Chinese Olive ( Canarium album L.) Fruit Regulates Glucose Utilization by Activating Amp-Activated Protein Kinase
31 Pages Posted: 26 Apr 2019More...
Background: A growing body of evidence demonstrates obesity-induced insulin resistance is considered to promote the development of metabolic diseases. This study was designed to investigate ethyl acetate fraction of Chinese olive fruit extract (CO-EtOAc) mediated attenuation of obesity and hyperglycemia in a mouse model.
Methods: 60% of HFD-fed mice were treated intragastrically with CO-EtOAc for at last six weeks, and body weight, blood biochemical parameters as well as hepatic inflammation response were investigated. On the other hands, we also investigated the molecular mechanism involved in glucose homeostasis and cellular energy balance using L6 myotubes.
Findings: Our results showed that CO-EtOAc treatment significantly reduced the formation of hepatic lipid droplets, body weight gain, blood glucose and improved serum biochemical parameters in HFD induced obese and insulin resistant mice. We further explored the molecular mechanism underlying the blood glucose modulating the effect of CO-EtOAc using L6 myotubes model. We concluded that CO-EtOAc effectively increased glycogen content and glucose uptake by stimulating the membrane translocation of glucose transporter 4. In addition, CO-EtOAc depolarized the mitochondrial membrane potential and decreased mitochondrial oxygen consumption, which may result in AMPK activation and the consequent mitochondrial fission.
Interpretation: This study shows that CO-EtOAc prevents the development of obesity in mice fed with HFD and is also capable of stimulating glucose uptake. The possible mechanism might be due to the effects of CO-EtOAc on activation of AMPK and promotion of mitochondrial fission.
Funding: This work was supported by grants 36 from the Ministry of Science and Technology (MOST 104‐3011‐B‐010‐001‐1‐4‐2, MOST 104‐2320‐B‐002‐039), Taiwan.
Declaration of Interest: The authors declare no competing financial interests.
Ethical Approval: All study protocols were approved by the Institutional Animal Care and Use Committee of National Yang-Ming University. In addition, all experiments were performed in accordance with relevant guidelines and regulations.
Keywords: Chinese olive (Canarium album L.); high-fat diet; AMPK; glucose uptake; mitochondrial
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