puc-header

A Retino-Retinal Connection Guided by Unc5c Emerged in Species with Retinal Waves

48 Pages Posted: 24 Oct 2018 Sneak Peek Status: Review Complete

See all articles by Verónica Murcia-Belmonte

Verónica Murcia-Belmonte

Miguel Hernandez University - Institute of Neurosciences

Celia Vegar

Miguel Hernandez University - Institute of Neurosciences

Yaiza Coca

Miguel Hernandez University - Institute of Neurosciences

Camino de Juan

Miguel Hernandez University - Institute of Neurosciences

Arturo José Valiño

Miguel Hernandez University - Institute of Neurosciences

Salvador Sala

Miguel Hernandez University - Institute of Neurosciences

Ronan DaSilva

Institut de recherches cliniques de Montréal (IRCM)

Santiago Negueruela

Miguel Hernandez University - Institute of Neurosciences

Artur Kania

Institut de recherches cliniques de Montréal (IRCM)

Víctor Borrell

Miguel Hernandez University - Institute of Neurosciences

Luis M. Martinez

Miguel Hernandez University - Institute of Neurosciences

Lynda Erskine

University of Aberdeen - ​Institute​ ​of​ ​Medical​ ​Sciences

Eloísa Herrera

Miguel Hernandez University - Institute of Neurosciences

More...

Abstract

The existence of axons extending from one retina to the other has been reported during perinatal development in different vertebrates. However, it has been thought that these axons are either a labelling artefact or misprojections. Here, we unequivocally show that a small subset of retinal ganglion cells (RGCs) project to the opposite retina and that the guidance receptor Unc5c, expressed in the retinal region where the R-R neurons are located, is necessary and sufficient to guide axons to the opposite retina. In addition, Netrin1, an Unc5c ligand, is expressed in the ventral diencephalon in a pattern that is consistent with impeding the growth of Unc5c retinal axons into the brain. We have also generated a mathematical model to explore the formation of retinotopic maps in the presence and absence of a functional connection between both eyes. This model predicts that an R-R connection is required for the bilateral coordination of axonal refinement in species where refinement depends upon spontaneous retinal waves. Consistent with this idea, the retinal expression of Unc5c correlates with the existence and size of an R-R projection in different species and with the extent of axonal refinement in visual targets. These findings demonstrate that active guidance drives the formation of the R-R projection and suggest an important role for these projections in visual mapping to ensure congruent bilateral refinement.

Keywords: retino-retinal connectivity, RGCs, axon guidance, Unc5c, Netrin1, visual pathway, visual axon refinement

Suggested Citation

Murcia-Belmonte, Verónica and Vegar, Celia and Coca, Yaiza and de Juan, Camino and Valiño, Arturo José and Sala, Salvador and DaSilva, Ronan and Negueruela, Santiago and Kania, Artur and Borrell, Víctor and Martinez, Luis M. and Erskine, Lynda and Herrera, Eloísa, A Retino-Retinal Connection Guided by Unc5c Emerged in Species with Retinal Waves (October 24, 2018). Available at SSRN: https://ssrn.com/abstract=3272236 or http://dx.doi.org/10.2139/ssrn.3272236
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Verónica Murcia-Belmonte

Miguel Hernandez University - Institute of Neurosciences

Avenida de la universidad d'Elx, s/n
Elche, 03202
Spain

Celia Vegar

Miguel Hernandez University - Institute of Neurosciences

Avenida de la universidad d'Elx, s/n
Elche, 03202
Spain

Yaiza Coca

Miguel Hernandez University - Institute of Neurosciences

Avenida de la universidad d'Elx, s/n
Elche, 03202
Spain

Camino De Juan

Miguel Hernandez University - Institute of Neurosciences

Avenida de la universidad d'Elx, s/n
Elche, 03202
Spain

Arturo José Valiño

Miguel Hernandez University - Institute of Neurosciences

Avenida de la universidad d'Elx, s/n
Elche, 03202
Spain

Salvador Sala

Miguel Hernandez University - Institute of Neurosciences

Avenida de la universidad d'Elx, s/n
Elche, 03202
Spain

Ronan DaSilva

Institut de recherches cliniques de Montréal (IRCM)

110 avenue des Pins Ouest
Montreal, Quebec H2W 1R7
Canada

Santiago Negueruela

Miguel Hernandez University - Institute of Neurosciences

Avenida de la universidad d'Elx, s/n
Elche, 03202
Spain

Artur Kania

Institut de recherches cliniques de Montréal (IRCM)

110 avenue des Pins Ouest
Montreal, Quebec H2W 1R7
Canada

Víctor Borrell

Miguel Hernandez University - Institute of Neurosciences

Avenida de la universidad d'Elx, s/n
Elche, 03202
Spain

Luis M. Martinez

Miguel Hernandez University - Institute of Neurosciences

Avenida de la universidad d'Elx, s/n
Elche, 03202
Spain

Lynda Erskine

University of Aberdeen - ​Institute​ ​of​ ​Medical​ ​Sciences

​IMS​ ​Building,​ ​Foresterhill
Aberdeen, Scotland AB25​ ​2ZD
United Kingdom

Eloísa Herrera (Contact Author)

Miguel Hernandez University - Institute of Neurosciences ( email )

Avenida de la universidad d'Elx, s/n
Elche, 03202
Spain

Click here to go to Cell.com

Go to Cell.com

Paper statistics

Abstract Views
230
Downloads
7