Lipids and Polymers Mediated CRISPR/Cas9 Gene Editing
35 Pages Posted: 10 Jun 2019
Date Written: June 9, 2019
CRISPR (the clustered, regularly interspaced, short palindromic repeat)/Cas9 nuclease system is the most commonly used method for gene editing, with the advantages of simple operation, high efficiency and high specificity, and has been used in various animals and crops. In this article, we review the latest development in the delivery technology of CRISPR/Cas9 based on lipids and polymers. We discuss the comparative advantages of commercial liposomes, new cationic lipidoid nanoparticles and hybrid liposomes as vectors for the delivery of genes encoding Cas9 and sgRNA, as well as many successful examples of polymer nanoparticles or polymer hybrid nanocarriers. We also introduce research ideas for the construction of non-viral vectors to better modify the structure of lipids and polymers, or to combine several biomaterials in order to better suit the CRISPR/Cas9 delivery. Furthermore, we consider the limitations of the non-viral carriers suitable for the delivery of the CRISPR/Cas9 system, and propose to overcome these obstacles through developing novel gene editing delivery vectors for clinical research.
Keywords: Gene editing, Lipids, Polymers, CRISPR/Cas9
Suggested Citation: Suggested Citation