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miR-192/215-5p Act as Tumor Suppressors and Link Crohn's Disease and Colorectal Cancer by Targeting Metabolic Pathways: A Comprehensive Database Mining and Experimental Study

33 Pages Posted: 24 Jan 2019

See all articles by Hu Zhao

Hu Zhao

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology

Xiaofeng Lai

Xiamen University

Jin Chen

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology

Xuhui Kong

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology

Hehuan Zhu

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology

Yongping Zhang

University of Texas at Houston, Health Science Center at Houston (UTHealth), MD Anderson Cancer Center, Department of Neuro-Oncology

Qinghua Wang

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology

Shushang Chen

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology

Zhen Den

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology

Huiyue Dong

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology

Zhan Chen

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology

Qiang Cui

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology

Junqiong Zheng

Fujian Medical University

Jun Lu

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology; Fujian Hongyi Health Institute

Shuiliang Wang

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology; Fujian Hongyi Health Institute

Jianming Tan

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology; Fujian Hongyi Health Institute

More...

Abstract

Background: MicroRNAs (miRNAs) have emerged as key regulators involved in a variety of biological processes such as inflammatory bowel diseases and tumor progression. Previous studies have demonstrated that miR-192/215 participated in progression of Crohn's disease (CD) and colorectal cancer (CRC), respectively; however, their detailed functional relationships and molecular regulations between two diseases remain unclear.

Methods: Here we used bioinformatics methods to systematically expound miR-192/215-5p macrocontrol regulatory networks shared by two diseases. For data mining and validation, several miRNA prediction tools including Tarbase, miRTARBASE, miRecored, miRpathDB, et al; Human miRNA tissue atlas, FunRich, miRcancer, MalaCards, GEPIA, cBioPortal, luciferase assay, et al. are extensively applied.

Findings: miR-192/215-5p with identical seed sequence were specially distributed in colon tissues and enriched biological pathways were closely associated with human cancers. Emerging role of miR-192/215-5p in CD and CRC was also analyzed. In addition, we analyzed common pathways targeted by miR-192/215-5p in both diseases.

Interpretation: Based on results derived from multiple approaches, we identified the biological functions of miR-192/215-5p as a tumor suppressor and link CD and CRC by targeting triglyceride synthesis and extracellular matrix remodeling pathways.

Funding Statement: The research was supported by the Natural Science Foundation of Fujian Province [grant numbers 2012YZ0001-1 and 2016J01582], National Nature Science Foundation of China [grant numbers No.81402107 and No.81601618], Military Medical Science project [grant number No.15QNP025].

Declaration of Interests: No potential conflicts of interest were disclosed.

Ethics Approval Statement: The study protocol was approved by the Ethics Committee of Fuzhou General Hospital, Xiamen University. Data collections and processions were performed according to policies of GEO and TCGA project.

Keywords: microRNA (miR)-192/215-5p; Crohn's disease; Colorectal cancer; Bioinformatics

Suggested Citation

Zhao, Hu and Lai, Xiaofeng and Chen, Jin and Kong, Xuhui and Zhu, Hehuan and Zhang, Yongping and Wang, Qinghua and Chen, Shushang and Den, Zhen and Dong, Huiyue and Chen, Zhan and Cui, Qiang and Zheng, Junqiong and Lu, Jun and Wang, Shuiliang and Tan, Jianming, miR-192/215-5p Act as Tumor Suppressors and Link Crohn's Disease and Colorectal Cancer by Targeting Metabolic Pathways: A Comprehensive Database Mining and Experimental Study (January 21, 2019). Available at SSRN: https://ssrn.com/abstract=3320277

Hu Zhao (Contact Author)

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology ( email )

China

Xiaofeng Lai

Xiamen University

Xiamen, Fujian 361005
China

Jin Chen

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology

China

Xuhui Kong

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology

China

Hehuan Zhu

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology

China

Yongping Zhang

University of Texas at Houston, Health Science Center at Houston (UTHealth), MD Anderson Cancer Center, Department of Neuro-Oncology

TX
United States

Qinghua Wang

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology

China

Shushang Chen

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology

China

Zhen Den

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology

China

Huiyue Dong

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology

China

Zhan Chen

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology

China

Qiang Cui

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology

China

Junqiong Zheng

Fujian Medical University

Fuzhou
China

Jun Lu

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology ( email )

China

Fujian Hongyi Health Institute ( email )

China

Shuiliang Wang

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology ( email )

China

Fujian Hongyi Health Institute

China

Jianming Tan

Xiamen University - Fujian Provincial Key Laboratory of Transplant Biology ( email )

China

Fujian Hongyi Health Institute

China

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