Nej1 Interacts with Mre11 to Regulate-Tethering and Dna2 Binding at DNA Double-Strand Breaks
40 Pages Posted: 15 Mar 2019 Sneak Peek Status: PublishedMore...
Non-homologous end-joining (NHEJ) and homologous recombination (HR) are the two major pathways of DNA double strand break (DSB) repair and both are highly conserved from yeast to mammals. Nej1 has a role in DNA end tethering and the Mre11/Rad50/Xrs2 (MRX) complex is important for its recruitment to a DSB. Nej1 and Dna2- Sgs1 interact with the C-terminal end of Mre11, which also includes the region where Rad50 binds. Characterization of Nej1 in two rad50 mutants that alter the structural features of MRX showed that Nej1 inhibits Dna2 interactions with Mre11 and Sgs1. The work support a model whereby Nej1 binding to Mre11 provides a layer of regulation to repair pathway choice at the DSB.
Keywords: DSB repair, NHEJ, Nej1, Mre11-Rad50-Xrs2 (MRX), 5’ resection, end-tethering
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