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Tendril Climber Inspired Structure-Induced Cell Growth by Direct Writing Heterogeneous Scaffold

19 Pages Posted: 28 Jan 2019 First Look: Preprint

See all articles by Chaoqi Xie

Chaoqi Xie

Zhejiang University - State Key Laboratory of Fluid Power and Mechatronic Systems; Zhejiang University - Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province

Qing Gao

Zhejiang University - State Key Laboratory of Fluid Power and Mechatronic Systems; Zhejiang University - Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province

Peng Wang

Zhejiang University - State Key Laboratory of Fluid Power and Mechatronic Systems; Zhejiang University - Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province

Lei Shao

Zhejiang University - State Key Laboratory of Fluid Power and Mechatronic Systems; Zhejiang University - Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province

Huipu Yuan

Zhejiang University - Institute of Translational Medicine

Jianzhong Fu

Zhejiang University - State Key Laboratory of Fluid Power and Mechatronic Systems; Zhejiang University - Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province

Wei Chen

Zhejiang University - Institute of Translational Medicine

Yong He

Zhejiang University - State Key Laboratory of Fluid Power and Mechatronic Systems; Zhejiang University - Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province

Abstract

As a fundamental issue, cell-scaffold interaction has drawn increased attention in tissue engineering. The ability of tendril-climbers to perceive position and climb up toward the trellis in an ingenious manner induces interest. Thus, the question arises whether the cell can also grow as ingenious as the plant. Prompted by the climbing mechanism of tendril climbers, we proposed a novel method for inducing cell growth by using specially designed scaffolds with heterogeneous structures. A high-resolution 3D printing method via melt direct writing for fabricating these scaffolds was developed. By melting biodegradable polymers in the nozzle and high-voltage attraction, scaffolds with fiber diameters measuring 3μm can be printed layer by layer. Heterogeneous structures, such as various fiber diameters and pore sizes, can be freely printed in one scaffold at the different locations by adjusting correlated parameters. Owing to these properties of the scaffold, interesting phenomena of cell growth were observed. Human umbilical vein endothelial cells (HUVECs) exhibited different growth rates on the scaffold with different pore sizes. And bone marrow stem cell (BMSCs) showed several morphological characteristics on the scaffold consisting of fibers with specific diameters. Therefore, we can regulate and control cell growth to different status in one scaffold by merely designing structures. This study generally provides a structure-induced cell growth strategy for better simulating in-vivo like environment.

Keywords: 3D printing, Tissue engineering, Scaffolds, Melt direct writing, Heterogeneous structure, Cell growth

Suggested Citation

Xie, Chaoqi and Gao, Qing and Wang, Peng and Shao, Lei and Yuan, Huipu and Fu, Jianzhong and Chen, Wei and He, Yong, Tendril Climber Inspired Structure-Induced Cell Growth by Direct Writing Heterogeneous Scaffold (January 24, 2019). Available at SSRN: https://ssrn.com/abstract=3321957

Chaoqi Xie (Contact Author)

Zhejiang University - State Key Laboratory of Fluid Power and Mechatronic Systems

38 Zheda Road
Hangzhou, Zhejiang 310058
China

Zhejiang University - Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province

38 Zheda Road
Hangzhou, Zhejiang 310058
China

Qing Gao

Zhejiang University - State Key Laboratory of Fluid Power and Mechatronic Systems

38 Zheda Road
Hangzhou, Zhejiang 310058
China

Zhejiang University - Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province

38 Zheda Road
Hangzhou, Zhejiang 310058
China

Peng Wang

Zhejiang University - State Key Laboratory of Fluid Power and Mechatronic Systems

38 Zheda Road
Hangzhou, Zhejiang 310058
China

Zhejiang University - Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province

38 Zheda Road
Hangzhou, Zhejiang 310058
China

Lei Shao

Zhejiang University - State Key Laboratory of Fluid Power and Mechatronic Systems

38 Zheda Road
Hangzhou, Zhejiang 310058
China

Zhejiang University - Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province

38 Zheda Road
Hangzhou, Zhejiang 310058
China

Huipu Yuan

Zhejiang University - Institute of Translational Medicine

China

Jianzhong Fu

Zhejiang University - State Key Laboratory of Fluid Power and Mechatronic Systems

38 Zheda Road
Hangzhou, Zhejiang 310058
China

Zhejiang University - Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province

38 Zheda Road
Hangzhou, Zhejiang 310058
China

Wei Chen

Zhejiang University - Institute of Translational Medicine

China

Yong He

Zhejiang University - State Key Laboratory of Fluid Power and Mechatronic Systems ( email )

38 Zheda Road
Hangzhou, Zhejiang 310058
China

Zhejiang University - Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province ( email )

38 Zheda Road
Hangzhou, Zhejiang 310058
China

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