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Detection of New Crimean-Congo Hemorrhagic Fever Virus Genotypes in Ticks Feeding on Deer and Wild Boar, Spain

16 Pages Posted: 16 Jan 2019

See all articles by Alberto Moraga-Fernández

Alberto Moraga-Fernández

Instituto de Investigación en Recursos Cinegéticos IREC

Lara Royo-Hernández

Instituto de Investigación en Recursos Cinegéticos IREC

Miguel A. Habela

University of Extremadura

Francisco Ruiz Fons

Instituto de Investigación en Recursos Cinegéticos IREC

Rafael Calero-Bernal

Universidad Complutense de Madrid (UCM)

Christian Gortazar

Instituto de Investigación en Recursos Cinegéticos IREC

José de la Fuente

Instituto de Investigación en Recursos Cinegéticos IREC

Isabel Fernandez de Mera

Instituto de Investigación en Recursos Cinegéticos IREC - SaBio

More...

Abstract

Background: Crimean-Congo hemorrhagic fever virus (CCHFV) is the causative agent of the severe tick-borne, which is widely distributed worldwide. The CCHFV transmission to humans occurs through tick bite, crushing of engorged ticks, or contact with infected host blood. Previously, CCHFV genotype Africa III was reported in Spain.  

Methods: A total of 613 ticks, including Hyalomma lusitanicum and Dermacentor marginatus, were collected from hunter-harvested wild ungulates in twenty locations throughout southwestern Spain. Tick RNA was analyzed by a nested RT-PCR targeting CCHFV S segment and the amplicons were sequenced.  

Findings: The study reports the presence of CCHFV human genotype Europe V in both tick species collected from red deer (Cervus elaphus), fallow deer (Dama dama), and Eurasian wild boar (Sus scrofa). Viral RNA was detected in 129 of the analyzed ticks. Most of the CCHFV S segment sequences showed 99-100% identity to reported sequences of human isolates from Russia and Iran belonging to genotype Europe V.  

Interpretation: The detection of CCHFV in different tick species collected from various wild host species and localities provided strong evidence of widespread CCHFV presence in the region, suggesting that CCHFV circulation in Spain requires more attention. Additionally, the identification of the CCHFV genotype Europe V in ticks suggested that its introduction in Spain was probably from Eastern Europe.  

Funding: This study was partially supported by MINECO Plan Nacional grant CGL2017-89866-R and EU-FEDER.  

Declaration of Interest: The authors declare no commercial or financial conflict of interest.

Ethical Approval: Tick sampling were collected according to hunting disease surveillance schemes and in the framework of Spanish and EU laws for notifiable disease surveillance. Therefore, no Animal Ethics Committee approval was required for the collection of ticks from hunter harvested wild ungulates.

Keywords: Arbovirus, Crimean-Congo hemorrhagic fever, ticks, ungulates, vector-borne infections, wildlife, zoonoses

Suggested Citation

Moraga-Fernández, Alberto and Royo-Hernández, Lara and Habela, Miguel A. and Ruiz Fons, Francisco and Calero-Bernal, Rafael and Gortazar, Christian and de la Fuente, José and Fernandez de Mera, Isabel, Detection of New Crimean-Congo Hemorrhagic Fever Virus Genotypes in Ticks Feeding on Deer and Wild Boar, Spain (September 1, 2019). Available at SSRN: https://ssrn.com/abstract=3314445

Alberto Moraga-Fernández

Instituto de Investigación en Recursos Cinegéticos IREC

Ciudad Real
Spain

Lara Royo-Hernández

Instituto de Investigación en Recursos Cinegéticos IREC

Ciudad Real
Spain

Miguel A. Habela

University of Extremadura

Avenida de la Universidad s/n
Caceres, 10071
Spain

Francisco Ruiz Fons

Instituto de Investigación en Recursos Cinegéticos IREC

Ciudad Real
Spain

Rafael Calero-Bernal

Universidad Complutense de Madrid (UCM)

Carretera de Humera s/n
Madrid, Madrid 28223
Spain

Christian Gortazar

Instituto de Investigación en Recursos Cinegéticos IREC

Ciudad Real
Spain

José De la Fuente

Instituto de Investigación en Recursos Cinegéticos IREC

Ciudad Real
Spain

Isabel Fernandez de Mera (Contact Author)

Instituto de Investigación en Recursos Cinegéticos IREC - SaBio ( email )

Ronda de Toledo 12
Ciudad Real, 13005
Spain

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