A Positive Feedback Mechanism Drives Membrane Ingression During Cytokinesis in an Actomyosin-Ring Independent Manner
27 Pages Posted: 26 Feb 2019 Sneak Peek Status: Review CompleteMore...
The molecular mechanism underlying actomyosin-ring (AMR) driven furrow ingression during cytokinesis is still unclear. We functionally separated sub-molecular domains of the AMR and associated proteins, which are important to maintain the integrity of the AMR, from domains that are important to drive plasma membrane ingression. Specific mutations that interrupt the interactions between Myosin II, the essential myosin light chain, IQGAP1 and the F-BAR protein Hof1 destabilize the cytokinetic AMR and prevent centripetal furrow ingression, but still allow asymmetric plasma membrane ingression with a velocity that is comparable to cells with a functional AMR. Our data indicate that the AMR directs ingression factors to the cytokinetic furrow to initiate a positive feedback mechanism that drives membrane ingression, which is independent of AMR contractility.
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