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Alterations in Mobility, Survival and Synchrony of Cell Division Close to Biodegradable Iron. Multidimensional Microscopy Analysis Without Use of Stains

25 Pages Posted: 14 Jan 2019 First Look: Preprint

See all articles by NS Fagali

NS Fagali

Republic of Argentina - Institute of Theoretical and Applied Physicochemical Research (INIFTA)

MA Madrid

Republic of Argentina - Department of Mechanical Engineering

BT Pérez Maceda

Republic of Argentina - Cell-Biomaterial Recognition Lab

ME López Fernández

Republic of Argentina - Cell-Biomaterial Recognition Lab

RM Lozano Puerto

Republic of Argentina - Cell-Biomaterial Recognition Lab

M. Fernández Lorenzo

Republic of Argentina - Institute of Theoretical and Applied Physicochemical Research (INIFTA); Universidad Nacional de La Plata - Faculty of Engineering

Abstract

The interactions between macrophages and bioabsorbable metals during their degradation may lead to altered functions of these cells. The evaluation of this impact "in vivo" is tricky and the use of cell cultures is a preliminary valuable model. In the Fe-biodegradation case, the system is complex because Fe-containing materials may lead to corrosion products of different composition and solubility. Thus, a meticulous study about interactions between macrophages and Fe-based materials, without using staining to avoid its non-desired effects, is proposed here. Multidimensional microscopy was applied to obtain images of unstained macrophages (J774A.1) exposed to Feº rings. Images were taken every 15 min between 24-48h of exposure. For the first time for this system software tools for single-cell tracking (TrackMate - FIJI) was applied for the analysis of the spatiotemporal dependence of different parameters. As metal degrades, macrophages capture and accumulate the degradation products as a dark insoluble material. Significant changes in macrophage morphology, motility, synchrony of duplications and in survival of cells, according to the distance to the metal, were noticed. They account for the deep impact of Fe-degradation products on the macrophages close to the Fe-containing implant.

Keywords: Stent, iron, macrophage, biodegradable, tracking cell

Suggested Citation

Fagali, NS and Madrid, MA and Maceda, BT Pérez and Fernández, ME López and Puerto, RM Lozano and Lorenzo, M. Fernández, Alterations in Mobility, Survival and Synchrony of Cell Division Close to Biodegradable Iron. Multidimensional Microscopy Analysis Without Use of Stains (January 10, 2019). Available at SSRN: https://ssrn.com/abstract=3313303

NS Fagali (Contact Author)

Republic of Argentina - Institute of Theoretical and Applied Physicochemical Research (INIFTA) ( email )

Avda. Rivadavia 1917
Buenos Aires, Federal Capital C1033AAJ
Argentina

MA Madrid

Republic of Argentina - Department of Mechanical Engineering

Avda. Rivadavia 1917
Buenos Aires, Federal Capital C1033AAJ
Argentina

BT Pérez Maceda

Republic of Argentina - Cell-Biomaterial Recognition Lab

Calle Ramiro de Maeztu 9
Madrid, 28040
Spain

ME López Fernández

Republic of Argentina - Cell-Biomaterial Recognition Lab ( email )

Calle Ramiro de Maeztu 9
Madrid, 28040
Spain

RM Lozano Puerto

Republic of Argentina - Cell-Biomaterial Recognition Lab

Calle Ramiro de Maeztu 9
Madrid, 28040
Spain

M. Fernández Lorenzo

Republic of Argentina - Institute of Theoretical and Applied Physicochemical Research (INIFTA)

Avda. Rivadavia 1917
Buenos Aires, Federal Capital C1033AAJ
Argentina

Universidad Nacional de La Plata - Faculty of Engineering

7 Nº 776
Buenos Aires, BA 1900
Argentina

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