Spongee: A Genetic Tool for Subcellular and Cell-Specific cGMP Manipulation

Ros et al., 2019, Cell Reports 27, 4003–4012, June 25, 2019. DOI: 10.1016/j.celrep.2019.05.102

32 Pages Posted: 3 May 2019 Last revised: 26 Jun 2019

See all articles by Oriol Ros

Oriol Ros

Sorbonne Université - Institut de la Vision

Solène Ribes

Sorbonne University - Institut de la Vision

Karine Loulier

INSERM, Institut des Neurosciences de Montpellier

Sandrine Couvet

Sorbonne Université - Institut de la Vision

Yvrick Zagar

Sorbonne Université - Institut de la Vision

Delphine Ladarre

ESPCI Paris

Alice Louail

Sorbonne Université - Institut de la Vision

Sarah Baudet

Sorbonne Université - Institut de la Vision

Zsolt Lenkei

INSERM U1266

Xavier Nicol

Sorbonne Université - Institut de la Vision

Date Written: April 1, 2019

Abstract

cGMP is critical to a variety of cellular processes, but the available tools to interfere with endogenous cGMP lack cellular and subcellular specificity. We introduce SponGee, a genetically-encoded chelator of this cyclic nucleotide that enables in vitro and in vivo manipulations in single cells and in biochemically-defined subcellular compartments. SponGee buffers physiological changes in cGMP concentration in various model systems while not affecting cAMP signals. We provide proof of concept strategies using this tool to highlight the role of cGMP signaling in vivo and in discrete subcellular domains. SponGee enables the investigation of local cGMP signals in vivo and paves the way to therapeutic strategies preventing downstream signaling activation.

Keywords: cGMP buffer, subcellular compartment, single cell pharmacology, axon guidance, neuronal migration

Suggested Citation

Ros, Oriol and Ribes, Solène and Loulier, Karine and Couvet, Sandrine and Zagar, Yvrick and Ladarre, Delphine and Louail, Alice and Baudet, Sarah and Lenkei, Zsolt and Nicol, Xavier, Spongee: A Genetic Tool for Subcellular and Cell-Specific cGMP Manipulation (April 1, 2019). Ros et al., 2019, Cell Reports 27, 4003–4012, June 25, 2019. DOI: 10.1016/j.celrep.2019.05.102. Available at SSRN: https://ssrn.com/abstract=3363492 or http://dx.doi.org/10.2139/ssrn.3363492

Oriol Ros

Sorbonne Université - Institut de la Vision ( email )

17 rue Moreau
Paris, 75012
France

Solène Ribes

Sorbonne University - Institut de la Vision ( email )

17 rue Moreau
Paris, 75012
France

Karine Loulier

INSERM, Institut des Neurosciences de Montpellier ( email )

Hôpital Saint Eloi, INM, Inserm U1051
80 avenue Augustin Fliche
Montpellier, 34000
France

Sandrine Couvet

Sorbonne Université - Institut de la Vision ( email )

17 rue Moreau
Paris, 75012
France

Yvrick Zagar

Sorbonne Université - Institut de la Vision ( email )

17 rue Moreau
Paris, 75012
France

Delphine Ladarre

ESPCI Paris ( email )

10 Rue Vauquelin
75005 Paris
France

Alice Louail

Sorbonne Université - Institut de la Vision ( email )

17 rue Moreau
Paris, 75012
France

Sarah Baudet

Sorbonne Université - Institut de la Vision ( email )

17 rue Moreau
Paris, 75012
France

Zsolt Lenkei

INSERM U1266 ( email )

102, rue de la Sante
Paris, 75014
France

Xavier Nicol (Contact Author)

Sorbonne Université - Institut de la Vision ( email )

17 rue Moreau
Paris, 75012
France

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