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A Positive Feedback Mechanism Drives Membrane Ingression During Cytokinesis in an Actomyosin-Ring Independent Manner

27 Pages Posted: 26 Feb 2019 Sneak Peek Status: Review Complete

See all articles by Franz Meitinger

Franz Meitinger

Heidelberg University - Centre for Organismal Studies (COS); German Cancer Research Center (DKFZ) - DKFZ-ZMBH Alliance; Ludwig Institute for Cancer Research

Gislene Pereira

Heidelberg University - Centre for Organismal Studies (COS); German Cancer Research Center (DKFZ) - DKFZ-ZMBH Alliance

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Abstract

The molecular mechanism underlying actomyosin-ring (AMR) driven furrow ingression during cytokinesis is still unclear. We functionally separated sub-molecular domains of the AMR and associated proteins, which are important to maintain the integrity of the AMR, from domains that are important to drive plasma membrane ingression. Specific mutations that interrupt the interactions between Myosin II, the essential myosin light chain, IQGAP1 and the F-BAR protein Hof1 destabilize the cytokinetic AMR and prevent centripetal furrow ingression, but still allow asymmetric plasma membrane ingression with a velocity that is comparable to cells with a functional AMR. Our data indicate that the AMR directs ingression factors to the cytokinetic furrow to initiate a positive feedback mechanism that drives membrane ingression, which is independent of AMR contractility.

Suggested Citation

Meitinger, Franz and Pereira, Gislene, A Positive Feedback Mechanism Drives Membrane Ingression During Cytokinesis in an Actomyosin-Ring Independent Manner (January 16, 2019). Available at SSRN: https://ssrn.com/abstract=3316805 or http://dx.doi.org/10.2139/ssrn.3316805
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Franz Meitinger

Heidelberg University - Centre for Organismal Studies (COS)

Im Neuenheimer Feld 230
Heidelberg, 69120
Germany

German Cancer Research Center (DKFZ) - DKFZ-ZMBH Alliance

Im Neuenheimer Feld 230
Heidelberg, 69120
Germany

Ludwig Institute for Cancer Research

666 Third Avenue, 28th floor
New York
NY 10017
United States

Gislene Pereira (Contact Author)

Heidelberg University - Centre for Organismal Studies (COS) ( email )

Im Neuenheimer Feld 230
Heidelberg, 69120
Germany

German Cancer Research Center (DKFZ) - DKFZ-ZMBH Alliance ( email )

Im Neuenheimer Feld 230
Heidelberg, 69120
Germany

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