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TCF7L2 Promotes Osteogenic Differentiation and Boron-Induced Bone Repair Via Lipocalin 2

32 Pages Posted: 14 Jan 2019 First Look: Preprint

See all articles by Chengcheng Yin

Chengcheng Yin

Wuhan University - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST); Wuhan University - Key Laboratory of Oral Biomedicine

Xiaoshi Jia

Wuhan University - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST); Wuhan University - Key Laboratory of Oral Biomedicine

Qin Zhao

Wuhan University - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST); Wuhan University - Key Laboratory of Oral Biomedicine

Zifan Zhao

Wuhan University - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST); Wuhan University - Key Laboratory of Oral Biomedicine

Jinyang Wang

Wuhan University - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST); Wuhan University - Key Laboratory of Oral Biomedicine

Hui Fu

Wuhan University - Department of Anatomy and Embryology

Zubing Li

Wuhan University - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST); Wuhan University - Key Laboratory of Oral Biomedicine

Yufeng Zhang

Wuhan University - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST); Wuhan University - Key Laboratory of Oral Biomedicine

Abstract

Boron-containing mesoporous bioactive glass (B-MBG) scaffolds are capable of promoting osteogenesis during the process of bone defect repair. Despite this, the involving molecular controls are still largely unclear. We identified that transcription factor 7-like 2 (TCF7L2) served as a key effector promoting boron-induced bone regeneration through lipocalin 2. Lipocalin 2 was highly expressed in osteoblasts treated with B-MBG scaffolds extraction than MBG, as well as TCF7L2. Lipocalin 2, as a secreted bone factor, positively affected osteogenic differentiation of MC3T3-E1 and osteogenesis in vivo, which can be induced by TCF7L2. In addition, interference of TCF7L2 decreased the osteogenic differentiation of MC3T3-E1 in vitro, as well as bone mass, and width growth plate in Ubc-Cre;Tcf7l2fl/fl mice. Finally, we identified lipocalin-2 as a pivotal factor that can rescue the poor ability of osteogenic differentiation of MC3T3-E1 in which TCF7L2 gene was knocked down by lentiviral transfection of shRNA. Our findings provide some new insights into the molecular controls of boron-associated bone regeneration and potential therapeutic targets for the treatment of bone defects.

Keywords: Bone regeneration, Boron, lipocalin 2, transcription factor 7-like 2 (transcription factor 4)

Suggested Citation

Yin, Chengcheng and Jia, Xiaoshi and Zhao, Qin and Zhao, Zifan and Wang, Jinyang and Fu, Hui and Li, Zubing and Zhang, Yufeng, TCF7L2 Promotes Osteogenic Differentiation and Boron-Induced Bone Repair Via Lipocalin 2 (January 10, 2019). Available at SSRN: https://ssrn.com/abstract=3313307

Chengcheng Yin (Contact Author)

Wuhan University - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST)

Wuhan, 430071
China

Wuhan University - Key Laboratory of Oral Biomedicine

Wuhan, 430071
China

Xiaoshi Jia

Wuhan University - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST)

Wuhan, 430071
China

Wuhan University - Key Laboratory of Oral Biomedicine

Wuhan, 430071
China

Qin Zhao

Wuhan University - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST)

Wuhan, 430071
China

Wuhan University - Key Laboratory of Oral Biomedicine

Wuhan, 430071
China

Zifan Zhao

Wuhan University - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST)

Wuhan, 430071
China

Wuhan University - Key Laboratory of Oral Biomedicine

Wuhan, 430071
China

Jinyang Wang

Wuhan University - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST)

Wuhan, 430071
China

Wuhan University - Key Laboratory of Oral Biomedicine

Wuhan, 430071
China

Hui Fu

Wuhan University - Department of Anatomy and Embryology

Wuhan, 430071
China

Zubing Li

Wuhan University - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) ( email )

Wuhan, 430071
China

Wuhan University - Key Laboratory of Oral Biomedicine ( email )

Wuhan, 430071
China

Yufeng Zhang

Wuhan University - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) ( email )

Wuhan, 430071
China

Wuhan University - Key Laboratory of Oral Biomedicine ( email )

Wuhan, 430071
China

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