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Early Uncoating of COPII from ER Exit Sites Membranes During Cargo Accumulation and Membrane Fission

28 Pages Posted: 18 May 2019 Sneak Peek Status: Review Complete

See all articles by Olga Shomron

Olga Shomron

Tel Aviv University, Sackler School of Medicine, Department of Pathology

Inbar Nevo-Yassaf

Tel Aviv University, Sackler School of Medicine, Department of Pathology

Tamar Aviad

Tel Aviv University, Sackler School of Medicine, Department of Pathology

Yakey Yaffe

Tel Aviv University, Sackler School of Medicine, Department of Pathology

Eitan Erez Zahavi

Tel Aviv University, Sackler School of Medicine, Department of Physiology and Pharmacology

Anna Dukhovny

Tel Aviv University, Sackler School of Medicine, Department of Clinical Immunology and Microbiology

Eran Perlson

Tel Aviv University, Sackler School of Medicine, Department of Physiology and Pharmacology

Ilya Brodsky

Russian Academy of Sciences (RAS) - Institute of Protein Research

Adva Yeheskel

Tel Aviv University, GSW Faculty of Life Sciences, Bioinformatics Unit

Metsada Pasmanik-Chor

Tel Aviv University, GSW Faculty of Life Sciences, Bioinformatics Unit

Anna Mironov

Fondazione Istituto FIRC di Oncologia Molecolare - IFOM

Galina V. Beznoussenko

Fondazione Istituto FIRC di Oncologia Molecolare - IFOM

Alexander A. Mironov

Fondazione Istituto FIRC di Oncologia Molecolare - IFOM

Ella H. Sklan

Tel Aviv University, Sackler School of Medicine, Department of Clinical Immunology and Microbiology

George H. Patterson

National Institutes of Health (NIH), Section on Biophotonics, NIBIB

Yoji Yonemura

Fritz Lipmann Institute (FLI) - Leibniz Institute for Age Research

Christoph Kaether

Fritz Lipmann Institute (FLI) - Leibniz Institute for Age Research

Koret Hirschberg

Tel Aviv University, Sackler School of Medicine, Department of Pathology

More...

Abstract

COPII and COPI are considered to be analogous sets of vesicle coat protein hretrocomplexes. Coupled to cargo selection, they mediate the formation of membrane vesicles translocating in opposite directions to different destinations within the secretory pathway. Here, live cell and electron microscopy provided evidence for a different localization and mode of function of the COPII coat during protein export from the endoplasmic reticulum (ER). Pharmaceutical and genetic perturbations of ER-Golgi transport were used to demonstrate that COPII is recruited to membranes defining the boundary of ER-ER Exit Sites (ERES) where it facilitates Golgi-bound selective cargo concentration. Uncoating of COPII membranes precedes cargo accumulation and fission of Golgi-bound carriers. Moreover, we show that anterograde carriers are positive with the COPI complex. These findings change our understanding of the role of coat proteins in ER to Golgi transport.

Keywords: COPII, ER export, ER exit sites, Secretory pathway, ER-Golgi transport

Suggested Citation

Shomron, Olga and Nevo-Yassaf, Inbar and Aviad, Tamar and Yaffe, Yakey and Zahavi, Eitan Erez and Dukhovny, Anna and Perlson, Eran and Brodsky, Ilya and Yeheskel, Adva and Pasmanik-Chor, Metsada and Mironov, Anna and Beznoussenko, Galina V. and Mironov, Alexander A. and Sklan, Ella H. and Patterson, George H. and Yonemura, Yoji and Kaether, Christoph and Hirschberg, Koret, Early Uncoating of COPII from ER Exit Sites Membranes During Cargo Accumulation and Membrane Fission (May 14, 2019). DEVELOPMENTAL-CELL-D-19-00383. Available at SSRN: https://ssrn.com/abstract=3387686 or http://dx.doi.org/10.2139/ssrn.3387686
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Olga Shomron

Tel Aviv University, Sackler School of Medicine, Department of Pathology ( email )

Tel Aviv
Israel

Inbar Nevo-Yassaf

Tel Aviv University, Sackler School of Medicine, Department of Pathology ( email )

Tel Aviv
Israel

Tamar Aviad

Tel Aviv University, Sackler School of Medicine, Department of Pathology ( email )

Tel Aviv
Israel

Yakey Yaffe

Tel Aviv University, Sackler School of Medicine, Department of Pathology ( email )

Tel Aviv
Israel

Eitan Erez Zahavi

Tel Aviv University, Sackler School of Medicine, Department of Physiology and Pharmacology ( email )

Tel Aviv
Israel

Anna Dukhovny

Tel Aviv University, Sackler School of Medicine, Department of Clinical Immunology and Microbiology ( email )

Tel Aviv
Israel

Eran Perlson

Tel Aviv University, Sackler School of Medicine, Department of Physiology and Pharmacology ( email )

Tel Aviv
Israel

Ilya Brodsky

Russian Academy of Sciences (RAS) - Institute of Protein Research ( email )

Moscow
Russia

Adva Yeheskel

Tel Aviv University, GSW Faculty of Life Sciences, Bioinformatics Unit ( email )

Tel Aviv
Israel

Metsada Pasmanik-Chor

Tel Aviv University, GSW Faculty of Life Sciences, Bioinformatics Unit ( email )

Tel Aviv
Israel

Anna Mironov

Fondazione Istituto FIRC di Oncologia Molecolare - IFOM ( email )

Milan
Italy

Galina V. Beznoussenko

Fondazione Istituto FIRC di Oncologia Molecolare - IFOM ( email )

Milan
Italy

Alexander A. Mironov

Fondazione Istituto FIRC di Oncologia Molecolare - IFOM ( email )

Milan
Italy

Ella H. Sklan

Tel Aviv University, Sackler School of Medicine, Department of Clinical Immunology and Microbiology ( email )

Tel Aviv
Israel

George H. Patterson

National Institutes of Health (NIH), Section on Biophotonics, NIBIB

Bethesda, MD
United States

Yoji Yonemura

Fritz Lipmann Institute (FLI) - Leibniz Institute for Age Research ( email )

Beutenbergstra├če 11
Jena, 07745
Germany

Christoph Kaether (Contact Author)

Fritz Lipmann Institute (FLI) - Leibniz Institute for Age Research ( email )

Beutenbergstra├če 11
Jena, 07745
Germany

Koret Hirschberg

Tel Aviv University, Sackler School of Medicine, Department of Pathology ( email )

Tel Aviv
Israel

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