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Peripheral, Intrareceptor Inhibition in Mosquito Olfaction

54 Pages Posted: 21 Nov 2018 Sneak Peek Status: Under Review

See all articles by Pingxi Xu

Pingxi Xu

University of California, Davis - Department of Molecular and Cellular Biology

Young-Moo Choo

University of California, Davis - Department of Molecular and Cellular Biology

Zhou Chen

Auburn University - Department of Entomology and Plant Pathology

Fangfang Zeng

University of California, Davis - Department of Molecular and Cellular Biology

Kaiming Tan

University of California, Davis - Department of Molecular and Cellular Biology

Tsung-Yu Chen

University of California, Davis - Center for Neuroscience

Anthony J. Cornel

University of California, Davis - Department of Entomology and Nematology

Nannan Liu

Auburn University - Department of Entomology and Plant Pathology

Walter Leal

University of California, Davis - Department of Molecular and Cellular Biology

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Abstract

How chemical signals are integrated at the peripheral sensory system of insects is still an enigma. Here we show that when coexpressed with Orco in Xenopus oocytes, an odorant receptor from the southern house mosquito, CquiOR32, generated inward (regular) currents when challenged with cyclohexanone and methyl salicylate, whereas eucalyptol and fenchone elicited inhibitory (upward) currents. Responses of CquiOR32-CquiOrco-expressing oocytes to odorants were reduced in a dose-dependent fashion by coapplication of inhibitors. This intrareceptor inhibition was also manifested in vivo in fruit flies expressing the mosquito receptor CquiOR32, as well in neurons on the antennae of the southern house mosquito. Likewise, an orthologue from the yellow fever mosquito, AaegOR71, showed intrareceptor inhibition in the Xenopus oocyte recording system and corresponding inhibition in antennal neurons. Intrareceptor inhibition was also manifested in mosquito behavior. Blood-seeking females were repelled by methyl salicylate, but repellence was significantly reduced when methyl salicylate was coapplied with eucalyptol.

Keywords: intrareceptor inhibition, intraneuron inhibition, reverse agonist, Culex quinquefasciatus, Aedes aegypti, inhibitor, repellent, methyl salicylate, eucalyptol, outward current

Suggested Citation

Xu, Pingxi and Choo, Young-Moo and Chen, Zhou and Zeng, Fangfang and Tan, Kaiming and Chen, Tsung-Yu and Cornel, Anthony J. and Liu, Nannan and Leal, Walter, Peripheral, Intrareceptor Inhibition in Mosquito Olfaction (November 20, 2018). Available at SSRN: https://ssrn.com/abstract=3287788 or http://dx.doi.org/10.2139/ssrn.3287788
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Pingxi Xu

University of California, Davis - Department of Molecular and Cellular Biology

One Shields Avenue
Davis, CA 95616
United States

Young-Moo Choo

University of California, Davis - Department of Molecular and Cellular Biology

One Shields Avenue
Davis, CA 95616
United States

Zhou Chen

Auburn University - Department of Entomology and Plant Pathology

Auburn, AL 36849
United States

Fangfang Zeng

University of California, Davis - Department of Molecular and Cellular Biology

One Shields Avenue
Davis, CA 95616
United States

Kaiming Tan

University of California, Davis - Department of Molecular and Cellular Biology

One Shields Avenue
Davis, CA 95616
United States

Tsung-Yu Chen

University of California, Davis - Center for Neuroscience

1 Shields Ave
Davis, CA 95616
United States

Anthony J. Cornel

University of California, Davis - Department of Entomology and Nematology

One Shields Avenue
Davis, CA 95616
United States

Nannan Liu

Auburn University - Department of Entomology and Plant Pathology

Auburn, AL 36849
United States

Walter Leal (Contact Author)

University of California, Davis - Department of Molecular and Cellular Biology ( email )

One Shields Avenue
Davis, CA 95616
United States

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