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Fecal Microbiota As a Non-Invasive Biomarker to Predict the Tissue Iron Accumulation in Intestine Epithelial Cells and Liver

55 Pages Posted: 17 May 2019

See all articles by Bingdong Liu

Bingdong Liu

Chinese Academy of Sciences (CAS) - State Key Laboratory of Applied Microbiology in Southern China

Xiaohan Pan

Chinese Academy of Sciences (CAS) - State Key Laboratory of Applied Microbiology in Southern China

Zhihong Liu

Southern Medical University - Department of Infectious Diseases; Chinese Academy of Sciences (CAS) - State Key Laboratory of Applied Microbiology in Southern China

Shujie Chen

Chinese Academy of Sciences (CAS) - State Key Laboratory of Applied Microbiology in Southern China; Southern Medical University - Department of Infectious Diseases

Mulan Han

Chinese Academy of Sciences (CAS) - State Key Laboratory of Applied Microbiology in Southern China

Guohuan Xu

Chinese Academy of Sciences (CAS) - State Key Laboratory of Applied Microbiology in Southern China

Xiaoshuang Dai

Beijing Genomics Institute (BGI)

Jia Sun

Southern Medical University, Zhujiang Hospital

Jiyang Pan

Jinan University - First Affiliated Hospital

Wei Wang

Jilin University (JLU) - College of Veterinary Medicine; Zhongkai University of Agriculture and Engineering - College of Animal Science & Technology; Capital Medical University - Key Laboratory for Neurodegenerative Disorders of the Ministry of Education

Li Liu

Southern Medical University - Department of Infectious Diseases; Southern Medical University - Hepatology Unit

Liwei Xie

Chinese Academy of Sciences (CAS) - State Key Laboratory of Applied Microbiology in Southern China

More...

Abstract

Iron is an essential trace mineral for the growth, systemic metabolism, and immune response. Dysregulation of iron homeostasis is linked with the development and progression of various diseases. The excessive iron accumulation is highly associated with inflammatory diseases and cancer while iron deficiency leads to growth retardation. Multiple studies have suggested that iron dysbiosis results in alteration of gut microbiota, leading to the disruption of microbial diversity, increase of pathogen abundance and induction of intestinal inflammation. However, screening the widespread studies in the past decades, the association between iron availability and gut microbiota are not completely explored. Furthermore, non-invasive and convenient approach to determine the tissue iron is still limited. In the current study, a murine model for iron dysbiosis was established. 16S rRNA amplicon sequencing and innovated bioinformatic algorithms are utilized to identify key microbiota. Leveraging on these key microbiotas, we established an easily assessible prediction model, which could accurately distinguish the individual under either iron-deprived or iron-fortified condition and precisely predict the tissue iron level of intestine epithelial cells and liver. This could be further applied for early diagnosis of iron dysbiosis-related diseases as a non-invasive approach.

Funding Statement: This work was support by ‘GDAS’ Project of Science and Technology Development (Grant No. 2018GDASCX-0806) to Liwei Xie and supported by National Natural Science Foundation of China (Grant No.: 8187050617) to Jiyang Pan.

Declaration of Interests: The authors declare that there is no conflict of financial or research interest.

Ethics Approval Statement: The animal protocol was proved by the Institute Animal Care Use Committees of GDIM (Permission #: GT-IACUC201704071).

Keywords: Gut microbiota, Iron, RDA, Random forest, LASSO, Ferritin assay, Tissue biopsies

Suggested Citation

Liu, Bingdong and Pan, Xiaohan and Liu, Zhihong and Chen, Shujie and Han, Mulan and Xu, Guohuan and Dai, Xiaoshuang and Sun, Jia and Pan, Jiyang and Wang, Wei and Liu, Li and Xie, Liwei, Fecal Microbiota As a Non-Invasive Biomarker to Predict the Tissue Iron Accumulation in Intestine Epithelial Cells and Liver (May 13, 2019). Available at SSRN: https://ssrn.com/abstract=3388805

Bingdong Liu

Chinese Academy of Sciences (CAS) - State Key Laboratory of Applied Microbiology in Southern China

Guangzhou, 510070
China

Xiaohan Pan

Chinese Academy of Sciences (CAS) - State Key Laboratory of Applied Microbiology in Southern China

Guangzhou, 510070
China

Zhihong Liu

Southern Medical University - Department of Infectious Diseases

Guangdong
China

Chinese Academy of Sciences (CAS) - State Key Laboratory of Applied Microbiology in Southern China

Guangzhou, 510070
China

Shujie Chen

Chinese Academy of Sciences (CAS) - State Key Laboratory of Applied Microbiology in Southern China

Guangzhou, 510070
China

Southern Medical University - Department of Infectious Diseases

Guangdong
China

Mulan Han

Chinese Academy of Sciences (CAS) - State Key Laboratory of Applied Microbiology in Southern China

Guangzhou, 510070
China

Guohuan Xu

Chinese Academy of Sciences (CAS) - State Key Laboratory of Applied Microbiology in Southern China

Guangzhou, 510070
China

Xiaoshuang Dai

Beijing Genomics Institute (BGI)

Shenzhen, Guangdong 518083
China

Jia Sun

Southern Medical University, Zhujiang Hospital

Guangzhou, Guangdong Province
China

Jiyang Pan

Jinan University - First Affiliated Hospital

Guangdong
China

Wei Wang

Jilin University (JLU) - College of Veterinary Medicine ( email )

Guilin Road
Chaoyang
Changchun, Jilin 130021
China

Zhongkai University of Agriculture and Engineering - College of Animal Science & Technology ( email )

China

Capital Medical University - Key Laboratory for Neurodegenerative Disorders of the Ministry of Education ( email )

China

Li Liu

Southern Medical University - Department of Infectious Diseases ( email )

Guangdong
China

Southern Medical University - Hepatology Unit ( email )

Guangdong
China

Liwei Xie (Contact Author)

Chinese Academy of Sciences (CAS) - State Key Laboratory of Applied Microbiology in Southern China ( email )

Guangzhou, 510070
China

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