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The IgG Galactosylation Ratio is Higher in Spondyloarthritis Patients and Associated with the MRI and the Response of TNF Blocker

20 Pages Posted: 22 Dec 2018

See all articles by Jing Liu

Jing Liu

Fudan University - State Key Laboratory of Genetic Engineering

Qi Zhu

Shanghai Academy of Chinese Medical Sciences

Jing Han

Fudan University - Department of Biochemistry and Molecular Biology

Hui Zhang

Fudan University - State Key Laboratory of Genetic Engineering

Yanyun Ma

Fudan University, School of Life Sciences, MOE Key Laboratory of Contemporary Anthropology

Hengdong Ji

Nantong University - Taizhou People's Hospital

Dongyi He

Shanghai Academy of Chinese Medical Sciences

Yuan Li

Fudan University - State Key Laboratory of Genetic Engineering

Jianxin Gu

Fudan University - Department of Biochemistry and Molecular Biology

Xiaodong Zhou

University of Texas at Houston

John D. Reveille

University of Texas at Houston

Li Jin

Fudan University - Collaborative Innovation Center for Genetics and Development; Fudan University - State Key Laboratory of Genetic Engineering; Fudan University - Taizhou Institute of Health Sciences; Fudan University - Human Phenome Institute

Hejian Zou

Fudan University

Shifang Ren

Fudan University - Department of Biochemistry and Molecular Biology

Jiucun Wang

Fudan University; Fudan University - Collaborative Innovation Center for Genetics and Development

More...

Abstract

Objectives: MRI is an important tool for evaluating inflammation levels and assessing treatment response in patients with ankylosing spondylitis (AS). However, it is expensive, and requires experienced physicians. The goal of this study was to identify a biomarker correlated with MRI score in assessing treatment response of AS.  

Methods: A total of 558 spondyloarthritis (SpA) patients including 527 AS patients, 10  Psoriasis (PsA) and 21 non-radigographic SpA (nrSpA,) and 725 controls were enrolled for the studies. Plasma IgG galactosylation (IgG-Gal) level was measured by mass spectrometry. Clinical indexes such as Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) and C-reactive protein (CRP) were measured in all AS patients. MRIs and X-rays were obtained from 65 AS patients who were treated with a TNF blocker and longitudinally followed up for 6 months.  

Results: The IgG-Gal ratio was twice as high in the AS patients compared to the controls. It correlated with sacroiliac joint (SIJ) and spine inflammation indices (Pearson coefficient/p value was 0.52/2E10-4 and 0.29/1E-3, respectively). In addition, AS patients with a higher IgG-Gal ratio at baseline tended to show greater improvement in SIJ inflammation scores by MRI, and the IgG-Gal ratio of AS patients was able to predict the response of TNF blocker measured by Assessment of SpondyloArthritis international Society (ASAS40) (AUC = 0.85).  

Conclusion: IgG-Gal ratio was significantly increased in AS patients. In clinical care, it may be used as a potential biomarker for diagnosis and prediction of drug response of AS patients in the future.

Funding Statement: The study was supported by research grants from the National Basic Research Program (2014CB541801), National key research and development program (2016YFA0501303), National Natural Science Foundation of China (31521003, 31630088), Shanghai Municipal Science and Technology Major Project (2017SHZDZX01), International S&T Cooperation Program of China (2013DFA30870), 111 Project (B13016) and US NIH NIAID U01 (1U01AI090909), and China Medical Foundation (CMF). Computational support was provided by the High-End Computing Center located at Fudan University.

Declaration of Interests: All authors declare no conflicts of interest.

Ethics Approval Statement: This study was approved by the Ethical Committees of the School of Life Sciences of Fudan University and all participants provided written informed consent.

Keywords: Spondyloarthritis, MRI imaging, Biomarkers, Laboratory diagnosis, drug response assessment

Suggested Citation

Liu, Jing and Zhu, Qi and Han, Jing and Zhang, Hui and Ma, Yanyun and Ji, Hengdong and He, Dongyi and Li, Yuan and Gu, Jianxin and Zhou, Xiaodong and Reveille, John D. and Jin, Li and Zou, Hejian and Ren, Shifang and Wang, Jiucun, The IgG Galactosylation Ratio is Higher in Spondyloarthritis Patients and Associated with the MRI and the Response of TNF Blocker (March 12, 2018). Available at SSRN: https://ssrn.com/abstract=3296640 or http://dx.doi.org/10.2139/ssrn.3296640

Jing Liu

Fudan University - State Key Laboratory of Genetic Engineering

Shanghai
China

Qi Zhu

Shanghai Academy of Chinese Medical Sciences

Shanghai
China

Jing Han

Fudan University - Department of Biochemistry and Molecular Biology

Shanghai
China

Hui Zhang

Fudan University - State Key Laboratory of Genetic Engineering

Shanghai
China

Yanyun Ma

Fudan University, School of Life Sciences, MOE Key Laboratory of Contemporary Anthropology

China

Hengdong Ji

Nantong University - Taizhou People's Hospital

China

Dongyi He

Shanghai Academy of Chinese Medical Sciences

Shanghai
China

Yuan Li

Fudan University - State Key Laboratory of Genetic Engineering

Shanghai
China

Jianxin Gu

Fudan University - Department of Biochemistry and Molecular Biology

Shanghai
China

Xiaodong Zhou

University of Texas at Houston

Houston, TX 77030
United States

John D. Reveille

University of Texas at Houston

Houston, TX 77030
United States

Li Jin

Fudan University - Collaborative Innovation Center for Genetics and Development

China

Fudan University - State Key Laboratory of Genetic Engineering

Shanghai
China

Fudan University - Taizhou Institute of Health Sciences

Taizhou
China

Fudan University - Human Phenome Institute

Shanghai
China

Hejian Zou

Fudan University

Beijing West District Baiyun Load 10th
Shanghai, 100045
China

Shifang Ren

Fudan University - Department of Biochemistry and Molecular Biology

Shanghai
China

Jiucun Wang (Contact Author)

Fudan University

Beijing West District Baiyun Load 10th
Shanghai, 100045
China

Fudan University - Collaborative Innovation Center for Genetics and Development

China

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