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Induction of Pluripotency by Alternative Factors

46 Pages Posted: 26 Feb 2019 Sneak Peek Status: Review Complete

See all articles by Bo Wang

Bo Wang

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Linlin Wu

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Dongwei Li

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Yuting Liu

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Jing Guo

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Chen Li

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Guoqing Zhao

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Yuxiang Yao

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Xiaoshan Wang

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Meijun Fu

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Yaofeng Wang

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

He Liu

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Shangtao Cao

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Chuman Wu

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Shengyong Yu

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Chunhua Zhou

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Junqi Kuang

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Jin Ming

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Xuejie Yang

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Jiekai Chen

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Jing Liu

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Duanqing Pei

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

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Abstract

Reprogramming somatic cells to pluripotency represents a paradigm for cell fate determination. A binary logic of closing and opening chromatin provides a simple way to understand iPSC reprogramming driven by both Yamanaka factors or chemicals. Here we apply this logic to design a combination of Jdp2, Jhdm1b, Mkk6, Glis1, Nanog, Essrb and Sall4 (7F) that reprogram MEFs to chimera competent iPSCs efficiently. RNA- and ATAC-seq reveal distinct mechanisms for 7F induction of pluripotency. Dropout experiments further reveal a highly cooperative process among 7F to dynamically close and open chromatin loci that encode a network of transcription factors to mediate reprogramming. These results reveal a previously unknown path between somatic and pluripotent states and open new doors for cell fate control.

Suggested Citation

Wang, Bo and Wu, Linlin and Li, Dongwei and Liu, Yuting and Guo, Jing and Li, Chen and Zhao, Guoqing and Yao, Yuxiang and Wang, Xiaoshan and Fu, Meijun and Wang, Yaofeng and Liu, He and Cao, Shangtao and Wu, Chuman and Yu, Shengyong and Zhou, Chunhua and Kuang, Junqi and Ming, Jin and Yang, Xuejie and Chen, Jiekai and Liu, Jing and Pei, Duanqing, Induction of Pluripotency by Alternative Factors (January 12, 2019). Available at SSRN: https://ssrn.com/abstract=3314565 or http://dx.doi.org/10.2139/ssrn.3314565
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Bo Wang

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Guangzhou, 510530
China

Linlin Wu

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Guangzhou, 510530
China

Dongwei Li

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Guangzhou, 510530
China

Yuting Liu

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Guangzhou, 510530
China

Jing Guo

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Guangzhou, 510530
China

Chen Li

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Guangzhou, 510530
China

Guoqing Zhao

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Guangzhou, 510530
China

Yuxiang Yao

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Guangzhou, 510530
China

Xiaoshan Wang

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Guangzhou, 510530
China

Meijun Fu

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Guangzhou, 510530
China

Yaofeng Wang

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Guangzhou, 510530
China

He Liu

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Guangzhou, 510530
China

Shangtao Cao

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Guangzhou, 510530
China

Chuman Wu

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Guangzhou, 510530
China

Shengyong Yu

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Guangzhou, 510530
China

Chunhua Zhou

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Guangzhou, 510530
China

Junqi Kuang

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Guangzhou, 510530
China

Jin Ming

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Guangzhou, 510530
China

Xuejie Yang

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Guangzhou, 510530
China

Jiekai Chen

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Guangzhou, 510530
China

Jing Liu

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Guangzhou, 510530
China

Duanqing Pei (Contact Author)

Chinese Academy of Sciences (CAS) - Key Laboratory of Regenerative Biology

Guangzhou, 510530
China

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