Aedes Aegypti Mosquitoes Detect Acidic Volatiles in Human Odor Using the IR8a Pathway
60 Pages Posted: 9 Nov 2018 Sneak Peek Status: Under ReviewMore...
Mosquitoes use olfaction as a primary means of detecting their hosts. Previously, the genetic ablation of a family of Aedes aegypti olfactory receptors, the Odorant Receptors (ORs), was not sufficient to reduce host-seeking in the presence of carbon dioxide (CO2). This suggests the olfactory receptors that remain, such as the Ionotropic Receptors (IRs), could play a significant role in host detection. To test this, we disrupted the Ir8a co-receptor in Ae. aegypti using CRISPR/Cas9. Ir8a mutant female mosquitoes are not attracted to lactic acid, a behaviorally active component of human odor, and lack odor-evoked responses to acidic volatiles. The loss of Ir8a reduces mosquito attraction to humans and their odor. We show that the CO2-detection pathway is necessary but not sufficient for IR8a to detect human odor. Our study reveals that the IR8a pathway is crucial for an anthropophilic vector mosquito to effectively seek hosts.
Keywords: Mosquito, olfaction, Ionotropic Receptors, IR8a, human sweat, lactic acid, Aedes aegypti, genetics, host-seeking, blood-feeding, vector biology
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