puc-header

A Novel Conversion of Roots into Organs with Shoot Stem Characteristics by Inducing Two Transcription Factors

49 Pages Posted: 17 May 2019 Sneak Peek Status: Review Complete

See all articles by Shigeru Hanano

Shigeru Hanano

Kazusa DNA Research Institute

Hajime Tomatsu

Kazusa DNA Research Institute

Ai Ohnishi

University of Tokyo - Graduate School of Arts and Sciences

Koichi Kobayashi

University of Tokyo - Graduate School of Arts and Sciences

Yuki Kondo

University of Tokyo, Graduate School of Life Sciences, Department of Biological Sciences

Shigeyuki Betsuyaku

University of Tokyo, Graduate School of Science, Department of Biological Sciences; Japan Science and Technology Agency (JST)

Eiji Takita

Kazusa DNA Research Institute

Yoshiyuki Ogata

Kazusa DNA Research Institute

Keishi Ozawa

Kazusa DNA Research Institute

Kunihiro Suda

Kazusa DNA Research Institute

Tsutomu Hosouchi

Kazusa DNA Research Institute

Takahiro Nagase

Kazusa DNA Research Institute; Tohoku University, Graduate School of Life Sciences, The Kisarazu Laboratory

Hideyuki Suzuki

Kazusa DNA Research Institute

Nozomu Sakurai

Kazusa DNA Research Institute

Hiroo Fukuda

Japan Science and Technology Agency (JST)

Daisuke Shibata

Kazusa DNA Research Institute; Tohoku University, Graduate School of Life Sciences, The Kisarazu Laboratory

More...

Abstract

Somatic plant cells can regenerate shoots and/or roots or adventitious embryonic calluses, which may induce organ formation under certain conditions. Such regenerations occur via dedifferentiation of somatic cells, induction of organs, and their subsequent outgrowth. Despite recent advances in understanding of plant regeneration, many details of shoot induction remain unclear. Here, we artificially induced shoot stem-like green organs (SSOs) in Arabidopsis thaliana roots via simultaneous induction of two transcription factors (TFs), ARABIDOPSIS THALIANA HOMEOBOX PROTEIN 25 (ATHB25, At5g65410) and the B3 family transcription factor REPRODUCTIVE MERISTEM 7 (REM7, At3g18960), which are expressed in shoot apical meristems under normal growth conditions. The SSOs exhibited negative gravitropism and differentiated vascular bundle phenotypes, strongly suggesting the ectopic appearance of shoot stem characteristics in roots. In the SSOs, shoot-specific and root-specific genes were up- and down-regulated, respectively. These TFs can thus induce the expression of genes controlling shoot stem characteristics and disrupt the maintenance of root meristems by ectopic expression in roots. SSO induction appears to occur without development of an embryonic callus in the main root tip or the primordia of lateral roots, suggesting that these TFs alter the fate of root initiation to induce shoot stem characteristics. Our findings thus provide new insights in the development and regeneration of plant shoot stems.

Keywords: Shoot development, organ growth, transcription factor, synthetic biology, plant, Arabidopsis thaliana

Suggested Citation

Hanano, Shigeru and Tomatsu, Hajime and Ohnishi, Ai and Kobayashi, Koichi and Kondo, Yuki and Betsuyaku, Shigeyuki and Takita, Eiji and Ogata, Yoshiyuki and Ozawa, Keishi and Suda, Kunihiro and Hosouchi, Tsutomu and Nagase, Takahiro and Suzuki, Hideyuki and Sakurai, Nozomu and Fukuda, Hiroo and Shibata, Daisuke, A Novel Conversion of Roots into Organs with Shoot Stem Characteristics by Inducing Two Transcription Factors (May 14, 2019). ISCIENCE-D-19-00402. Available at SSRN: https://ssrn.com/abstract=3387683 or http://dx.doi.org/10.2139/ssrn.3387683
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Shigeru Hanano (Contact Author)

Kazusa DNA Research Institute ( email )

Chiba
Japan

Hajime Tomatsu

Kazusa DNA Research Institute ( email )

Chiba
Japan

Ai Ohnishi

University of Tokyo - Graduate School of Arts and Sciences ( email )

3-8-1 Komaba
Meguro-ku
Tokyo, Tokyo 153-8902
Japan

Koichi Kobayashi

University of Tokyo - Graduate School of Arts and Sciences ( email )

3-8-1 Komaba
Meguro-ku
Tokyo, Tokyo 153-8902
Japan

Yuki Kondo

University of Tokyo, Graduate School of Life Sciences, Department of Biological Sciences ( email )

Tokyo
Japan

Shigeyuki Betsuyaku

University of Tokyo, Graduate School of Science, Department of Biological Sciences ( email )

Tokyo
Japan

Japan Science and Technology Agency (JST) ( email )

Kawaguchi Center Building
4-1-8, Honcho, Kawaguchi-shi
Saitama, 332-0012
Japan

Eiji Takita

Kazusa DNA Research Institute ( email )

Chiba
Japan

Yoshiyuki Ogata

Kazusa DNA Research Institute ( email )

Chiba
Japan

Keishi Ozawa

Kazusa DNA Research Institute ( email )

Chiba
Japan

Kunihiro Suda

Kazusa DNA Research Institute ( email )

Chiba
Japan

Tsutomu Hosouchi

Kazusa DNA Research Institute ( email )

Chiba
Japan

Takahiro Nagase

Kazusa DNA Research Institute ( email )

Chiba
Japan

Tohoku University, Graduate School of Life Sciences, The Kisarazu Laboratory ( email )

Chiba
Japan

Hideyuki Suzuki

Kazusa DNA Research Institute ( email )

Chiba
Japan

Nozomu Sakurai

Kazusa DNA Research Institute ( email )

Chiba
Japan

Hiroo Fukuda

Japan Science and Technology Agency (JST) ( email )

Kawaguchi Center Building
4-1-8, Honcho, Kawaguchi-shi
Saitama, 332-0012
Japan

Daisuke Shibata

Kazusa DNA Research Institute ( email )

Chiba
Japan

Tohoku University, Graduate School of Life Sciences, The Kisarazu Laboratory ( email )

Chiba
Japan

Click here to go to Cell.com

Go to Cell.com

Paper statistics

Abstract Views
278
Downloads
8