header

Lithium-Containing Surface Pre-Reacted Glass Fillers in Reparative Dentin Formation

27 Pages Posted: 28 Mar 2019 First Look: Accepted

See all articles by Manahil Ali

Manahil Ali

Osaka University - Department of Restorative Dentistry and Endodontology

Motoki Okamoto

Osaka University - Department of Restorative Dentistry and Endodontology

Shungo Komichi

Osaka University - Department of Restorative Dentistry and Endodontology

Masakatsu Watanabe

Osaka University - Department of Restorative Dentistry and Endodontology

Hailing Huang

Osaka University - Department of Restorative Dentistry and Endodontology

Yusuke Takahashi

Osaka University - Department of Restorative Dentistry and Endodontology

Mikako Hayashi

Osaka University - Department of Restorative Dentistry and Endodontology

Abstract

Surface pre-reacted glass (S-PRG) fillers are new bioactive molecules used in dental clinic work to fill tooth defects. These fillers release various types of ions and exhibit high biocompatibility, antibacterial capability, reduced plaque accumulation, and enhanced osteoblast differentiation. We previously showed that cement S-PRG fillers could induce tertiary dentin formation in rat models. Previous work also showed that lithium ions can activate the Wnt/β-catenin signaling pathway in vitro and induce dentin formation in pulpotomized teeth in vivo. In the current study, we sought to enhance the effect of S-PRG cement by incorporating LiCl. We show that treatment of human dental pulp stem cells with eluates from S-PRG/LiCl combination cements leads to an upregulation in cell migration, differentiation, and mineralization in vitro. In pulp-capping animal trials, we found that S-PRG/LiCl cements could induce tertiary dentin formation 28-days post-capping. At 7 days post-capping, we identified both β-catenin and Axin2 expression using immunofluorescence, indicative of Wnt/β-catenin signaling activity. In conclusion, S-PRG/LiCl cement is highly effective in promoting human dental pulp stem cells profiles and in enhancing reparative dentin formation in rat teeth through activation of the Wnt/β-catenin canonical signaling pathway.

Keywords: direct pulp capping, dentin regeneration, lithium chloride, S-PRG fillers, Wnt/β-catenin pathway

Suggested Citation

Ali, Manahil and Okamoto, Motoki and Komichi, Shungo and Watanabe, Masakatsu and Huang, Hailing and Takahashi, Yusuke and Hayashi, Mikako, Lithium-Containing Surface Pre-Reacted Glass Fillers in Reparative Dentin Formation (March 23, 2019). Available at SSRN: https://ssrn.com/abstract=3360257

Manahil Ali (Contact Author)

Osaka University - Department of Restorative Dentistry and Endodontology

1-1 Yamadaoka
Suita
Osaka, 565-0871
Japan

Motoki Okamoto

Osaka University - Department of Restorative Dentistry and Endodontology

1-1 Yamadaoka
Suita
Osaka, 565-0871
Japan

Shungo Komichi

Osaka University - Department of Restorative Dentistry and Endodontology

1-1 Yamadaoka
Suita
Osaka, 565-0871
Japan

Masakatsu Watanabe

Osaka University - Department of Restorative Dentistry and Endodontology

1-1 Yamadaoka
Suita
Osaka, 565-0871
Japan

Hailing Huang

Osaka University - Department of Restorative Dentistry and Endodontology

1-1 Yamadaoka
Suita
Osaka, 565-0871
Japan

Yusuke Takahashi

Osaka University - Department of Restorative Dentistry and Endodontology ( email )

1-1 Yamadaoka
Suita
Osaka, 565-0871
Japan

Mikako Hayashi

Osaka University - Department of Restorative Dentistry and Endodontology

1-1 Yamadaoka
Suita
Osaka, 565-0871
Japan

Register to save articles to
your library

Register

Paper statistics

Abstract Views
44
Downloads
7