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ZRANB2/SNHG20/FOXK1 Axis Regulates Vasculogenic Mimicry Formation in Glioma
31 Pages Posted: 10 Nov 2018More...
Background: Glioma is the most common intracranial neoplasm with vasculogenic mimicry formation as one form of blood supply. Many RNA-binding proteins and long non-coding RNAs are involved in tumorigenesis of glioma.
Methods: The expression of ZRANB2, SNHG20 and FOXK1 in glioma was detected by real-time PCR or western blot. The function of ZRANB2/SNHG20/FOXK1 axis in glioma associated with vasculogenic mimicry formation was analyzed.
Results: ZRANB2 was up-regulated in glioma tissues and glioma cells. ZRANB2 knockdown inhibited the proliferation, migration, invasion and vasculogenic mimicry formation of glioma cells. ZRANB2 bound to SNHG20 and increased its stability. Knockdown of SNHG20 reduced the degradation of FOXK1 mRNA by SMD pathway. FOXK1 inhibited transcription by binding to the promoters of MMP1, MMP9 and VE-Cadherin and inhibited vasculogenic mimicry formation of glioma cells.
Conclusions: ZRANB2/SNHG20/FOXK1 axis plays an important role in regulating vasculogenic mimicry formation of glioma, which might provide new targets of glioma therapy.
Funding Statement: This work is supported by grants from the Natural Science Foundation of China (81872073, 81872503, 81672511 and 81573010), Liaoning Science and Technology Plan Project (No.2017225020, 2015225007), Project of Key Laboratory of Neuro-oncology in Liaoning Province (112-2400017005), special developmental project guided by central government of Liaoning Province (No. 2017011553-301) and outstanding scientific fund of Shengjing hospital (No. 201304).
Declaration of Interests: The authors declare that they have no conflict of interest.
Ethics Approval Statement: All the experiments with mice were conducted strickly in accordance with the Animal Welfare Act approved by the Ethics Committee of China Medical University.
Keywords: ZRANB2, SNHG20, FOXK1, Glioma, Vasculogenic mimicry formation
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