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Affirmation of Distinctive Ribosomal Protein Paralog-Specific Ribosomes

34 Pages Posted: 15 Feb 2019 Sneak Peek Status: Review Complete

See all articles by Peilin Yu

Peilin Yu

Nanchang University, Institute of Life Science, Department of Biotechnology; Nanchang University - State Key Laboratory of Food Science and Technology

Shengyi Wang

Nanchang University, Institute of Life Science, Department of Biotechnology

Chengying Ma

Peking University, School of Life Science, Peking-Tsinghua Center for Life Sciences, State Key Laboratory of Membrane Biology

Xian Luo

Nanchang University, Institute of Life Science, Department of Biotechnology

Zhihao Xing

Nanchang University, Institute of Life Science, Department of Biotechnology

Xingui Wu

Nanchang University, Institute of Life Science, Department of Biotechnology

Rosemary K. Clyne

Queen Mary University of London - School of Biological and Chemical Sciences

Gwo-Jiunn Hwang

Nanchang University - State Key Laboratory of Food Science and Technology; Nanchang University, Institute of Life Science, Department of Biotechnology

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Abstract

Accumulating evidences favor the existence of heterogeneous cellular ribosomes for versatile regulation of gene expression. Validation of distinctive ribosomal protein paralog-specific ribosomes in single cells at the protein level in nearly wild type background is essential for detailed understanding regarding the properties and functions of specialized ribosomes. Here we present results from cleanly purified RPL36 paralog-specific translating ribosomes to demonstrate they represent two distinctive populations characterized by specifically-enriched components. Under amino acid starvation, there is a dramatic reorganization in the composition of the two paralog-specific ribosomes together with reciprocal redistribution of the general translational factors and altered translational preference. pADH1-RPL36A manipulation improves the relative incompetency of the RPL36A gene; alters composition of both RPL36 paralog-specific ribosomes; reprograms genome-wide translational profile; and modifies the cellular ability to adapt to stress conditions while still preserving the paralog-specifically enriched components. Distinct folding is suggested to underlie functional differences between the paralogs.

Suggested Citation

Yu, Peilin and Wang, Shengyi and Ma, Chengying and Luo, Xian and Xing, Zhihao and Wu, Xingui and Clyne, Rosemary K. and Hwang, Gwo-Jiunn, Affirmation of Distinctive Ribosomal Protein Paralog-Specific Ribosomes (February 14, 2019). Available at SSRN: https://ssrn.com/abstract=3334430 or http://dx.doi.org/10.2139/ssrn.3334430
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Peilin Yu

Nanchang University, Institute of Life Science, Department of Biotechnology

Nanchang
China

Nanchang University - State Key Laboratory of Food Science and Technology

Nanchang, 330031
China

Shengyi Wang

Nanchang University, Institute of Life Science, Department of Biotechnology

Nanchang
China

Chengying Ma

Peking University, School of Life Science, Peking-Tsinghua Center for Life Sciences, State Key Laboratory of Membrane Biology

Beijing, 100871
China

Xian Luo

Nanchang University, Institute of Life Science, Department of Biotechnology

Nanchang
China

Zhihao Xing

Nanchang University, Institute of Life Science, Department of Biotechnology

Nanchang
China

Xingui Wu

Nanchang University, Institute of Life Science, Department of Biotechnology

Nanchang
China

Rosemary K. Clyne

Queen Mary University of London - School of Biological and Chemical Sciences

London
United Kingdom

Gwo-Jiunn Hwang (Contact Author)

Nanchang University - State Key Laboratory of Food Science and Technology ( email )

Nanchang, 330031
China

Nanchang University, Institute of Life Science, Department of Biotechnology ( email )

Nanchang
China

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