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The Cellular Diversity and Transcription Factor Code of Drosophila Enteroendocrine Cells

57 Pages Posted: 25 Jun 2019 Sneak Peek Status: Published

See all articles by Xingting Guo

Xingting Guo

Tsinghua University - National Institute of Biological Sciences

Chang Yin

Tsinghua University - National Institute of Biological Sciences

Fu Yang

Tsinghua University - National Institute of Biological Sciences

Yongchao Zhang

Tsinghua University - National Institute of Biological Sciences

Huanwei Huang

Tsinghua University - National Institute of Biological Sciences

Jiawen Wang

Tsinghua University - National Institute of Biological Sciences

Bowen Deng

Peking University - School of Life Science

Tao Cai

Tsinghua University - National Institute of Biological Sciences

Yi Rao

Peking University - School of Life Science

Rongwen Xi

Tsinghua University - National Institute of Biological Sciences

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Abstract

Enteroendocrine cells (EEs) in the intestinal epithelium have important endocrine functions, yet this cell lineage exhibits great local and regional variations that have hampered detailed characterization of EE subtypes. Through single cell RNA-sequencing analysis, combined with a collection of neuropeptide/receptor knock-in strains, here we provide a comprehensive analysis of cellular diversity, spatial distribution and transcription factor (TF) code of EEs in adult Drosophila midgut. We identify 10 major EE subtypes that totally produced 14 different classes of hormone peptides. Each EE on average co-produces 2-5 different classes of hormone peptides. Functional screen with subtype-enriched TFs suggests a combinatorial TF code that controls EE cell diversity, with class-specific TFs Mirr and Ptx1that respectively define two major classes of EEs, and regional TFs such as Esg, Drm, Exex and Fer1that further define regional EE identity. Our single-cell data should greatly facilitate Drosophila modeling of EE differentiation and function.

Keywords: Enteroendocrine Cells, Subtypes, Drosophila, Midgut, Neuropeptide, Transcription Factor Code, Single Cell RNA sequencing, Mirr, Ptx1, Notch

Suggested Citation

Guo, Xingting and Yin, Chang and Yang, Fu and Zhang, Yongchao and Huang, Huanwei and Wang, Jiawen and Deng, Bowen and Cai, Tao and Rao, Yi and Xi, Rongwen, The Cellular Diversity and Transcription Factor Code of Drosophila Enteroendocrine Cells (June 25, 2019). Available at SSRN: https://ssrn.com/abstract=3409458 or http://dx.doi.org/10.2139/ssrn.3409458
This is a paper under consideration at Cell Press and has not been peer-reviewed.

Xingting Guo

Tsinghua University - National Institute of Biological Sciences

7 Science Park Road
ZGC Life Science Park
Beijing, 102206
China

Chang Yin

Tsinghua University - National Institute of Biological Sciences

7 Science Park Road
ZGC Life Science Park
Beijing, 102206
China

Fu Yang

Tsinghua University - National Institute of Biological Sciences

7 Science Park Road
ZGC Life Science Park
Beijing, 102206
China

Yongchao Zhang

Tsinghua University - National Institute of Biological Sciences

7 Science Park Road
ZGC Life Science Park
Beijing, 102206
China

Huanwei Huang

Tsinghua University - National Institute of Biological Sciences

7 Science Park Road
ZGC Life Science Park
Beijing, 102206
China

Jiawen Wang

Tsinghua University - National Institute of Biological Sciences

7 Science Park Road
ZGC Life Science Park
Beijing, 102206
China

Bowen Deng

Peking University - School of Life Science

Beijing
China

Tao Cai

Tsinghua University - National Institute of Biological Sciences

7 Science Park Road
ZGC Life Science Park
Beijing, 102206
China

Yi Rao

Peking University - School of Life Science

Beijing
China

Rongwen Xi (Contact Author)

Tsinghua University - National Institute of Biological Sciences

7 Science Park Road
ZGC Life Science Park
Beijing, 102206
China

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