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The Circadian Protein Nocturnin Regulates Metabolic Adaptation in Brown Adipose Tissue

34 Pages Posted: 20 Feb 2019 Sneak Peek Status: Published

See all articles by Yasemin Onder

Yasemin Onder

University of Texas at Dallas - Department of Neuroscience

Isara Laothamatas

University of Texas at Dallas - Department of Neuroscience

Stefano Berto

University of Texas at Dallas - Department of Neuroscience

Katharina Sewart

University of Texas at Dallas - Department of Neuroscience

Gokhul Kilaru

University of Texas at Dallas - Department of Neuroscience

Bogdan Bordieanu

University of Texas at Dallas - Children’s Medical Center Research Institute

Jeremy Stubblefield

UT Health San Antonio - Department of Cell Systems and Anatomy

Genevieve Konopka

University of Texas at Dallas - Department of Neuroscience

Prashant Mishra

University of Texas at Dallas - Children’s Medical Center Research Institute

Carla B. Green

University of Texas at Dallas - Department of Neuroscience

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Abstract

Fine-tuning of transcriptional responses can be critical for long-term outcomes in response to an environmental challenge. The circadian protein Nocturnin belongs to a family of proteins that include exonucleases, endonucleases and phosphatases and is most closely related to the CCR4 family of deadenylases that regulate the cellular transcriptome via control of polyA tail length of RNA transcripts. In this study, we investigate the role of Nocturnin in regulating the transcriptional response and downstream metabolic adaptations during cold exposure in brown adipose tissue. We find that Nocturnin exhibits dual localization within the cytosol and mitochondria, and loss of Nocturnin causes changes in expression of networks of mRNAs involved in mitochondrial function. Furthermore, Nocturnin-null animals display significantly elevated levels of TCA cycle intermediates indicating that they have distinct metabolic adaptations during a prolonged cold exposure. We conclude that Nocturnin’s role in multiple cellular compartments allows for coordinated metabolic response to environmental challenges.

Suggested Citation

Onder, Yasemin and Laothamatas, Isara and Berto, Stefano and Sewart, Katharina and Kilaru, Gokhul and Bordieanu, Bogdan and Stubblefield, Jeremy and Konopka, Genevieve and Mishra, Prashant and Green, Carla B., The Circadian Protein Nocturnin Regulates Metabolic Adaptation in Brown Adipose Tissue (February 15, 2019). Available at SSRN: https://ssrn.com/abstract=3334980 or http://dx.doi.org/10.2139/ssrn.3334980
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Yasemin Onder

University of Texas at Dallas - Department of Neuroscience

United States

Isara Laothamatas

University of Texas at Dallas - Department of Neuroscience

United States

Stefano Berto

University of Texas at Dallas - Department of Neuroscience

United States

Katharina Sewart

University of Texas at Dallas - Department of Neuroscience

United States

Gokhul Kilaru

University of Texas at Dallas - Department of Neuroscience

United States

Bogdan Bordieanu

University of Texas at Dallas - Children’s Medical Center Research Institute

2601 North Floyd Road
Richardson, TX 75083
United States

Jeremy Stubblefield

UT Health San Antonio - Department of Cell Systems and Anatomy

One UTSA Circle
San Antonio, TX 78249
United States

Genevieve Konopka

University of Texas at Dallas - Department of Neuroscience

United States

Prashant Mishra

University of Texas at Dallas - Children’s Medical Center Research Institute

2601 North Floyd Road
Richardson, TX 75083
United States

Carla B. Green (Contact Author)

University of Texas at Dallas - Department of Neuroscience ( email )

United States

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