Traditional Medicinal Plants of Higher Altitude of Himachal Pradesh as Functional Food Ingredient Cum Food Preservative and Bioavailability Enhancer of Anifungal Antibiotics
18 Pages Posted: 20 Dec 2018
Date Written: December 11, 2018
Candidiasis is a fungal infection caused by yeasts that belong to the genus Candida. Fluconazole and amphotericin B are most commonly used antibiotics to treat Candidiasis. Some strains of Candida have developed drug resistance (DR) to these antibiotics (and even multidrug resistance, MDR), which are difficult to treat. The present study was undertaken to investigate the potential of four different medicinal plants (Picrorhiza kurroa, Polygonum amplexicaule, Cythula tomentosa, Bergenia ligulata) of high altitude of Himachal Pradesh as bioavailability enhancer of anifungal antibiotics (fluconazole and amphotericin B.) and antioxidant potential to develop functional food ingredient cum food preservatives. Methanolic extracts of P. amplexicaule showed higher total phenolic content (549.33 ± 3.9 mg/gm GAE) and B. ligulata (rhizome extract) showed higher flavonoid content (382.8 ± 2.4 mg/gm RE). Methanolic extracts of B. ligulata (roots and leaves) and P. amplexicaule showed higher antifungal potential as determined by agar well diffusion and broth dilution methods against Candida albicans (MTCC277 and ATCC90028) and Saccharomyces cerevisiae (H1086). Extracts of B. ligulata (leaves and roots) and P. amplexicaule (roots) enhanced the bioavailability of fluconazole and amphotericin B, thus providing better management to Candidiasis. Extracts of all the selected medicinal plants were also screened and compared for in vitro antioxidant potential using DPPH radical scavenging method. It was found that methanolic extracts of P. kurroa (IC50 value 5.62 μg/ml and B. ligulata (roots IC50- 3.107 μg/ml, leaves (3.99μg/ml) showed higher antioxidant potential as compared to other medicinal plants and very similar to ascorbic acid. The data from the current study proposed the importance of these four medicinal plants of higher altitudinal regions of Himachal Pradesh as functional food ingredients and food preservation and bioavailability enhancer to better treat drug resistance in Candida based infections.
Keywords: Candidiasis, Medicinal plants, Bioavailability enhancer, Antifungal, Antioxidant, synergistic, fluconazole
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