Highly Efficient Carboxylated Graphene Oxide-Based Surface Plasmon Resonance Aptasensors Detect hCG Protein to Diagnose Down's Syndrome in Clinical Serum Samples

27 Pages Posted: 28 Jan 2019

See all articles by Nan-Fu Chiu

Nan-Fu Chiu

National Taiwan Normal University - Laboratory of Nano-photonics and Biosensors

Wei-Ren Wu

National Taiwan Normal University - Laboratory of Nano-photonics and Biosensors

Hung-Shun Liao

Mackay Memorial Hospital - Department of Obstetrics and Gynecology; Mackay Medical College - Department of Medicine

Chen-Yu Chen

Mackay Memorial Hospital - Department of Obstetrics and Gynecology; Mackay Medical College - Department of Medicine

Date Written: January 25, 2019

Abstract

This study developed a surface plasmon resonance (SPR) aptasensor suitable for the detection of clinical serum biomarkers. Studies in precision medicine clearly emphasize the need for a carboxyl-functionalized graphene oxide (GO) biosensing material that can rapidly and accurately detect a low concentration of biomolecules to identify various diseases. The developed carboxyl-GO-based SPR aptasensor showed excellent sensitivity and limit of detection without the use of antibodies and without any specific preference. An hCG protein detection limit of 1 pg/mL in buffer samples and 1.9 pM in clinical serum samples were achieved. The results showed that the carboxyl-GO-based chip was well below the normal physiological level of serum protein (0.5 ng/mL). A high correlation coefficient was obtained in the range of 1.9 pM to 135 pM. The results showed a serum hCG concentration of 30.18 to 135 pM with a 5k-fold dilution factor, and that an SPR angle shift of more than 30 millidegrees (m°) was associated with a significant risk of fetal Down's syndrome (DS) compared to normal pregnant women. The results clearly showed that the detection of hCG protein in serum samples from pregnant women at 12-19 weeks can diagnosis Down's syndrome with high selectivity and sensitivity. Our findings suggest the potential application of carboxyl-GO film in proof-of-concept studies for serum assays as a new type of SPR material, and that peptide and carboxyl-GO films may be conducive to the development of future point of care testing and rapid diagnostic devices for a clinical cancer diagnosis and prognosis.

Keywords: surface plasmon resonance (SPR), Aptasensor, Carboxyl-functionalized graphene oxide (GO-COOH), Peptides, human chorionic gonadotropin (hCG), Down's syndrome (DS)

Suggested Citation

Chiu, Nan-Fu and Wu, Wei-Ren and Liao, Hung-Shun and Chen, Chen-Yu, Highly Efficient Carboxylated Graphene Oxide-Based Surface Plasmon Resonance Aptasensors Detect hCG Protein to Diagnose Down's Syndrome in Clinical Serum Samples (January 25, 2019). Available at SSRN: https://ssrn.com/abstract=3321960 or http://dx.doi.org/10.2139/ssrn.3321960

Nan-Fu Chiu (Contact Author)

National Taiwan Normal University - Laboratory of Nano-photonics and Biosensors ( email )

No. 162, Section 1
Heping East Road
Taipei City, Da’an District 106
Taiwan

Wei-Ren Wu

National Taiwan Normal University - Laboratory of Nano-photonics and Biosensors

No. 162, Section 1
Heping East Road
Taipei City, Da’an District 106
Taiwan

Hung-Shun Liao

Mackay Memorial Hospital - Department of Obstetrics and Gynecology

#92, Sec. 2, Chung Shan N. Rd.
Taipei, 10449
Taiwan

Mackay Medical College - Department of Medicine

Taipei, 252
Taiwan

Chen-Yu Chen

Mackay Memorial Hospital - Department of Obstetrics and Gynecology ( email )

#92, Sec. 2, Chung Shan N. Rd.
Taipei, 10449
Taiwan

Mackay Medical College - Department of Medicine ( email )

Taipei, 252
Taiwan

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