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The Degradation and Performance of Electrospun Supramolecular Vascular Scaffolds Examined Upon In Vitro Enzymatic Exposure

43 Pages Posted: 30 Jan 2019 First Look: Accepted

See all articles by E.E. van Haaftena

E.E. van Haaftena

Eindhoven University of Technology (TUE) - Department of Biomedical Engineering; Eindhoven University of Technology (TUE) - Institute for Complex Molecular Systems (ICMS)

R. Duijvelshoff

Eindhoven University of Technology (TUE) - Department of Biomedical Engineering; Eindhoven University of Technology (TUE) - Institute for Complex Molecular Systems (ICMS)

B.D. Ippela

Eindhoven University of Technology (TUE) - Department of Biomedical Engineering; Eindhoven University of Technology (TUE) - Institute for Complex Molecular Systems (ICMS)

S.H.M. Söntjens

SyMO-Chem B.V.

M.H.C.J. van Houtem

SyMO-Chem B.V.

H.M. Janssen

SyMO-Chem B.V.

A.I.P.M. Smits

Eindhoven University of Technology (TUE) - Department of Biomedical Engineering; Eindhoven University of Technology (TUE) - Institute for Complex Molecular Systems (ICMS)

N.A. Kurniawan

Eindhoven University of Technology (TUE) - Department of Biomedical Engineering; Eindhoven University of Technology (TUE) - Institute for Complex Molecular Systems (ICMS)

P.Y.W. Dankers

Eindhoven University of Technology (TUE) - Department of Biomedical Engineering; Eindhoven University of Technology (TUE) - Institute for Complex Molecular Systems (ICMS)

C.V.C. Bouten

Eindhoven University of Technology (TUE) - Department of Biomedical Engineering; Eindhoven University of Technology (TUE) - Institute for Complex Molecular Systems (ICMS)

Abstract

To maintain functionality during in situ regeneration of load-bearing tissues, a balance between the rates of implant degradation and neo-tissue formation is required. Strictly segmented thermoplastic elastomers with supramolecularly interacting bis-urea (BU) hard blocks are attractive biomaterials for vascular tissue engineering, as these polymers are soft, tough, and biodegradable. Moreover, these materials possess a sequence-controlled macromolecular structure, so their susceptibility to degradation is tunable by controlling the nature of the polymer backbone. It is unknown, however, how the implant’s functionality is affected by the degradation of the polymers it is composed of. We therefore examined the macro- and microscopic features as well as the mechanical performance of vascular scaffolds upon in vitro enzymatic degradation. Three candidate biomaterials (‘slow-degrading’ polycarbonate-BU (PC-BU), ‘intermediate-degrading’ polycarbonate-ester-BU (PC(e)-BU), and ‘fast-degrading’ polycaprolactone-ester-BU (PCL-BU)) were synthesized and electrospun into microporous scaffolds. The scaffolds were incubated in lipase and monitored for changes in physical, chemical, and mechanical properties. Remarkably, comparing PC-BU to PC(e)-BU, we observed that small changes in macromolecular structure led to significant differences in degradation kinetics. All three scaffold types degraded via surface erosion, which was accompanied by fiber swelling for PC-BU scaffolds, and some bulk degradation and a collapsing network for PCL-BU scaffolds. For the PC-BU and PC(e)-BU scaffolds this resulted in retention of mechanical properties, whereas for the PCL-BU scaffolds this resulted in stiffening. Our in vitro study demonstrates that vascular scaffolds, electrospun from sequence-controlled supramolecular materials with varying ester contents, not only display different susceptibilities to degradation, but also degrade via different mechanisms.

Keywords: tissue engineering, vascular graft, electrospinning, lipase, bulk and surface erosion

Suggested Citation

Haaftena, E.E. van and Duijvelshoff, R. and Ippela, B.D. and Söntjens, S.H.M. and Houtem, M.H.C.J. van and Janssen, H.M. and Smits, A.I.P.M. and Kurniawan, N.A. and Dankers, P.Y.W. and Bouten, C.V.C., The Degradation and Performance of Electrospun Supramolecular Vascular Scaffolds Examined Upon In Vitro Enzymatic Exposure (January 29, 2019). Available at SSRN: https://ssrn.com/abstract=3325399

E.E. van Haaftena (Contact Author)

Eindhoven University of Technology (TUE) - Department of Biomedical Engineering

PO Box 513
Eindhoven, 5600 MB
Netherlands

Eindhoven University of Technology (TUE) - Institute for Complex Molecular Systems (ICMS)

PO Box 513
Eindhoven, 5600 MB
Netherlands

R. Duijvelshoff

Eindhoven University of Technology (TUE) - Department of Biomedical Engineering

PO Box 513
Eindhoven, 5600 MB
Netherlands

Eindhoven University of Technology (TUE) - Institute for Complex Molecular Systems (ICMS)

PO Box 513
Eindhoven, 5600 MB
Netherlands

B.D. Ippela

Eindhoven University of Technology (TUE) - Department of Biomedical Engineering

PO Box 513
Eindhoven, 5600 MB
Netherlands

Eindhoven University of Technology (TUE) - Institute for Complex Molecular Systems (ICMS)

PO Box 513
Eindhoven, 5600 MB
Netherlands

S.H.M. Söntjens

SyMO-Chem B.V.

Den Dolech 2
5612 AZ Eindhoven
Netherlands

M.H.C.J. van Houtem

SyMO-Chem B.V.

Den Dolech 2
5612 AZ Eindhoven
Netherlands

H.M. Janssen

SyMO-Chem B.V.

Den Dolech 2
5612 AZ Eindhoven
Netherlands

A.I.P.M. Smits

Eindhoven University of Technology (TUE) - Department of Biomedical Engineering

PO Box 513
Eindhoven, 5600 MB
Netherlands

Eindhoven University of Technology (TUE) - Institute for Complex Molecular Systems (ICMS)

PO Box 513
Eindhoven, 5600 MB
Netherlands

N.A. Kurniawan

Eindhoven University of Technology (TUE) - Department of Biomedical Engineering

PO Box 513
Eindhoven, 5600 MB
Netherlands

Eindhoven University of Technology (TUE) - Institute for Complex Molecular Systems (ICMS)

PO Box 513
Eindhoven, 5600 MB
Netherlands

P.Y.W. Dankers

Eindhoven University of Technology (TUE) - Department of Biomedical Engineering

PO Box 513
Eindhoven, 5600 MB
Netherlands

Eindhoven University of Technology (TUE) - Institute for Complex Molecular Systems (ICMS)

PO Box 513
Eindhoven, 5600 MB
Netherlands

C.V.C. Bouten

Eindhoven University of Technology (TUE) - Department of Biomedical Engineering ( email )

PO Box 513
Eindhoven, 5600 MB
Netherlands

Eindhoven University of Technology (TUE) - Institute for Complex Molecular Systems (ICMS) ( email )

PO Box 513
Eindhoven, 5600 MB
Netherlands

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