Synergistic Fungicidal Activity of Acapulco (Cassia alata) Leaf Extract and Calamansi (Citrofortunella microcarpa) Fruit Extract Against Magnaporthe oryzae on Rice (Oryzae sativa)
Posted: 6 Feb 2019 Last revised: 19 Feb 2019
Date Written: January 22, 2019
Rice blast, caused by Magnaporthe oryzae, has been considered as the main cause of rice production deficits in the world. Certain fungicides are available nowadays to combat the disease but pose threats to the environment and human health. Thus, this study aims to determine if Acalpulco (Cassia alata) and Calamansi (Citofortunella microcarpa), can be used in efficiently eradicating Magnaporthe oryzae fungi. Calamansi fruits were grounded and filtered while Acapulco leaves were boiled in distilled water for the extraction process. Magnaporthe oryzae isolates were cultured in petri dish with Potato Dextrose Agar for 5 days. Agar diffusion assay was then carried out by putting 2 mL of the treatments mainly: Acapulco Leaf Extract, Calamansi Fruit Extract, combination of Acapulco and Calamansi extracts, Fungitox, and Distilled Water on sterilized filter paper discs of 6 mm in diameter. Filter paper disc was placed across the fungal isolate. Fungal Zone of Inhibition was then measured in millimeter after 7 days using a caliper. Results showed that the fungicidal property of the combination of Acapulco and Calamansi extracts were comparable fungicidal performance of the positive control with an average ZOI of 24.67 mm. There was also a significant difference between the experimental setups being prepared as compared to the controls. After the data was analysed and interpreted, it was concluded that there was a synergy between the Acapulco (Cassia Alata) leaf extract and Calamansi (Citrofortunella microcarpa) fruit extract. It is recommended to test the samples in this study in an in vivo experiment.
Keywords: Magnaporthe oryzae, Acapulco, Calamansi, Fungicide
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