lancet-header
Preprints with The Lancet is part of SSRN´s First Look, a place where journals and other research experts identify content of interest prior to publication. These preprint papers are not peer-reviewed and are posted here as part of a 12-month trial. Authors have either opted in at submission to The Lancet family of journals to post their preprints on Preprints with The Lancet, or submitted directly via SSRN. The usual SSRN checks and a Lancet-specific check for appropriateness and transparency have been applied. These papers should not be used for clinical decision making or reporting of research to a lay audience without indicating that this is preliminary research that has not been peer-reviewed. For more information see the Comment published in The Lancet, or visit The Lancet´s FAQ page, and for any feedback please contact preprints@lancet.com

Overexpression of Human NPY or AgRP in the Paraventricular Nucleus Increases Feeding and Bodyweight in Young Rhesus Macaques

25 Pages Posted: 20 Feb 2019

See all articles by Tao Zhou

Tao Zhou

ShanghaiTech University - School of Life Science and Technology

Ke Tang

Guangzhou University, School of Life Sciences, Precise Genome Engineering Center

Zhengwu Wang

Sichuan Hengshu Bio-Technology Co., Ltd.

Zunfu Ke

Sun Yat-Sen University (SYSU), Zhongshan School of Medicine, First Affiliated Hospital, Department of Pathology; Sun Yat-Sen University (SYSU) - Institute of Precision Medicine

Fan Huang

Government of the People's Republic of China, People's Liberation Army (PLA), First People’s Hospital of Yibin

Ruizhi Tan

Southwest Medical University, Affiliated Traditional Medicine Hospital, Research Center of Integrated Chinese and Western Medicine

Liufang Yin

Shanghai University of Traditional Chinese Medicine - Drug Discovery Lab

Hongli Li

Shanghai University of Traditional Chinese Medicine - Drug Discovery Lab

Shengjie Fan

Shanghai University of Traditional Chinese Medicine - Drug Discovery Lab

Xu Luo

Sichuan Hengshu Bio-Technology Co., Ltd.

Xujiao Ling

Sichuan Hengshu Bio-Technology Co., Ltd.

Xuliang Zhang

ShanghaiTech University - School of Life Science and Technology

Li Wang

Southwest Medical University, Affiliated Traditional Medicine Hospital, Research Center of Integrated Chinese and Western Medicine

Xudong Zhao

Chinese Academy of Sciences (CAS), Kunming Institute of Zoology, Kunming Primate Research Center

Shubin Hong

Sun Yat-Sen University (SYSU) - Institute of Precision Medicine

Sui Peng

Sun Yat-Sen University (SYSU), First Affiliated Hospital, Clinical Trial Unit; Sun Yat-Sen University (SYSU) - Department of Gastroenterology and Hepatology

Qingchun Tong

University of Texas at Houston, McGovern Medical School, Graduate School of Biological Sciences, Brown Foundation Institute of Molecular Medicine and Program in Neuroscience

Xingxu Huang

ShanghaiTech University - School of Life Science and Technology

Junming Fan

ShanghaiTech University - School of Life Science and Technology

Haipeng Xiao

Sun Yat-Sen University (SYSU) - Institute of Precision Medicine

Cheng Huang

Shanghai University of Traditional Chinese Medicine - Drug Discovery Lab

More...

Abstract

Background: The roles of neuropeptides agouti-related peptide (AgRP) and neuropeptide Y (NPY) in feeding behavior regulation have been extensively studied in rodents. However, the biological functions of these peptides in primates are not fully addressed.  

Methods: Lenti-virus mediated expression of human NPY (hNPY) or human AgRP (hAgRP) in paraventricular nucleus (PVN) of the hypothalamus to establish obesity model in monkeys.  

Findings: The feeding behavior and bodyweight were significantly increased in transgenic Rhesus Macaques. Whereas, the blood level of glucose and insulin, as well as metabolic parameters, including blood levels of triglyceride (TG), total cholesterol (TC), high-density lipoprotein-cholesterol (HDL-c), low-density lipoprotein-cholesterol (LDL-c), non-esterified free fatty acids (NEFA) and leptin remained unchanged after virus injection.  

Interpretation: These results have recapitulated some parts of previous reports in rodents, and suggest that these non-germline genetically modified monkeys may provide new models for studying feeding behaviors and related disease, such as obesity and diabetes.  

Funding Statement: This study was supported by the National Natural Science Foundation of China (81671516 to X. Huang) and National Key R&D Program of China (2016YFC0905901 to X. Huang). Xingxu Huang is sponsored by Startup Foundation of ShanghaiTech University.  

Declaration of Interests: All authors have no competing interests to declare, financial or otherwise.

Ethics Approval Statement: The use and care of animals complied with the guideline of the biomedical research ethics committee at the Shanghai University of Traditional Chinese Medicine. The experimental procedures were approved by the Sichuan Hengshu Bio-Tech Co., Ltd animal care and use committee.

Keywords: monkey; agouti-related peptide; neuropeptide Y; feeding behavior; obesity; hypothalamus

Suggested Citation

Zhou, Tao and Tang, Ke and Wang, Zhengwu and Ke, Zunfu and Huang, Fan and Tan, Ruizhi and Yin, Liufang and Li, Hongli and Fan, Shengjie and Luo, Xu and Ling, Xujiao and Zhang, Xuliang and Wang, Li and Zhao, Xudong and Hong, Shubin and Peng, Sui and Tong, Qingchun and Huang, Xingxu and Fan, Junming and Xiao, Haipeng and Huang, Cheng, Overexpression of Human NPY or AgRP in the Paraventricular Nucleus Increases Feeding and Bodyweight in Young Rhesus Macaques (02/14/2019 00:40:55). Available at SSRN: https://ssrn.com/abstract=3335855

Tao Zhou

ShanghaiTech University - School of Life Science and Technology

100 Haike Road, Zhangjiang Hi-Tech Park, Pudong
Research Building
Shanghai, 201210
China

Ke Tang

Guangzhou University, School of Life Sciences, Precise Genome Engineering Center

Waihuanxi Road 230
Guangzhou
China

Zhengwu Wang

Sichuan Hengshu Bio-Technology Co., Ltd.

Yibin 644000
Sichuan
China

Zunfu Ke

Sun Yat-Sen University (SYSU), Zhongshan School of Medicine, First Affiliated Hospital, Department of Pathology ( email )

Guangzhou, Guangdong
China

Sun Yat-Sen University (SYSU) - Institute of Precision Medicine ( email )

Guangzhou, Guangdong
China

Fan Huang

Government of the People's Republic of China, People's Liberation Army (PLA), First People’s Hospital of Yibin

Yibin 64400
Sichuan
China

Ruizhi Tan

Southwest Medical University, Affiliated Traditional Medicine Hospital, Research Center of Integrated Chinese and Western Medicine

Luzhou 646000
Sichuan
China

Liufang Yin

Shanghai University of Traditional Chinese Medicine - Drug Discovery Lab

Shanghai, 201210
China

Hongli Li

Shanghai University of Traditional Chinese Medicine - Drug Discovery Lab

Shanghai, 201210
China

Shengjie Fan

Shanghai University of Traditional Chinese Medicine - Drug Discovery Lab

Shanghai, 201210
China

Xu Luo

Sichuan Hengshu Bio-Technology Co., Ltd.

Yibin 644000
Sichuan
China

Xujiao Ling

Sichuan Hengshu Bio-Technology Co., Ltd.

Yibin 644000
Sichuan
China

Xuliang Zhang

ShanghaiTech University - School of Life Science and Technology

100 Haike Road, Zhangjiang Hi-Tech Park, Pudong
Research Building
Shanghai, 201210
China

Li Wang

Southwest Medical University, Affiliated Traditional Medicine Hospital, Research Center of Integrated Chinese and Western Medicine

Luzhou 646000
Sichuan
China

Xudong Zhao

Chinese Academy of Sciences (CAS), Kunming Institute of Zoology, Kunming Primate Research Center

No.32 Jiaochang Donglu
Kunming, Yunnan 650223
China

Shubin Hong

Sun Yat-Sen University (SYSU) - Institute of Precision Medicine

Guangzhou, Guangdong
China

Sui Peng

Sun Yat-Sen University (SYSU), First Affiliated Hospital, Clinical Trial Unit ( email )

China

Sun Yat-Sen University (SYSU) - Department of Gastroenterology and Hepatology

China

Qingchun Tong

University of Texas at Houston, McGovern Medical School, Graduate School of Biological Sciences, Brown Foundation Institute of Molecular Medicine and Program in Neuroscience

Houston, TX
United States

Xingxu Huang

ShanghaiTech University - School of Life Science and Technology

100 Haike Road, Zhangjiang Hi-Tech Park, Pudong
Research Building
Shanghai, 201210
China

Junming Fan

ShanghaiTech University - School of Life Science and Technology ( email )

100 Haike Road, Zhangjiang Hi-Tech Park, Pudong
Research Building
Shanghai, 201210
China

Haipeng Xiao

Sun Yat-Sen University (SYSU) - Institute of Precision Medicine ( email )

Guangzhou, Guangdong
China

Cheng Huang (Contact Author)

Shanghai University of Traditional Chinese Medicine - Drug Discovery Lab ( email )

Shanghai, 201210
China

Click here to go to TheLancet.com

Go to TheLancet.com

Paper statistics

Abstract Views
48
Downloads
7