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Overexpression of Human NPY or AgRP in the Paraventricular Nucleus Increase Bodyweight in Young Rhesus Macaques

20 Pages Posted: 14 Nov 2018 Sneak Peek Status: Under Review

See all articles by Tao Zhou

Tao Zhou

ShanghaiTech University - School of Life Science and Technology

Ke Tang

Guangzhou University, School of Life Sciences, Precise Genome Engineering Center

Zhengwu Wang

Sichuan Hengshu Bio-Technology Co., Ltd.

Zunfu Ke

Sun Yat-Sen University (SYSU), Zhongshan School of Medicine, First Affiliated Hospital, Department of Pathology; Sun Yat-Sen University (SYSU) - Institute of Precision Medicine

Fan Huang

Government of the People's Republic of China, People's Liberation Army (PLA), First People’s Hospital of Yibin

Ruizhi Tan

Southwest Medical University, Affiliated Traditional Medicine Hospital, Research Center of Integrated Chinese and Western Medicine

Xu Luo

Sichuan Hengshu Bio-Technology Co., Ltd.

Xujiao Ling

Sichuan Hengshu Bio-Technology Co., Ltd.

Xuliang Zhang

ShanghaiTech University - School of Life Science and Technology

Li Wang

ShanghaiTech University - School of Life Science and Technology

Xudong Zhao

Chinese Academy of Sciences (CAS), Kunming Institute of Zoology, Kunming Primate Research Center

Hongli Li

Shanghai University of Traditional Chinese Medicine - Drug Discovery Lab

Shengjie Fan

Shanghai University of Traditional Chinese Medicine - Drug Discovery Lab

Shubin Hong

Sun Yat-Sen University (SYSU) - Institute of Precision Medicine

Sui Peng

Jining Medical University - Institute of Precision Medicine

Qingchun Tong

University of Texas at Houston, McGovern Medical School, Graduate School of Biological Sciences, Brown Foundation Institute of Molecular Medicine and Program in Neuroscience

Xingxu Huang

ShanghaiTech University - School of Life Science and Technology

Junming Fan

ShanghaiTech University - School of Life Science and Technology

Haipeng Xiao

Sun Yat-Sen University (SYSU) - Institute of Precision Medicine

Cheng Huang

Shanghai University of Traditional Chinese Medicine - Drug Discovery Lab

More...

Abstract

The role of neuropeptides agouti-related peptide (AgRP) and neuropeptide Y (NPY) in feeding behavior regulation have been extensively studied in rodents. However, the biological functions of these peptides in primates are not fully addressed. Here we shown that, through Lenti-virus mediated selective expression of human NPY (hNPY) or human AgRP (hAgRP) in the paraventricular nucleus (PVN), the bodyweight was significantly increased in young Rhesus Macaques. Whereas, the blood level of glucose and insulin, as well as metabolic parameters, including blood level of triglyceride (TG), total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL), non-esterified free fatty acids (NEFA) and leptin remain unchanged after virus injection. Although further investigations of long-term effects are needed, these results have recapitulated some parts of previous reports in rodents, and suggest that these non-germlines geneticlly modified monkeys may provide new models for studying feeding behaviors and related disease, such as obesity and diabetes.

Suggested Citation

Zhou, Tao and Tang, Ke and Wang, Zhengwu and Ke, Zunfu and Huang, Fan and Tan, Ruizhi and Luo, Xu and Ling, Xujiao and Zhang, Xuliang and Wang, Li and Zhao, Xudong and Li, Hongli and Fan, Shengjie and Hong, Shubin and Peng, Sui and Tong, Qingchun and Huang, Xingxu and Fan, Junming and Xiao, Haipeng and Huang, Cheng, Overexpression of Human NPY or AgRP in the Paraventricular Nucleus Increase Bodyweight in Young Rhesus Macaques (November 13, 2018). Available at SSRN: https://ssrn.com/abstract=3283709 or http://dx.doi.org/10.2139/ssrn.3283709
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Tao Zhou

ShanghaiTech University - School of Life Science and Technology

100 Haike Road, Zhangjiang Hi-Tech Park, Pudong
Research Building
Shanghai, 201210
China

Ke Tang

Guangzhou University, School of Life Sciences, Precise Genome Engineering Center

Waihuanxi Road 230
Guangzhou
China

Zhengwu Wang

Sichuan Hengshu Bio-Technology Co., Ltd.

Yibin 644000
Sichuan
China

Zunfu Ke

Sun Yat-Sen University (SYSU), Zhongshan School of Medicine, First Affiliated Hospital, Department of Pathology ( email )

Guangzhou, Guangdong
China

Sun Yat-Sen University (SYSU) - Institute of Precision Medicine ( email )

Guangzhou, Guangdong
China

Fan Huang

Government of the People's Republic of China, People's Liberation Army (PLA), First People’s Hospital of Yibin

Yibin 64400
Sichuan
China

Ruizhi Tan

Southwest Medical University, Affiliated Traditional Medicine Hospital, Research Center of Integrated Chinese and Western Medicine

Luzhou 646000
Sichuan
China

Xu Luo

Sichuan Hengshu Bio-Technology Co., Ltd.

Yibin 644000
Sichuan
China

Xujiao Ling

Sichuan Hengshu Bio-Technology Co., Ltd.

Yibin 644000
Sichuan
China

Xuliang Zhang

ShanghaiTech University - School of Life Science and Technology

100 Haike Road, Zhangjiang Hi-Tech Park, Pudong
Research Building
Shanghai, 201210
China

Li Wang

ShanghaiTech University - School of Life Science and Technology

100 Haike Road, Zhangjiang Hi-Tech Park, Pudong
Research Building
Shanghai, 201210
China

Xudong Zhao

Chinese Academy of Sciences (CAS), Kunming Institute of Zoology, Kunming Primate Research Center

No.32 Jiaochang Donglu
Kunming, Yunnan 650223
China

Hongli Li

Shanghai University of Traditional Chinese Medicine - Drug Discovery Lab

Shanghai, 201210
China

Shengjie Fan

Shanghai University of Traditional Chinese Medicine - Drug Discovery Lab

Shanghai, 201210
China

Shubin Hong

Sun Yat-Sen University (SYSU) - Institute of Precision Medicine

Guangzhou, Guangdong
China

Sui Peng

Jining Medical University - Institute of Precision Medicine

Jining, Shandong 272067
China

Qingchun Tong

University of Texas at Houston, McGovern Medical School, Graduate School of Biological Sciences, Brown Foundation Institute of Molecular Medicine and Program in Neuroscience

Houston, TX
United States

Xingxu Huang

ShanghaiTech University - School of Life Science and Technology

100 Haike Road, Zhangjiang Hi-Tech Park, Pudong
Research Building
Shanghai, 201210
China

Junming Fan (Contact Author)

ShanghaiTech University - School of Life Science and Technology ( email )

100 Haike Road, Zhangjiang Hi-Tech Park, Pudong
Research Building
Shanghai, 201210
China

Haipeng Xiao

Sun Yat-Sen University (SYSU) - Institute of Precision Medicine ( email )

Guangzhou, Guangdong
China

Cheng Huang

Shanghai University of Traditional Chinese Medicine - Drug Discovery Lab ( email )

Shanghai, 201210
China

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