Traditional Medicinal Plants of Higher Altitude of Himachal Pradesh as Functional Food Ingredient Cum Food Preservative and Bioavailability Enhancer of Anifungal Antibiotics

18 Pages Posted: 20 Dec 2018

See all articles by Rajan Rolta

Rajan Rolta

Shoolini University - Yeast Biology Laboratory

Akash Sharma

Shoolini University - Yeast Biology Laboratory

Vikas Kumar

Shoolini University - Yeast Biology Laboratory

Pankaj Kumar Chauhan

Shoolini University - Faculty of Applied Sciences and Biotechnology

Anuradha Sourirajan

Shoolini University - Yeast Biology Laboratory

Kamal Dev

Department of Food Science and Nutrition

Date Written: December 11, 2018

Abstract

Candidiasis is a fungal infection caused by yeasts that belong to the genus Candida. Fluconazole and amphotericin B are most commonly used antibiotics to treat Candidiasis. Some strains of Candida have developed drug resistance (DR) to these antibiotics (and even multidrug resistance, MDR), which are difficult to treat. The present study was undertaken to investigate the potential of four different medicinal plants (Picrorhiza kurroa, Polygonum amplexicaule, Cythula tomentosa, Bergenia ligulata) of high altitude of Himachal Pradesh as bioavailability enhancer of anifungal antibiotics (fluconazole and amphotericin B.) and antioxidant potential to develop functional food ingredient cum food preservatives. Methanolic extracts of P. amplexicaule showed higher total phenolic content (549.33 ± 3.9 mg/gm GAE) and B. ligulata (rhizome extract) showed higher flavonoid content (382.8 ± 2.4 mg/gm RE). Methanolic extracts of B. ligulata (roots and leaves) and P. amplexicaule showed higher antifungal potential as determined by agar well diffusion and broth dilution methods against Candida albicans (MTCC277 and ATCC90028) and Saccharomyces cerevisiae (H1086). Extracts of B. ligulata (leaves and roots) and P. amplexicaule (roots) enhanced the bioavailability of fluconazole and amphotericin B, thus providing better management to Candidiasis. Extracts of all the selected medicinal plants were also screened and compared for in vitro antioxidant potential using DPPH radical scavenging method. It was found that methanolic extracts of P. kurroa (IC50 value 5.62 μg/ml and B. ligulata (roots IC50- 3.107 μg/ml, leaves (3.99μg/ml) showed higher antioxidant potential as compared to other medicinal plants and very similar to ascorbic acid. The data from the current study proposed the importance of these four medicinal plants of higher altitudinal regions of Himachal Pradesh as functional food ingredients and food preservation and bioavailability enhancer to better treat drug resistance in Candida based infections.

Keywords: Candidiasis, Medicinal plants, Bioavailability enhancer, Antifungal, Antioxidant, synergistic, fluconazole

Suggested Citation

Rolta, Rajan and Sharma, Akash and Kumar, Vikas and Kumar Chauhan, Pankaj and Sourirajan, Anuradha and Dev, Kamal, Traditional Medicinal Plants of Higher Altitude of Himachal Pradesh as Functional Food Ingredient Cum Food Preservative and Bioavailability Enhancer of Anifungal Antibiotics (December 11, 2018). International Conference on New Horizons in Green Chemistry & Technology (ICGCT) 2018. Available at SSRN: https://ssrn.com/abstract=3299285 or http://dx.doi.org/10.2139/ssrn.3299285

Rajan Rolta

Shoolini University - Yeast Biology Laboratory ( email )

Solan, Himachal Pradesh 173212
India

Akash Sharma

Shoolini University - Yeast Biology Laboratory ( email )

Solan, Himachal Pradesh 173212
India

Vikas Kumar

Shoolini University - Yeast Biology Laboratory ( email )

Solan, Himachal Pradesh 173212
India

Pankaj Kumar Chauhan

Shoolini University - Faculty of Applied Sciences and Biotechnology ( email )

Solan, Himachal Pradesh 173212
India

Anuradha Sourirajan

Shoolini University - Yeast Biology Laboratory ( email )

Solan, Himachal Pradesh 173212
India

Kamal Dev (Contact Author)

Department of Food Science and Nutrition ( email )

420 Delaware St. SE
Minneapolis, MN 55455
United States

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