lancet-header
Preprints with The Lancet is part of SSRN´s First Look, a place where journals and other research experts identify content of interest prior to publication. These preprint papers are not peer-reviewed and are posted here as part of a 12-month trial. Authors have either opted in at submission to The Lancet family of journals to post their preprints on Preprints with The Lancet, or submitted directly via SSRN. The usual SSRN checks and a Lancet-specific check for appropriateness and transparency have been applied. These papers should not be used for clinical decision making or reporting of research to a lay audience without indicating that this is preliminary research that has not been peer-reviewed. For more information see the Comment published in The Lancet, or visit The Lancet´s FAQ page, and for any feedback please contact preprints@lancet.com

Lnc-TCL6 is a Potential Biomarker for Early Diagnosis and Grade in Liver Cirrhosis Patients

36 Pages Posted: 14 Mar 2019

See all articles by Leijia Li

Leijia Li

Sun Yat-Sen University (SYSU) - Department of Gastroenterology

Xiaoying Wu

Sun Yat-Sen University (SYSU)

Siwei Tan

Sun Yat-Sen University (SYSU) - Department of Gastroenterology

Zijun Xie

Sun Yat-Sen University (SYSU) - Department of Gastroenterology

Xuemei Pan

Sun Yat-Sen University (SYSU) - Department of Gastroenterology

Shunwen Pan

Sun Yat-Sen University (SYSU) - Department of Gastroenterology

Wurina Bai

Sun Yat-Sen University (SYSU) - Department of Gastroenterology

Haijiao Li

Sun Yat-Sen University (SYSU) - Department of Gastroenterology

Huiling Liu

Sun Yat-Sen University (SYSU) - Department of Gastroenterology

Jie Jiang

Sun Yat-Sen University (SYSU) - Department of Gastroenterology

Bin Wu

Sun Yat-Sen University (SYSU) - Department of Gastroenterology; Guangdong Provincial Key Laboratory of Liver Disease Research

More...

Abstract

Background: Long non-coding RNAs (LncRNAs) have been proved to participate in the pathogenesis or as biomarkers in many diseases. The study aimed to identify some sensitive and noninvasive biomarkers for early diagnosis and grade in liver cirrhosis (LC) patients, especially in hepatitis B liver cirrhosis patients.    

Methods: LncRNAs were screened in whole blood samples by CapitalBiotech microarray in both healthy controls and LC patients to discover candidate biomarkers, and candidate biomarkers were subjected to training using qRT-PCR for the quantification of their expression levels in independent whole blood sample sets, including healthy controls, hepatitis B virus carriers (HBV carrier), chronic hepatitis B (CHB), and LC. Ultimately, an excellent biomarker was validated by Mann-Whitney test and ROC analysis for LC.    

Findings: Lnc-TCL6 was identified as a high diagnostic accurate biomarker for LC in Child-Pugh class C patients compared to healthy controls, CHB and HBV carrier (AUC=0.719, 0.815, 0.797, respectively). Meanwhile, it showed a favorable specificities and sensitivities in Child-Pugh A, B, C grade in LC patients (AUC=0.711, 0.724, 0.837, respectively). Compared to healthy controls, the expression of Lnc-TCL6 was obviously upregulated in Child-Pugh class A (4.07±3.28 vs 2.89±3.16, P=0.014). Conversely, the Lnc-TCL6 was significantly downregulated in Child-Pugh class C patients (1.19±2.09 vs 2.89±3.16, P<0.01).

Interpretation: Lnc-TCL6 is a potential biomarker for early diagnosis and grade in LC patients, and a reliable predictor for poor prognosis in Child-Pugh class C LC patients.  

Funding Statement: This study was supported by National Natural Science Foundation of China (U1501224), the Science and Technology Developmental Special Foundation of Guangdong Province (2017B020226003), the Guangzhou City Science and Technology Program (201604020118), and the Projects of Guangzhou City Health Care Collaborative Innovation (201604020002).

Declaration of Interests: The authors declare no competing interests.

Ethics Approval Statement: The protocol for this clinical trial was approved by the institutional review boards or ethics committees from our hospital and written consent for the information to be stored in the hospital database and used for research was obtained from the study participants.

Keywords: Long non-coding RNA; TCL6; Liver cirrhosis; Child-Pugh classification; Diagnosis

Suggested Citation

Li, Leijia and Wu, Xiaoying and Tan, Siwei and Xie, Zijun and Pan, Xuemei and Pan, Shunwen and Bai, Wurina and Li, Haijiao and Liu, Huiling and Jiang, Jie and Wu, Bin, Lnc-TCL6 is a Potential Biomarker for Early Diagnosis and Grade in Liver Cirrhosis Patients (January 22, 2019). Available at SSRN: https://ssrn.com/abstract=3321481

Leijia Li

Sun Yat-Sen University (SYSU) - Department of Gastroenterology

No. 600 Tianhe Road
Guangzhou, 510630
China

Xiaoying Wu

Sun Yat-Sen University (SYSU)

135, Xingang Xi Road
Guangzhou, Guangdong 510275
China

Siwei Tan

Sun Yat-Sen University (SYSU) - Department of Gastroenterology

No. 600 Tianhe Road
Guangzhou, 510630
China

Zijun Xie

Sun Yat-Sen University (SYSU) - Department of Gastroenterology

No. 600 Tianhe Road
Guangzhou, 510630
China

Xuemei Pan

Sun Yat-Sen University (SYSU) - Department of Gastroenterology

No. 600 Tianhe Road
Guangzhou, 510630
China

Shunwen Pan

Sun Yat-Sen University (SYSU) - Department of Gastroenterology

No. 600 Tianhe Road
Guangzhou, 510630
China

Wurina Bai

Sun Yat-Sen University (SYSU) - Department of Gastroenterology

No. 600 Tianhe Road
Guangzhou, 510630
China

Haijiao Li

Sun Yat-Sen University (SYSU) - Department of Gastroenterology

No. 600 Tianhe Road
Guangzhou, 510630
China

Huiling Liu

Sun Yat-Sen University (SYSU) - Department of Gastroenterology

No. 600 Tianhe Road
Guangzhou, 510630
China

Jie Jiang

Sun Yat-Sen University (SYSU) - Department of Gastroenterology

No. 600 Tianhe Road
Guangzhou, 510630
China

Bin Wu (Contact Author)

Sun Yat-Sen University (SYSU) - Department of Gastroenterology ( email )

No. 600 Tianhe Road
Guangzhou, 510630
China

Guangdong Provincial Key Laboratory of Liver Disease Research ( email )

Guangzhou
China

Click here to go to TheLancet.com

Go to TheLancet.com

Paper statistics

Abstract Views
35
Downloads
6