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Imaging Molecular Transport Through the Membrane of a Living Cell

14 Pages Posted: 2 Nov 2018 Sneak Peek Status: Review Complete

See all articles by Mohammad Sharifian Gh.

Mohammad Sharifian Gh.

Temple University - Department of Chemistry

Michael Wilhelm

Temple University - Department of Chemistry

Michael Moore

Delaware State University - Optical Science Center for Applied Research

Hai-Lung Dai

Temple University - Department of Chemistry

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Abstract

We demonstrate that time-resolved second-harmonic (SH) light scattering, when applied as an imaging modality, can be used to spatially resolve the adsorption and transport rates of molecules diffusing across the membrane in a living cell. As a representative example, we measure the passive transport of the small amphiphilic ion, malachite green, across the plasma membrane in living human dermal fibroblast cells. Analysis of the time-resolved SH images reveals that membrane regions enduring higher stress exhibit slower transport rates. It is proposed that this stress-transport relation is a result of local enrichment of membrane rigidifiers as part of the cell response to maintain membrane integrity under high strain.

Suggested Citation

Sharifian Gh., Mohammad and Wilhelm, Michael and Moore, Michael and Dai, Hai-Lung, Imaging Molecular Transport Through the Membrane of a Living Cell (October 30, 2018). Available at SSRN: https://ssrn.com/abstract=3275294 or http://dx.doi.org/10.2139/ssrn.3275294
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Mohammad Sharifian Gh.

Temple University - Department of Chemistry

Philadelphia, PA 19122
United States

Michael Wilhelm (Contact Author)

Temple University - Department of Chemistry ( email )

Philadelphia, PA 19122
United States

Michael Moore

Delaware State University - Optical Science Center for Applied Research

Dover, DE 19901
United States

Hai-Lung Dai

Temple University - Department of Chemistry

Philadelphia, PA 19122
United States

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