lancet-header

Preprints with The Lancet is part of SSRN´s First Look, a place where journals identify content of interest prior to publication. Authors have opted in at submission to The Lancet family of journals to post their preprints on Preprints with The Lancet. The usual SSRN checks and a Lancet-specific check for appropriateness and transparency have been applied. Preprints available here are not Lancet publications or necessarily under review with a Lancet journal. These preprints are early stage research papers that have not been peer-reviewed. The findings should not be used for clinical or public health decision making and should not be presented to a lay audience without highlighting that they are preliminary and have not been peer-reviewed. For more information on this collaboration, see the comments published in The Lancet about the trial period, and our decision to make this a permanent offering, or visit The Lancet´s FAQ page, and for any feedback please contact preprints@lancet.com.

Circular RNA has_circ_0004277 Arouses Malignant Phenotype of Hepatocellular Carcinoma Cells and Stimulates EMT of Peripheral Cells Through Cellular Communication

33 Pages Posted: 23 Mar 2019

See all articles by Yang Su

Yang Su

Nanjing Medical University - Department of Hepatobiliary and Pancreatic Surgery; Nanjing Medical University - State Key Laboratory of Reproductive Medicine

Bin Li

Nanjing Medical University - The Affiliated Huaian No.1 People’s Hospital

Yilin Hu

Nantong University - Affiliated Hospital

Chuanrong Zhu

Nanjing Medical University - Department of Hepatobiliary and Pancreatic Surgery

More...

Multiple version iconThere are 2 versions of this paper

Abstract

Background: Circular RNAs (circRNAs) are important in the pathological process of many diseases. From accumulative evidences so far, exosomal circRNAs reflect the physiological status of donor cells and various cell reactions are induced after exosomal circRNAs are captured by recipient cells.

Methods: Here, qRT-PCR was performed for detecting the circ-0004277 expression in hepatocellular carcinoma (HCC) cell lines, tissues and plasma exosomes. The influence of circ-0004277 on the proliferation and migration of hepatocellular carcinoma cells was assessed by Cell Counting Kit-8 assays, 5-ethynyl-2'-deoxyuridine assays and Transwell migration assays as well as tumor formation in nude mice. In addition, TEM was performed to indicate the size and shape of exosomes from HCC cell lines and normal liver cell line HL-7702.

Findings: We found significantly unregulated circ-0004277 in HCC cells, tissues and plasma exosomes relative to normal controls. Meanwhile, overexpressed circ-0004277 enhanced the proliferation and migration of HCC cells in vivo and in vitro. What's more, exosomes from hepatocellular carcinoma cells enhanced circ-0004277 expression of surrounding normal cells and stimulated their proliferation and migration. Furthermore, exosomal circ-0004277 stimulated the EMT of peripheral normal cells.

Interpretation: Hence, our findings suggest that on the one hand circ-0004277 arouses malignant phenotype of HCC cells; on the other hand, exosomal circ-0004277 from HCC cells stimulates EMT of peripheral cells through cellular communication to further promote the invasion of HCC into normal surrounding tissues.

Funding: None.

Declaration of Interest: Authors declare that they have no competing interests.

Ethical Approval: All the subjects singed the written informed and the study protocol received the approval from the Ethics Committee of both Nantong University Affiliated Hospital and The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University.

Keywords: hepatocellular carcinoma; circ-0004277; circRNAs; exosomes

Suggested Citation

Su, Yang and Li, Bin and Hu, Yilin and Zhu, Chuanrong, Circular RNA has_circ_0004277 Arouses Malignant Phenotype of Hepatocellular Carcinoma Cells and Stimulates EMT of Peripheral Cells Through Cellular Communication (March 20, 2019). Available at SSRN: https://ssrn.com/abstract=3356844 or http://dx.doi.org/10.2139/ssrn.3356844

Yang Su (Contact Author)

Nanjing Medical University - Department of Hepatobiliary and Pancreatic Surgery ( email )

Huaian, Jiangsu
China

Nanjing Medical University - State Key Laboratory of Reproductive Medicine ( email )

Nanjing
China

Bin Li

Nanjing Medical University - The Affiliated Huaian No.1 People’s Hospital

Huaian, Jiangsu
China

Yilin Hu

Nantong University - Affiliated Hospital

20 Xisi Rd
Chongchuan Qu, 226001
China

Chuanrong Zhu

Nanjing Medical University - Department of Hepatobiliary and Pancreatic Surgery

Huaian, Jiangsu
China

Click here to go to TheLancet.com

Paper statistics

Abstract Views
218
Downloads
18
PlumX Metrics