Lipids and Polymers Mediated CRISPR/Cas9 Gene Editing

35 Pages Posted: 10 Jun 2019

See all articles by Yan Gong

Yan Gong

Dalian Minzu University - College of Life Science

Siyu Tian

Dalian Minzu University - College of Life Science

Shubiao Zhang

Dalian Minzu University - College of Life Science

Date Written: June 9, 2019

Abstract

CRISPR (the clustered, regularly interspaced, short palindromic repeat)/Cas9 nuclease system is the most commonly used method for gene editing, with the advantages of simple operation, high efficiency and high specificity, and has been used in various animals and crops. In this article, we review the latest development in the delivery technology of CRISPR/Cas9 based on lipids and polymers. We discuss the comparative advantages of commercial liposomes, new cationic lipidoid nanoparticles and hybrid liposomes as vectors for the delivery of genes encoding Cas9 and sgRNA, as well as many successful examples of polymer nanoparticles or polymer hybrid nanocarriers. We also introduce research ideas for the construction of non-viral vectors to better modify the structure of lipids and polymers, or to combine several biomaterials in order to better suit the CRISPR/Cas9 delivery. Furthermore, we consider the limitations of the non-viral carriers suitable for the delivery of the CRISPR/Cas9 system, and propose to overcome these obstacles through developing novel gene editing delivery vectors for clinical research.

Keywords: Gene editing, Lipids, Polymers, CRISPR/Cas9

Suggested Citation

Gong, Yan and Tian, Siyu and Zhang, Shubiao, Lipids and Polymers Mediated CRISPR/Cas9 Gene Editing (June 9, 2019). Available at SSRN: https://ssrn.com/abstract=3401354

Yan Gong

Dalian Minzu University - College of Life Science

China

Siyu Tian

Dalian Minzu University - College of Life Science

China

Shubiao Zhang (Contact Author)

Dalian Minzu University - College of Life Science ( email )

China

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