Type I Interferon Shapes the Quantity and Quality of the Anti-Zika Virus Antibody Response
45 Pages
Posted: 11 Apr 2019
Sneak Peek Status: Review Complete
See all articles by Cheryl Yi-Pin LeeAgency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN)
Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN)
Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN)
Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN)
Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN)
Agency for Science, Technology and Research (A*STAR) - Institute of Molecular and Cell Biology
Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN)
Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN)
Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN)
Agency for Science, Technology and Research (A*STAR) - Singapore Immunology Network (SIgN)
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Abstract
Without susceptible wild-type mouse models, current ZIKV studies rely on type I IFN-deficient animals. Type I IFN signalling is involved in humoral response regulation and thus, assessing type I IFN suppression on B-cell response during ZIKV infection is important. Using serum samples from ZIKV-infected animals that are either Type I IFN competent or transiently suppressed, serological assays revealed more robust antibody response and subtype switching upon type I IFN blockade. This was accompanied by an increase germinal centers, plasma cells and germinal center B cells. Interestingly, although both groups recognized different epitopes in the E and NS1 regions, neutralizing capacity was not altered. Finally, results showed that E protein epitopes are important in controlling ZIKV infection. This study highlights the role of type I IFN in shaping the anti-ZIKV antibody response to generate beneficial antibodies, and will help guide development of vaccines triggering efficient neutralizing antibodies and avoiding detrimental ones.
Keywords: Zika virus, type I interferon, humoral response, antibodies
Suggested Citation:
Suggested Citation
Lee, Cheryl Yi-Pin and Chen, Zheyuan and Carissimo, Guillaume and Lum, Fok-Moon and Bakar, Farhana Abu and Rajarethinam, Ravisankar and Teo, Teck-Hui and Torres-Ruesta, Anthony and Renia, Laurent and Ng, Lisa F.P., Type I Interferon Shapes the Quantity and Quality of the Anti-Zika Virus Antibody Response (April 2, 2019). Available at SSRN:
https://ssrn.com/abstract=3364377 or
http://dx.doi.org/10.2139/ssrn.3364377
This is a paper under consideration at Cell Press and has not been peer-reviewed.