Peace Maternity and Child Health Hospital

Hong Xu

Shanghai Jiao Tong University (SJTU) - International Peace Maternity and Child Health Hospital

More...

Abstract

Background: The eutopic endometrium has been suggested to play a crucial role in the pathogenesis of adenomyosis. However, the specific genes in eutopic endometrium responsible for the pathogenesis of adenomyosis still remain to be elucidated. We aim to identify differentially expressed genes (DEGs) and molecular pathways/networks in eutopic endometrium from adenomyosis patients, and provide a new insight into disease mechanisms at a transcriptome level.

Methods: RNA sequencing (RNA-Seq) was performed with 12 eutopic endometrium from adenomyosis and control groups. DEGs in adenomyosis were validated by quantitative real-time PCR (qPCR) and immunochemistry. Functional annotations of the DEGs were analyzed with Ingenuity Pathway Analysis (IPA). Quantitative DNA methylation analysis of CEBPB was performed with MassArray system.

Findings: Totally, 373 genes were differentially expressed in the adenomyosis eutopic endometrium compared to matched controls. Bioinformatic analysis indicated that IL-6 signaling and ERK/MAPK signaling was activated in adenomyosis endometrium. We also found that the increased expression and DNA hypomethylation of C/EBPβ were associated with adenomyosis.

Interpretation: Our results revealed key pathways and networks in eutopic endometrium of adenomyosis. The study is the first to propose the link between C/EBPβ and adenomyosis and can improve the understanding of the pathogenesis of adenomyosis.

Funding Statement: This work is supported by the National Natural Science Foundation of China [NO. 81771551, 81501276 and 81401219], the Shanghai Municipal Commission of Science and Technology Program [NO. 17411972800 and 15411966700], the Shanghai Municipal Commission of Health and Family Planning [NO. 20154Y0039], the Municipal Human Resources Development Program for Outstanding Young Talents in Medical and Health Sciences in Shanghai [NO. 2018YQ39], the Shanghai Shenkang Hospital Development Center Clinical Science and Technology Innovation Project [NO. SHDC12017123], and the Combined Engineering and Medical Project of Shanghai Jiao Tong University [NO. YG2015MS41 and YG2017MS39].

Declaration of Interests: The authors have declared that no competing interests exist.

Ethics Approval Statement: The study protocols were approved by the Ethics Review Committee of IPMCH and conducted according to the Declaration of Helsinki Principles.

Keywords: Adenomyosis, Eutopic endometrium, RNA sequencing, C/EBPβ, DNA methylation

Suggested Citation

Xiang, Yuqian and Sun, Yabing and Yang, Bingxin and Yang, Yeping and Zhang, Ying and Yu, Tiantian and Huang, Hefeng and Zhang, Junyu and Xu, Hong, Transcriptome Sequencing of Adenomyosis Eutopic Endometrium: A New Insight into Its Pathophysiology (October 17, 2018). Available at SSRN: https://ssrn.com/abstract=3269588 or http://dx.doi.org/10.2139/ssrn.3269588

Yuqian Xiang (Contact Author)

Shanghai Jiao Tong University (SJTU) - International Peace Maternity and Child Health Hospital ( email )

1961 Huashan Rd
Shanghai Shi, 200030
China

Yabing Sun

Shanghai Jiao Tong University (SJTU) - International Peace Maternity and Child Health Hospital

1961 Huashan Rd
Shanghai Shi, 200030
China

Bingxin Yang

Shanghai Jiao Tong University (SJTU) - International Peace Maternity and Child Health Hospital

1961 Huashan Rd
Shanghai Shi, 200030
China

Yeping Yang

Shanghai Jiao Tong University (SJTU) - International Peace Maternity and Child Health Hospital

1961 Huashan Rd
Shanghai Shi, 200030
China

Ying Zhang

Shanghai Jiao Tong University (SJTU) - International Peace Maternity and Child Health Hospital

1961 Huashan Rd
Shanghai Shi, 200030
China

Tiantian Yu

Shanghai Jiao Tong University (SJTU) - International Peace Maternity and Child Health Hospital

1961 Huashan Rd
Shanghai Shi, 200030
China

Hefeng Huang

Zhejiang University - Key Laboratory of Reproductive Genetics (Ministry of Education) ( email )

Zhejiang, 310058
China

Zhejiang University - Department of Reproductive Endocrinology

Zhejiang
China

Junyu Zhang

Shanghai Jiao Tong University (SJTU) - International Peace Maternity and Child Health Hospital ( email )

1961 Huashan Rd
Shanghai Shi, 200030
China

Hong Xu

Shanghai Jiao Tong University (SJTU) - International Peace Maternity and Child Health Hospital ( email )

1961 Huashan Rd
Shanghai Shi, 200030
China

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