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Tanshinol Borneol Ester Improves Pressure Overload-Induced Cardiac Hypertrophy Through Mitigating Autophagy

26 Pages Posted: 8 Mar 2019

See all articles by Deliang Shen

Deliang Shen

Zhengzhou University - Department of Cardiology

Dongjian Han

Zhengzhou University

Hongxia Liang

Zhengzhou University

Bo Wang

Zhengzhou University

Peiwen Wang

Zhengzhou University

Zhi Jin

Zhengzhou University

Xinyue Cui

Zhengzhou University

Wei He

Zhengzhou University

Shengna Han

Zhengzhou University

Jinying Zhang

Zhengzhou University

Pu Jia

Northwest University (China)

More...

Abstract

Autophagy plays an important role in pressure overload-induced cardiac hypertrophy. Tanshinol borneol ester (DBZ), which is a combination of Dan Shen abstract and borneol, has been proved to be effective in attenuating various cardiovascular diseases. However, whether the effect of DBZ in attenuating cardiac hypertrophy is through regulation of autophagy is unknown. In this study, we investigated the effect of DBZ both in vitro and in vivo. Neonatal rat ventricular myocytes (NRCMs) were cultured to test the effect of DBZ in vitro, and transverse aortic coarctation (TAC) was performed in rats for in vivo study. Results demonstrated that DBZ reduced the size of heart, genes and proteins expression of hypertrophic markers, ROS generation, cellular stress, remodeling and ameliorated the function of left ventricular. Moreover, autophagy was significantly decreased, indicating that DBZ may improve cardiac hypertrophy by inhibiting autophagy. Finally, our results showed that DBZ regulates autophagy via mTOR/p70S6K and ERK1/2 rather than the p38 pathway. In conclusion, these results suggest that DBZ can attenuate cardiac hypertrophy through inhibiting autophagy via mTOR/p70S6K and ERK1/2 pathway.

Funding Statement: This work was supported by grants from the National Natural Science Foundation of China (81873493 to D.S.), Science and technology innovation personnel of the Education Department of Henan Province (19HASTIT002 to D.S.), Science and Technology Leading Talents of Health Commission of Henan Province (51282 project, 100 to D.S.) 5451 Project of Henan Provincial Commission of Health and Family Planning (2015002 to D.S.). Additional support from the University-College Joint Cultivation Fund of Zheng Zhou University (2016-BSTDJJ-19), Henan Province’s Key Laboratory of Cardiac Injury and Repair.

Declaration of Interests: All authors have no competing interests to declare, financial or otherwise.

Ethics Approval Statement: All experimental protocols were approved by the Animal Research Center of Zheng Zhou University and conformed to the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication No. 8523, revised 1996).

Keywords: Salvia miltiorrhiza, Tanshinol borneol ester, cardiac hypertrophy, autophagy

Suggested Citation

Shen, Deliang and Han, Dongjian and Liang, Hongxia and Wang, Bo and Wang, Peiwen and Jin, Zhi and Cui, Xinyue and He, Wei and Han, Shengna and Zhang, Jinying and Jia, Pu, Tanshinol Borneol Ester Improves Pressure Overload-Induced Cardiac Hypertrophy Through Mitigating Autophagy (March 6, 2019). Available at SSRN: https://ssrn.com/abstract=3347894 or http://dx.doi.org/10.2139/ssrn.3347894

Deliang Shen (Contact Author)

Zhengzhou University - Department of Cardiology ( email )

Henan, 450052
China

Dongjian Han

Zhengzhou University

100 Science Avenue
Zhengzhou, Henan 450001
China

Hongxia Liang

Zhengzhou University

100 Science Avenue
Zhengzhou, Henan 450001
China

Bo Wang

Zhengzhou University

100 Science Avenue
Zhengzhou, Henan 450001
China

Peiwen Wang

Zhengzhou University

100 Science Avenue
Zhengzhou, Henan 450001
China

Zhi Jin

Zhengzhou University

100 Science Avenue
Zhengzhou, Henan 450001
China

Xinyue Cui

Zhengzhou University

100 Science Avenue
Zhengzhou, Henan 450001
China

Wei He

Zhengzhou University

100 Science Avenue
Zhengzhou, Henan 450001
China

Shengna Han

Zhengzhou University

100 Science Avenue
Zhengzhou, Henan 450001
China

Jinying Zhang

Zhengzhou University

100 Science Avenue
Zhengzhou, Henan 450001
China

Pu Jia

Northwest University (China)

229 Taibai North Rd
Beilin
Xian, Shaanxi
China

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