lancet-header
Preprints with The Lancet is part of SSRN´s First Look, a place where journals and other research experts identify content of interest prior to publication. These preprint papers are not peer-reviewed. Authors have either opted in at submission to The Lancet family of journals to post their preprints on Preprints with The Lancet, or submitted directly via SSRN. The usual SSRN checks and a Lancet-specific check for appropriateness and transparency have been applied. These papers should not be used for clinical decision making or reporting of research to a lay audience without indicating that this is preliminary research that has not been peer-reviewed. For more information see the Comment published in The Lancet, or visit The Lancet´s FAQ page, and for any feedback please contact preprints@lancet.com

Changing Expression Profiles of lncRNAs During Ischemia-Reperfusion-Induced Renal Fibrosis

36 Pages Posted: 29 Apr 2019

See all articles by Hongtao Chen

Hongtao Chen

Guangzhou Medical University - Guangzhou Eighth People's Hospital

Youling Fan

Panyu Central Hospital

Huan Jing

Government of the People's Republic of China - Department of Anesthesiology

Simin Tang

Government of the People's Republic of China - Department of Anesthesiology

Zhenxing Huang

Government of the People's Republic of China - Department of Anesthesiology

Meijuan Liao

Government of the People's Republic of China - Department of Anesthesiology

Sen Lin

Government of the People's Republic of China - Department of Anesthesiology

Jiying Zhong

Government of the People's Republic of China - Department of Anesthesiology

Jun Zhou

Government of the People's Republic of China - Department of Anesthesiology

More...

Abstract

The pathological physiology of end-stage renal disease is characterized by renal fibrosis, for which suitable therapeutics have not yet been developed. Early tubular damage in ischemia-reperfusion (IR) injury induced acute kidney injury (AKI) is highly relevant to later fibrosis, but the mechanism remains unclear. Moreover, more and more evidence has proven that long non-coding ribonucleic acids (lncRNA) regulate gene expression associated with renal fibrosis. Therefore, we analyzed lncRNA profiles in the kidneys of mice using RNA sequencing during the pathogenesis of IR-induced renal fibrosis. Our results showed that expression levels of 43 lncRNAs and 141 lncRNAs were respectively significantly changed 7 days and 2 weeks after IR treatment. We predicted the functions of the differentially expressed (DE) lncRNAs and target genes with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Interaction networks of lncRNAs, microRNAs (miRNAs) and mRNA were constructed based on the correlation analysis of the DE genes. Based on these informations, we further verified lncRNA (Gm12840) play competing endogenous RNA (ceRNA) mechanism with miR-677-5p to meidiate TGF-β1 induced fibroblast activation via Wnt-induced secreted protein-1 (WISP1)/ protein kinase B (Akt) signaling pathway. Our findings identify that lncRNAs are involved in IR-induced late fibrosis of kidney and may represent a systematic perspective on the important function of IR-induced renal fibrosis as well as new therapeutic targets.

Funding Statement: This work was supported by grants from National Natural Science Foundation of China (81860130), Natural Science Foundation of Guangdong province (2016A030313376), Guangdong Province Science and Technology Planning Project (2017B020247035, 2014A020212612).

Declaration of Interests: All authors declare that they have no competing interests.

Ethics Approval Statement: All experiments using animals were approved by the Institutional Animal Care and Use Committee of the First People hospital of Foshan and breed as approved by the Institutional Animal Care protocol.

Keywords: lncRNA; renal; ischemia reperfusion; fibrosis

Suggested Citation

Chen, Hongtao and Fan, Youling and Jing, Huan and Tang, Simin and Huang, Zhenxing and Liao, Meijuan and Lin, Sen and Zhong, Jiying and Zhou, Jun, Changing Expression Profiles of lncRNAs During Ischemia-Reperfusion-Induced Renal Fibrosis (April 24, 2019). Available at SSRN: https://ssrn.com/abstract=3378674 or http://dx.doi.org/10.2139/ssrn.3378674

Hongtao Chen

Guangzhou Medical University - Guangzhou Eighth People's Hospital

Guangzhou, 510080
China

Youling Fan

Panyu Central Hospital

Guangzhou, 511400
China

Huan Jing

Government of the People's Republic of China - Department of Anesthesiology

Foshan
China

Simin Tang

Government of the People's Republic of China - Department of Anesthesiology

Foshan
China

Zhenxing Huang

Government of the People's Republic of China - Department of Anesthesiology

Foshan
China

Meijuan Liao

Government of the People's Republic of China - Department of Anesthesiology

Foshan
China

Sen Lin

Government of the People's Republic of China - Department of Anesthesiology

Foshan
China

Jiying Zhong

Government of the People's Republic of China - Department of Anesthesiology

Foshan
China

Jun Zhou (Contact Author)

Government of the People's Republic of China - Department of Anesthesiology ( email )

Foshan
China

Click here to go to TheLancet.com

Paper statistics

Abstract Views
164
Downloads
25