Mapping Optogenetically Driven Single-Vessel fMRI with Concurrent Neuronal Calcium Recordings in the Rat Hippocampus
47 Pages Posted: 24 Jan 2019 Publication Status: Review CompleteMore...
The hippocampus plays critical roles in learning and memory, as well as pathological states such as epilepsy and Alzheimer’s disease. To better understand its neural network function, the multi-modality methodology has been implemented in both in vitro and in vivo conditions. However, the large-scale hippocampal vascular hemodynamic responses linked to specific neural activity remains to be elucidated at the single-vessel level through the hippocampus. We merged the fiber optic calcium recording with optogenetically driven single-vessel fMRI to obtain concurrent neuronal calcium signal with blood-oxygen-level-dependent and cerebral-blood-volume (CBV) signal from individual venules and arterioles, respectively, which are aligned in an interleaved pattern to circulate blood through the hippocampus. This multi-modal fMRI platform detects specific spatiotemporal hemodynamic patterns from hippocampal vasculature correlated with physiologically evoked and spreading depression-like calcium events. The calcium event-related single-vessel fMRI hemodynamic modeling reveals significantly reduced neurovascular coupling efficiency upon spreading depression-like events, providing a direct measure of the hippocampal vascular function at varied states in animal models.
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