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Non-Coding Elements Can Influence Ribosomal Protein Functions and the Composition of Assembled Ribosomes

29 Pages Posted: 7 Feb 2019 Sneak Peek Status: Review Complete

See all articles by Na Dong

Na Dong

Nanchang University - Department of Biotechnology

Xiaogang Peng

Nanchang University - Department of Biotechnology; Nanchang University - Jiangxi Province Key Laboratory of Molecular Medicine

Xian Luo

Nanchang University, Institute of Life Science, Department of Biotechnology

Peilin Yu

Nanchang University, Institute of Life Science, Department of Biotechnology; Nanchang University - State Key Laboratory of Food Science and Technology

Rosemary K. Clyne

Queen Mary University of London - School of Biological and Chemical Sciences

Gwo-Jiunn Hwang

Nanchang University - State Key Laboratory of Food Science and Technology; Nanchang University, Institute of Life Science, Department of Biotechnology

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Abstract

“Ribosome code” hypothesis proposes ribosomes composed of different ribosomal protein paralogs might govern distinct functions. Identification of the regulatory elements likely involved in controlling ribosomal protein functions and the distinct composition of the correspondingly assembled paralog-specific ribosomes should be informative to this phenomenon. Here we show that modulation of different RPL36 non-coding elements can influence protein functions independently of the protein abundance. This is manifested by distinct growth rate, differential biogenesis of the 60S ribosomal subunits, and characteristic composition of the purified translating ribosomes. Proteomic analysis of highly purified paralog-specific ribosomes from strain co-expressing identical Arabidopsis RPL36B proteins from two different constructs reveals their unique composition as two distinctive classes of ribosomes characterized by specifically-enriched components. This observation is remarkably analogous to the composition of endogenous RPL36 paralog-specific ribosomes. The proposed mechanism underlying functional distinction between ribosomal protein paralogs and the significance of evolving paralog-specific ribosomes are presented.

Suggested Citation

Dong, Na and Peng, Xiaogang and Luo, Xian and Yu, Peilin and Clyne, Rosemary K. and Hwang, Gwo-Jiunn, Non-Coding Elements Can Influence Ribosomal Protein Functions and the Composition of Assembled Ribosomes (February 7, 2019). Available at SSRN: https://ssrn.com/abstract=3330560 or http://dx.doi.org/10.2139/ssrn.3330560
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Na Dong

Nanchang University - Department of Biotechnology

Nanchang
China

Xiaogang Peng

Nanchang University - Department of Biotechnology

Nanchang
China

Nanchang University - Jiangxi Province Key Laboratory of Molecular Medicine

Nanchang, 330006
China

Xian Luo

Nanchang University, Institute of Life Science, Department of Biotechnology

Nanchang
China

Peilin Yu

Nanchang University, Institute of Life Science, Department of Biotechnology

Nanchang
China

Nanchang University - State Key Laboratory of Food Science and Technology

Nanchang, 330031
China

Rosemary K. Clyne

Queen Mary University of London - School of Biological and Chemical Sciences

London
United Kingdom

Gwo-Jiunn Hwang (Contact Author)

Nanchang University - State Key Laboratory of Food Science and Technology ( email )

Nanchang, 330031
China

Nanchang University, Institute of Life Science, Department of Biotechnology ( email )

Nanchang
China

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