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Ultrasound Stimulations Induce Prolonged Depolarization and Fast Action Potentials in Leech Neurons

24 Pages Posted: 4 Mar 2019 Publication Status: Under Review

See all articles by Francesca Dedola

Francesca Dedola

Scuola Superiore Sant'Anna di Pisa - BioRobotics Institute

Francesco Paolo Ulloa Severino

International School for Advanced Studies - Neuroscience Area

Nicolò Meneghetti

Scuola Superiore Sant'Anna di Pisa - BioRobotics Institute

Théo Lemaire

Ecole Polytechnique Fédérale de Lausanne, School of Engineering, Center for Neuroprosthetics and Institute of Bioengineering, Bertarelli Foundation Chair in Translational NeuroEngineering

Andrea Cafarelli

Scuola Superiore Sant'Anna di Pisa - BioRobotics Institute

Leonardo Ricotti

Scuola Superiore Sant'Anna di Pisa - BioRobotics Institute

Arianna Menciassi

Scuola Superiore Sant'Anna di Pisa - BioRobotics Institute

Annarita Cutrone

Scuola Superiore Sant'Anna di Pisa - BioRobotics Institute

Alberto Mazzoni

Scuola Superiore Sant'Anna di Pisa - BioRobotics Institute

Silvestro Micera

Scuola Superiore Sant'Anna di Pisa - BioRobotics Institute; Ecole Polytechnique Fédérale de Lausanne, School of Engineering, Center for Neuroprosthetics and Institute of Bioengineering, Bertarelli Foundation Chair in Translational NeuroEngineering

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Abstract

Ultrasound stimulation (US) carries the promise of a selective, reversible, and non-invasive modulation of neural activity without the need for genetic manipulation of neural structures. However, the mechanisms of US-induced generation of action potentials (APs) are still unclear. Here we address this issue by analyzing intracellularly recorded responses to controlled delivery of US of leech nociceptive neurons. US induced a depolarization linearly accumulating in time and outlasting the duration of the stimulation. Spiking activity was reliably induced for an optimal US intensity range. Moreover, we found that APs induced by US differ in smaller amplitude and faster repolarization from those induced by electrical (EL) stimulation in the same cell, but keep the same repolarization rate. These results shed light on the mechanism by which spikes are induced by US and pave the way for designing more efficient US patterns.

Suggested Citation

Dedola, Francesca and Severino, Francesco Paolo Ulloa and Meneghetti, Nicolò and Lemaire, Théo and Cafarelli, Andrea and Ricotti, Leonardo and Menciassi, Arianna and Cutrone, Annarita and Mazzoni, Alberto and Micera, Silvestro, Ultrasound Stimulations Induce Prolonged Depolarization and Fast Action Potentials in Leech Neurons. Available at SSRN: https://ssrn.com/abstract=3345552 or http://dx.doi.org/10.2139/ssrn.3345552
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Francesca Dedola

Scuola Superiore Sant'Anna di Pisa - BioRobotics Institute

Pisa
Italy

Francesco Paolo Ulloa Severino

International School for Advanced Studies - Neuroscience Area

Trieste
Italy

Nicolò Meneghetti

Scuola Superiore Sant'Anna di Pisa - BioRobotics Institute

Pisa
Italy

Théo Lemaire

Ecole Polytechnique Fédérale de Lausanne, School of Engineering, Center for Neuroprosthetics and Institute of Bioengineering, Bertarelli Foundation Chair in Translational NeuroEngineering

Lausanne
Switzerland

Andrea Cafarelli

Scuola Superiore Sant'Anna di Pisa - BioRobotics Institute

Pisa
Italy

Leonardo Ricotti

Scuola Superiore Sant'Anna di Pisa - BioRobotics Institute

Pisa
Italy

Arianna Menciassi

Scuola Superiore Sant'Anna di Pisa - BioRobotics Institute

Pisa
Italy

Annarita Cutrone

Scuola Superiore Sant'Anna di Pisa - BioRobotics Institute

Pisa
Italy

Alberto Mazzoni

Scuola Superiore Sant'Anna di Pisa - BioRobotics Institute

Pisa
Italy

Silvestro Micera (Contact Author)

Scuola Superiore Sant'Anna di Pisa - BioRobotics Institute

Pisa
Italy

Ecole Polytechnique Fédérale de Lausanne, School of Engineering, Center for Neuroprosthetics and Institute of Bioengineering, Bertarelli Foundation Chair in Translational NeuroEngineering

Lausanne
Switzerland

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