puc-header

Precise Integration of Direct Cell Reprogramming-Induced Neuronal Cells into Adult Neocortical Circuits

37 Pages Posted: 26 Feb 2019 Sneak Peek Status: Review Complete

See all articles by Fengchao Wang

Fengchao Wang

Beijing Normal University (BNU) - IDG/McGovern Institute for Brain Research

Yueguang Liu

Chinese Academy of Sciences (CAS) - Institute of Neuroscience

Yutong Pang

Beijing Normal University (BNU) - IDG/McGovern Institute for Brain Research

Fei Liu

Chinese Academy of Sciences (CAS) - Institute of Neuroscience

Xiang Li

Beijing Normal University (BNU) - IDG/McGovern Institute for Brain Research

Jiacheng Yuan

Chinese Academy of Sciences (CAS) - Institute of Neuroscience

Yuan Zhang

Beijing Normal University (BNU) - IDG/McGovern Institute for Brain Research

Junlan Geng

Chinese Academy of Sciences (CAS) - Institute of Neuroscience

Junhui Zhang

The 6th Hospital of Ningbo - Department of Joint Surgery

Leping Cheng

Chinese Academy of Sciences (CAS) - Institute of Neuroscience

Xiaohui Zhang

Beijing Normal University (BNU) - IDG/McGovern Institute for Brain Research

More...

Abstract

Recent discovery of in vivo reprogramming of glia cells to neurons by definedneurogenic transcription factors in the adult brain has provided a new approach forneuronal replacement therapy. However, it remains completely unknown whether andhow these direct cell reprogramming-induced neuronal cells (iNs) are functionallyintegrated into the existing neural circuits. We report here that in the adult mouseprimary visual cortex (V1), the iNs, reprogrammed from astrocyte by a singletranscription factor Ascl1, can develop proper distinct visual tuning functions and theyare precisely integrated into the existing retinotopic map, compared to originalcounterparts. Matured iNs receive brain-wide afferent connections that are known toinnervate the V1. They form fully-assembled structures of afferent and efferent synapseswith pre-existing V1 neurons under electron microscopy. Thus, these in vivo findingsstrongly endorse promise for application of direct cell-reprogramming to the neuralcircuit reconstruction in a diseased or injured brain.

Keywords: direct cell programming, astrocyte, induced neurons, functional integration, neocortical circuits, primary visual cortex, visual functions, connection maps, adult brain, neural repair

Suggested Citation

Wang, Fengchao and Liu, Yueguang and Pang, Yutong and Liu, Fei and Li, Xiang and Yuan, Jiacheng and Zhang, Yuan and Geng, Junlan and Zhang, Junhui and Cheng, Leping and Zhang, Xiaohui, Precise Integration of Direct Cell Reprogramming-Induced Neuronal Cells into Adult Neocortical Circuits (December 29, 2018). Available at SSRN: https://ssrn.com/abstract=3307561 or http://dx.doi.org/10.2139/ssrn.3307561
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Fengchao Wang

Beijing Normal University (BNU) - IDG/McGovern Institute for Brain Research

No. 19 Xinjiekouwai Street
Beijing, Haidian 100875
China

Yueguang Liu

Chinese Academy of Sciences (CAS) - Institute of Neuroscience

Wuhan, 430071
China

Yutong Pang

Beijing Normal University (BNU) - IDG/McGovern Institute for Brain Research

No. 19 Xinjiekouwai Street
Beijing, Haidian 100875
China

Fei Liu

Chinese Academy of Sciences (CAS) - Institute of Neuroscience

Wuhan, 430071
China

Xiang Li

Beijing Normal University (BNU) - IDG/McGovern Institute for Brain Research

No. 19 Xinjiekouwai Street
Beijing, Haidian 100875
China

Jiacheng Yuan

Chinese Academy of Sciences (CAS) - Institute of Neuroscience

Wuhan, 430071
China

Yuan Zhang

Beijing Normal University (BNU) - IDG/McGovern Institute for Brain Research

No. 19 Xinjiekouwai Street
Beijing, Haidian 100875
China

Junlan Geng

Chinese Academy of Sciences (CAS) - Institute of Neuroscience

Wuhan, 430071
China

Junhui Zhang

The 6th Hospital of Ningbo - Department of Joint Surgery

Ningbo, Zhejiang 31500
China

Leping Cheng

Chinese Academy of Sciences (CAS) - Institute of Neuroscience ( email )

Wuhan, 430071
China

Xiaohui Zhang (Contact Author)

Beijing Normal University (BNU) - IDG/McGovern Institute for Brain Research ( email )

No. 19 Xinjiekouwai Street
Beijing, Haidian 100875
China

Click here to go to Cell.com

Paper statistics

Abstract Views
442
Downloads
12