Comparative Screening of Different Mineral Salt Media for Biosurfactant Production by Halophilic Organisms
Journal of Emerging Technologies and Innovative Research. Vol 6 Issue 5, May-2019. ISSN: 2349-5162.
7 Pages Posted: 11 Jun 2019
Date Written: May 22, 2019
Biosurfactants produced by microorganisms, are amphipathic surface-active molecules containing hydrophilic and hydrophobic moieties that act by emulsifying hydrocarbons increasing their solubilisation and subsequently rendering them available for microbial degradation. A variety of microorganisms are capable of producing biosurfactants, but halophilic organisms deserve special attention as the biosurfactants obtained from halophilic organisms are capable of sustaining in saline, hypersaline and varied conditions of temperature and pH. In this research, an attempt was made to screen different Mineral Salt Media to produce biosurfactant by halophilic organisms. Halophiles are living organisms capable of growth in saline environment. Different versions of Mineral Salt Media such as Mukherjee media, Makkar and Cameotra media, modified indigenous media, inorganic salt media and modified M9 media were studied and modifications were made with respect to carbon and nitrogen sources along with different inducers such as kerosene and diesel. Following inoculation with different halophilic isolates and incubation in different media for one week, it was observed that inorganic salt media, modified indigenous and M9 media proved to give better results of emulsification test as compared to the other media studied. Out of different carbon and nitrogen sources tested, molasses proved to be a relatively better carbon source and ammonium chloride, ammonium nitrate proved to be relatively better nitrogen sources. Inducer such as kerosene enhanced production of biosurfactant.
Keywords: biosurfactant, halophiles, inducer, molasses
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