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ZRANB2/SNHG20/FOXK1 Axis Regulates Vasculogenic Mimicry Formation in Glioma

31 Pages Posted: 10 Nov 2018

See all articles by Xiaozhi Li

Xiaozhi Li

China Medical University - Department of Stomatology

Yixue Xue

China Medical University - Department of Neurobiology; China Medical University - Key Laboratory of Cell Biology; China Medical University - Key Laboratory of Medical Cell Biology

Xiaobai Liu

China Medical University - Department of Neurosurgery; China Medical University - Provincial Research Center of Translational Medicine in Nervous System Diseases; Key Laboratory of Neuro-oncology in Liaoning Province; Liaoning Clinical Medical Research Center in Nervous System Disease

Jian Zheng

China Medical University - Department of Neurosurgery; China Medical University - Provincial Research Center of Translational Medicine in Nervous System Diseases; Key Laboratory of Neuro-oncology in Liaoning Province; Liaoning Clinical Medical Research Center in Nervous System Disease

Shuyuan Shen

China Medical University - Department of Neurobiology; China Medical University - Key Laboratory of Cell Biology; China Medical University - Key Laboratory of Medical Cell Biology

Chunqing Yang

China Medical University - Department of Neurosurgery; China Medical University - Provincial Research Center of Translational Medicine in Nervous System Diseases; Key Laboratory of Neuro-oncology in Liaoning Province

Jiajian Chen

Southwestern University of Finance and Economics (SWUFE)

Zhen Li

China Medical University - Shengjing Hospital

Liubo Liu

China Medical University

Jun Ma

China Medical University

Teng Ma

China Medical University

Yunhui Liu

China Medical University - Department of Neurosurgery; China Medical University - Provincial Research Center of Translational Medicine in Nervous System Diseases; Key Laboratory of Neuro-oncology in Liaoning Province; Liaoning Clinical Medical Research Center in Nervous System Disease

More...

Abstract

Background: Glioma is the most common intracranial neoplasm with vasculogenic mimicry formation as one form of blood supply. Many RNA-binding proteins and long non-coding RNAs are involved in tumorigenesis of glioma.

Methods: The expression of ZRANB2, SNHG20 and FOXK1 in glioma was detected by real-time PCR or western blot. The function of ZRANB2/SNHG20/FOXK1 axis in glioma associated with vasculogenic mimicry formation was analyzed.

Results: ZRANB2 was up-regulated in glioma tissues and glioma cells. ZRANB2 knockdown inhibited the proliferation, migration, invasion and vasculogenic mimicry formation of glioma cells. ZRANB2 bound to SNHG20 and increased its stability. Knockdown of SNHG20 reduced the degradation of FOXK1 mRNA by SMD pathway. FOXK1 inhibited transcription by binding to the promoters of MMP1, MMP9 and VE-Cadherin and inhibited vasculogenic mimicry formation of glioma cells.

Conclusions: ZRANB2/SNHG20/FOXK1 axis plays an important role in regulating vasculogenic mimicry formation of glioma, which might provide new targets of glioma therapy.

Funding Statement: This work is supported by grants from the Natural Science Foundation of China (81872073, 81872503, 81672511 and 81573010), Liaoning Science and Technology Plan Project (No.2017225020, 2015225007), Project of Key Laboratory of Neuro-oncology in Liaoning Province (112-2400017005), special developmental project guided by central government of Liaoning Province (No. 2017011553-301) and outstanding scientific fund of Shengjing hospital (No. 201304).

Declaration of Interests: The authors declare that they have no conflict of interest.

Ethics Approval Statement: All the experiments with mice were conducted strickly in accordance with the Animal Welfare Act approved by the Ethics Committee of China Medical University.

Keywords: ZRANB2, SNHG20, FOXK1, Glioma, Vasculogenic mimicry formation

Suggested Citation

Li, Xiaozhi and Xue, Yixue and Liu, Xiaobai and Zheng, Jian and Shen, Shuyuan and Yang, Chunqing and Chen, Jiajian and Li, Zhen and Liu, Liubo and Ma, Jun and Ma, Teng and Liu, Yunhui, ZRANB2/SNHG20/FOXK1 Axis Regulates Vasculogenic Mimicry Formation in Glioma (June 11, 2018). Available at SSRN: https://ssrn.com/abstract=3279904 or http://dx.doi.org/10.2139/ssrn.3279904

Xiaozhi Li

China Medical University - Department of Stomatology

No. 91 Hsueh-Shih Road
Taichung, 40402
Taiwan

Yixue Xue

China Medical University - Department of Neurobiology ( email )

Shenyang, 110122
China

China Medical University - Key Laboratory of Cell Biology ( email )

Shenyang, 110122
China

China Medical University - Key Laboratory of Medical Cell Biology ( email )

Shenyang, 110122
China

Xiaobai Liu

China Medical University - Department of Neurosurgery

Shenyang, 110004
China

China Medical University - Provincial Research Center of Translational Medicine in Nervous System Diseases

Shenyang, 110004
China

Key Laboratory of Neuro-oncology in Liaoning Province

Shenyang, 110004
China

Liaoning Clinical Medical Research Center in Nervous System Disease

China

Jian Zheng

China Medical University - Department of Neurosurgery

Shenyang, 110004
China

China Medical University - Provincial Research Center of Translational Medicine in Nervous System Diseases

Shenyang, 110004
China

Key Laboratory of Neuro-oncology in Liaoning Province

Shenyang, 110004
China

Liaoning Clinical Medical Research Center in Nervous System Disease

China

Shuyuan Shen

China Medical University - Department of Neurobiology

Shenyang, 110122
China

China Medical University - Key Laboratory of Cell Biology

Shenyang, 110122
China

China Medical University - Key Laboratory of Medical Cell Biology

Shenyang, 110122
China

Chunqing Yang

China Medical University - Department of Neurosurgery

Shenyang, 110004
China

China Medical University - Provincial Research Center of Translational Medicine in Nervous System Diseases

Shenyang, 110004
China

Key Laboratory of Neuro-oncology in Liaoning Province

Shenyang, 110004
China

Jiajian Chen

Southwestern University of Finance and Economics (SWUFE) ( email )

Zhizhi Zone 236, 555 Liutai Avenue
Wenjiang District
Chengdu, Sichuan 611130
China

Zhen Li

China Medical University - Shengjing Hospital

Shenyang, Liaoning 110004
China

Liubo Liu

China Medical University

No. 91 Hsueh-Shih Road
Taichung, 40402
Taiwan

Jun Ma

China Medical University

No. 91 Hsueh-Shih Road
Taichung, 40402
Taiwan

Teng Ma

China Medical University

No. 91 Hsueh-Shih Road
Taichung, 40402
Taiwan

Yunhui Liu (Contact Author)

China Medical University - Department of Neurosurgery

Shenyang, 110004
China

China Medical University - Provincial Research Center of Translational Medicine in Nervous System Diseases

Shenyang, 110004
China

Key Laboratory of Neuro-oncology in Liaoning Province

Shenyang, 110004
China

Liaoning Clinical Medical Research Center in Nervous System Disease

China

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