puc-header

Type I Interferon Shapes the Quantity and Quality of the Anti-Zika Virus Antibody Response

45 Pages Posted: 11 Apr 2019 Sneak Peek Status: Under Review

See all articles by Cheryl Yi-Pin Lee

Cheryl Yi-Pin Lee

Agency for Science, Technology and Research - Singapore Immunology Network (SIgN)

Zheyuan Chen

Agency for Science, Technology and Research - Singapore Immunology Network (SIgN)

Guillaume Carissimo

Agency for Science, Technology and Research - Singapore Immunology Network (SIgN)

Fok-Moon Lum

Agency for Science, Technology and Research - Singapore Immunology Network (SIgN)

Farhana Abu Bakar

Agency for Science, Technology and Research - Singapore Immunology Network (SIgN)

Ravisankar Rajarethinam

Agency for Science, Technology and Research - Institute of Molecular and Cell Biology

Teck-Hui Teo

Agency for Science, Technology and Research - Singapore Immunology Network (SIgN)

Anthony Torres-Ruesta

Agency for Science, Technology and Research - Singapore Immunology Network (SIgN)

Laurent Renia

Agency for Science, Technology and Research - Singapore Immunology Network (SIgN)

Lisa F.P. Ng

Agency for Science, Technology and Research - Singapore Immunology Network (SIgN)

More...

Abstract

Without susceptible wild-type mouse models, current ZIKV studies rely on type I IFN-deficient animals. Type I IFN signalling is involved in humoral response regulation and thus, assessing type I IFN suppression on B-cell response during ZIKV infection is important. Using serum samples from ZIKV-infected animals that are either Type I IFN competent or transiently suppressed, serological assays revealed more robust antibody response and subtype switching upon type I IFN blockade. This was accompanied by an increase germinal centers, plasma cells and germinal center B cells. Interestingly, although both groups recognized different epitopes in the E and NS1 regions, neutralizing capacity was not altered. Finally, results showed that E protein epitopes are important in controlling ZIKV infection. This study highlights the role of type I IFN in shaping the anti-ZIKV antibody response to generate beneficial antibodies, and will help guide development of vaccines triggering efficient neutralizing antibodies and avoiding detrimental ones.

Keywords: Zika virus, type I interferon, humoral response, antibodies

Suggested Citation

Lee, Cheryl Yi-Pin and Chen, Zheyuan and Carissimo, Guillaume and Lum, Fok-Moon and Bakar, Farhana Abu and Rajarethinam, Ravisankar and Teo, Teck-Hui and Torres-Ruesta, Anthony and Renia, Laurent and Ng, Lisa F.P., Type I Interferon Shapes the Quantity and Quality of the Anti-Zika Virus Antibody Response (April 2, 2019). Available at SSRN: https://ssrn.com/abstract=3364377 or http://dx.doi.org/10.2139/ssrn.3364377
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Cheryl Yi-Pin Lee

Agency for Science, Technology and Research - Singapore Immunology Network (SIgN)

1 Fusionopolis Way
#16-16 Connexis
Singapore, 138632
Singapore

Zheyuan Chen

Agency for Science, Technology and Research - Singapore Immunology Network (SIgN)

1 Fusionopolis Way
#16-16 Connexis
Singapore, 138632
Singapore

Guillaume Carissimo

Agency for Science, Technology and Research - Singapore Immunology Network (SIgN)

1 Fusionopolis Way
#16-16 Connexis
Singapore, 138632
Singapore

Fok-Moon Lum

Agency for Science, Technology and Research - Singapore Immunology Network (SIgN)

1 Fusionopolis Way
#16-16 Connexis
Singapore, 138632
Singapore

Farhana Abu Bakar

Agency for Science, Technology and Research - Singapore Immunology Network (SIgN)

1 Fusionopolis Way
#16-16 Connexis
Singapore, 138632
Singapore

Ravisankar Rajarethinam

Agency for Science, Technology and Research - Institute of Molecular and Cell Biology

Singapore

Teck-Hui Teo

Agency for Science, Technology and Research - Singapore Immunology Network (SIgN)

1 Fusionopolis Way
#16-16 Connexis
Singapore, 138632
Singapore

Anthony Torres-Ruesta

Agency for Science, Technology and Research - Singapore Immunology Network (SIgN)

1 Fusionopolis Way
#16-16 Connexis
Singapore, 138632
Singapore

Laurent Renia

Agency for Science, Technology and Research - Singapore Immunology Network (SIgN)

1 Fusionopolis Way
#16-16 Connexis
Singapore, 138632
Singapore

Lisa F.P. Ng (Contact Author)

Agency for Science, Technology and Research - Singapore Immunology Network (SIgN) ( email )

1 Fusionopolis Way
#16-16 Connexis
Singapore, 138632
Singapore

Click here to go to Cell.com

Go to Cell.com

Paper statistics

Abstract Views
71
PlumX Metrics
Downloads
6