Effect of Stemregenin1 and SB431542 Small Molecules on Ex Vivo Expansion of Umbilical Cord Blood Hematopoietic Stem Cells on Biocompatible Polyethersulfone Nanofiber Scaffolds

29 Pages Posted: 4 Apr 2019

See all articles by Sorush Niknamian

Sorush Niknamian

Board Member of Weston A Price Foundation (WAPF), Washington DC, United States; Liberty University

Date Written: july 10, 2018

Abstract

Cord blood Hematopoietic stem cells (HSCs) with several advantages including low chance of viral contamination and low rate of Graft versus host disease (GVHD) are appropriate candidate for vast medical applications such as transplantation. The main obstacle of cord blood HSCs is the low number cells. To improve ex-vivo expansion of umbilical cord HSCs we introduced a new culture system. Isolated HSCs were seeded in Three-dimensional(3D) on Polyethersulfone(PES) scaffolds and Two-dimensional(2D) culture conditions and treated with SB431542 and Stemregenin1(SR1) small molecules. On the fifth and tenth days the expanded cells in different groups were investigated for expression of specific markers by flow cytometry, expression of some stemness genes by qRT-PCR and colony formation by methocult medium. SR1 molecule significantly increased expansion of CD34+ cells while SB431542 induced more CD34+/38+ cells. Also SB431542 treated cells showed higher colony formation capacity. SR1 increased the expression of c-Myc, HOXB4 and SALL4 while SB431542 seemed to inhibit HOXB4 expression and increase SALL4.Together all, this study introduced a new ex vivo culture setting for further medical application of HSCs. Our data showed simultaneous use of these two small molecules can provide appropriate outcome for HSCs transplantation includes both of engraftment and repopulation.

Keywords: Cord blood, 3D culture, Polyether sulfone, Hematopoietic stem cells, SB431542, Stemregenin1, c-Myc, HOXB4, CXCR4, SALL4

JEL Classification: I10, I19

Suggested Citation

Niknamian, Sorush, Effect of Stemregenin1 and SB431542 Small Molecules on Ex Vivo Expansion of Umbilical Cord Blood Hematopoietic Stem Cells on Biocompatible Polyethersulfone Nanofiber Scaffolds (july 10, 2018). Available at SSRN: https://ssrn.com/abstract=3351508 or http://dx.doi.org/10.2139/ssrn.3351508

Sorush Niknamian (Contact Author)

Board Member of Weston A Price Foundation (WAPF), Washington DC, United States ( email )

NW Washington, DC 20016 USA.
Washington, WA Washington 20016
United States

Liberty University ( email )

1971 University Blvd, Lynchburg, VA 24515
Lynchburg, VA Lynchburg 24515
United States
6125035141 (Phone)

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