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Visualization of a Distributed Synaptic Memory Code in the Drosophila Brain

61 Pages Posted: 3 May 2019 Publication Status: Published

See all articles by Florian Bilz

Florian Bilz

University of Goettingen (Gottingen), Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Department of Molecular Neurobiology of Behavior

Bart Geurten

University of Goettingen (Gottingen), Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Department of Cellular Neurobiology

André Fiala

University of Goettingen (Gottingen), Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Department of Cellular Neurobiology

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Abstract

During associative conditioning animals learn which sensory cues are predictive for positive or negative conditions. Because neurons that encode sensory cues are sparsely distributed across neuronal assemblies, it is difficult to comprehensively monitor the synaptic plasticity that underlies learning and memory formation. One can either observe individual synapses, or large numbers of neurons without synaptic resolution. To combine both aspects we focused on synaptic boutons of Kenyon cells of the Drosophila mushroom body γ-lobe, a brain structure that mediates olfactory learning. A fluorescent Ca2+ sensor was stochastically expressed in single Kenyon cells so that axonal boutons could be assigned to distinct cells, and Ca2+ could be measured across many animals. We show that odor-evoked Ca2+ dynamics across boutons decorrelate as a result of associative learning. These data reveal a synaptic rather than cellular distribution of a memory code and coherence of distributed synaptic activity as a memory-encoding parameter.

Keywords: Drosophila melanogaster, learning and memor, insect brain, Kenyon cell, mushroom body, odor representation, olfactory coding, optical calcium imaging, synaptic plasticity, Neuronal assemblies

Suggested Citation

Bilz, Florian and Geurten, Bart and Fiala, André, Visualization of a Distributed Synaptic Memory Code in the Drosophila Brain (April 30, 2019). Available at SSRN: https://ssrn.com/abstract=3379968 or http://dx.doi.org/10.2139/ssrn.3379968
This version of the paper has not been formally peer reviewed.

Florian Bilz

University of Goettingen (Gottingen), Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Department of Molecular Neurobiology of Behavior ( email )

Gottingen
Germany

Bart Geurten

University of Goettingen (Gottingen), Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Department of Cellular Neurobiology ( email )

Gottingen
Germany

André Fiala (Contact Author)

University of Goettingen (Gottingen), Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Department of Cellular Neurobiology ( email )

Gottingen
Germany

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