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A Spatio-Temporal Sequence of Axonal Sensitization to Slits and Semaphorins Orchestrates Commissural Axon Navigation

40 Pages Posted: 8 Apr 2019 Sneak Peek Status: Under Review

See all articles by Aurora Pignata

Aurora Pignata

University of Lyon 1 - NeuroMyoGene Institute, CNRS UMR5310, INSERM U1217

Hugo Ducuing

University of Lyon 1 - NeuroMyoGene Institute, CNRS UMR5310, INSERM U1217

Leila Boubakar

University of Lyon 1 - NeuroMyoGene Institute, CNRS UMR5310, INSERM U1217

Thibault Gardette

University of Lyon 1 - NeuroMyoGene Institute, CNRS UMR5310, INSERM U1217

Karine Kindbeiter

University of Lyon 1 - NeuroMyoGene Institute, CNRS UMR5310, INSERM U1217

Muriel Bozon

University of Lyon 1 - NeuroMyoGene Institute, CNRS UMR5310, INSERM U1217

Servane Tauszig-Delamasure

University of Lyon 1 - NeuroMyoGene Institute, CNRS UMR5310, INSERM U1217

Julien Falk

University of Lyon 1 - NeuroMyoGene Institute, CNRS UMR5310, INSERM U1217

Olivier Thoumine

University of Bordeaux - Interdisciplinary Institute for Neuroscience, UMR CNRS 5297

Valérie Castellani

University of Lyon 1 - NeuroMyoGene Institute, CNRS UMR5310, INSERM U1217

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Abstract

Accurate perception of guidance cues is crucial for cell and axon migration. During initial navigation in the spinal cord, commissural axons are kept insensitive to midline repellents. Upon midline crossing in the floor plate, they switch on responsiveness to Slit and Semaphorin repulsive signals and are propelled away and prevented from crossing back. Whether and if so how the different midline repellents control specific aspects of the navigation remain puzzling. We set-up a paradigm for live imaging and super resolution analysis of PlexinA1, Neuropilin- 2 and Robo1/2 receptor dynamics during commissural growth cone navigation in chick and mouse embryos. We uncovered a remarkable program of sensitization to midline cues achieved by unique spatio-temporal sequences of receptor allocation at the growth cone surface, eliciting receptor-specific growth cone behavior changes. This reveals post-translational mechanisms whereby coincident guidance signals are temporally resolved, which thus allow the generation of peculiar guidance responses.

Keywords: axon guidance, guidance receptors, Roundabout1/2, PlexinA1, Neuropilin2, spinal cord commissure, midline spinal cord commissure, FRAP live imaging

Suggested Citation

Pignata, Aurora and Ducuing, Hugo and Boubakar, Leila and Gardette, Thibault and Kindbeiter, Karine and Bozon, Muriel and Tauszig-Delamasure, Servane and Falk, Julien and Thoumine, Olivier and Castellani, Valérie, A Spatio-Temporal Sequence of Axonal Sensitization to Slits and Semaphorins Orchestrates Commissural Axon Navigation (April 4, 2019). Available at SSRN: https://ssrn.com/abstract=3366538 or http://dx.doi.org/10.2139/ssrn.3366538
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Aurora Pignata

University of Lyon 1 - NeuroMyoGene Institute, CNRS UMR5310, INSERM U1217

Lyon
France

Hugo Ducuing

University of Lyon 1 - NeuroMyoGene Institute, CNRS UMR5310, INSERM U1217

Lyon
France

Leila Boubakar

University of Lyon 1 - NeuroMyoGene Institute, CNRS UMR5310, INSERM U1217

Lyon
France

Thibault Gardette

University of Lyon 1 - NeuroMyoGene Institute, CNRS UMR5310, INSERM U1217

Lyon
France

Karine Kindbeiter

University of Lyon 1 - NeuroMyoGene Institute, CNRS UMR5310, INSERM U1217

Lyon
France

Muriel Bozon

University of Lyon 1 - NeuroMyoGene Institute, CNRS UMR5310, INSERM U1217

Lyon
France

Servane Tauszig-Delamasure

University of Lyon 1 - NeuroMyoGene Institute, CNRS UMR5310, INSERM U1217

Lyon
France

Julien Falk

University of Lyon 1 - NeuroMyoGene Institute, CNRS UMR5310, INSERM U1217

Lyon
France

Olivier Thoumine

University of Bordeaux - Interdisciplinary Institute for Neuroscience, UMR CNRS 5297

Bordeaux
France

Valérie Castellani (Contact Author)

University of Lyon 1 - NeuroMyoGene Institute, CNRS UMR5310, INSERM U1217 ( email )

Lyon
France

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