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Mapping Optogenetically Driven Single-Vessel fMRI with Concurrent Neuronal Calcium Recordings in the Rat Hippocampus

47 Pages Posted: 24 Jan 2019 Publication Status: Review Complete

See all articles by Xuming Chen

Xuming Chen

Max Planck Institute for Biological Cybernetics

Filip Sobcazk

Max Planck Institute for Biological Cybernetics

Yi Chen

Max Planck Institute for Biological Cybernetics

Chunqi Qian

Michigan State University - Department of Radiology

Zuneng Lu

Wuhan University - Department of Neurology

Cenk Ayata

Massachusetts General Hospital - Department of Neurology

Nikos K. Logothetis

Max Planck Institute for Biological Cybernetics

Xin Yu

Max Planck Institute for Biological Cybernetics

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Abstract

The hippocampus plays critical roles in learning and memory, as well as pathological states such as epilepsy and Alzheimer’s disease. To better understand its neural network function, the multi-modality methodology has been implemented in both in vitro and in vivo conditions. However, the large-scale hippocampal vascular hemodynamic responses linked to specific neural activity remains to be elucidated at the single-vessel level through the hippocampus. We merged the fiber optic calcium recording with optogenetically driven single-vessel fMRI to obtain concurrent neuronal calcium signal with blood-oxygen-level-dependent and cerebral-blood-volume (CBV) signal from individual venules and arterioles, respectively, which are aligned in an interleaved pattern to circulate blood through the hippocampus. This multi-modal fMRI platform detects specific spatiotemporal hemodynamic patterns from hippocampal vasculature correlated with physiologically evoked and spreading depression-like calcium events. The calcium event-related single-vessel fMRI hemodynamic modeling reveals significantly reduced neurovascular coupling efficiency upon spreading depression-like events, providing a direct measure of the hippocampal vascular function at varied states in animal models.

Suggested Citation

Chen, Xuming and Sobcazk, Filip and Chen, Yi and Qian, Chunqi and Lu, Zuneng and Ayata, Cenk and Logothetis, Nikos K. and Yu, Xin, Mapping Optogenetically Driven Single-Vessel fMRI with Concurrent Neuronal Calcium Recordings in the Rat Hippocampus (January 19, 2019). Available at SSRN: https://ssrn.com/abstract=3318946 or http://dx.doi.org/10.2139/ssrn.3318946
This version of the paper has not been formally peer reviewed.

Xuming Chen (Contact Author)

Max Planck Institute for Biological Cybernetics

Tübingen
Germany

Filip Sobcazk

Max Planck Institute for Biological Cybernetics

Tübingen
Germany

Yi Chen

Max Planck Institute for Biological Cybernetics

Tübingen
Germany

Chunqi Qian

Michigan State University - Department of Radiology ( email )

232 Radiology Building
East Lansing, MI 48824
United States

Zuneng Lu

Wuhan University - Department of Neurology

Wuhan, Hubei
China

Cenk Ayata

Massachusetts General Hospital - Department of Neurology

55 Fruit Street Boston
Wang Ambulatory Care Center, 8th Floor, Suite 835
Boston, MA 02114
United States

Nikos K. Logothetis

Max Planck Institute for Biological Cybernetics

Tübingen
Germany

Xin Yu

Max Planck Institute for Biological Cybernetics ( email )

Tübingen
Germany

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