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Ocular Surface Repair Using Decellularized Porcine Conjunctiva

43 Pages Posted: 8 Jul 2019 First Look: Accepted

See all articles by Long Zhao

Long Zhao

Shandong First Medical University - State Key Laboratory Cultivation Base

Yanni Jia

Shandong First Medical University - Shandong Eye Hospital

Can Zhao

Shandong First Medical University - Shandong Eye Hospital

Ting Liu

Shandong First Medical University - State Key Laboratory Cultivation Base

Songmei Zhang

Shandong First Medical University - State Key Laboratory Cultivation Base

Muchen Dong

Shandong First Medical University - Shandong Eye Hospital

Fuyan Wang

Shandong University - Department of Ophthalmology

Hua Li

Shandong University - Department of Ophthalmology

Qingjun Zhou

Shandong First Medical University - State Key Laboratory Cultivation Base

Weiyun Shi

Shandong First Medical University - State Key Laboratory Cultivation Base

Abstract

The primary functions of the conjunctiva embody ocular surface protection and the maintenance of the tear film equilibrium. Severe conjunctival defects such as symblepharon may impair the integrity of ocular surface and cause loss of visual functions. Here we report the use of a decellularized porcine conjunctiva (DPC) for conjunctival reconstruction in rabbit models and in clinic. Our results show that the major xenoantigens are efficiently removed, while abundant matrix components and integrated microstructures are well preserved in the DPC. These characteristics provide mechanical support and favorable histocompatibility for repairing damaged conjunctiva. The DPC application has demonstrated enhanced transplant stability and improved epithelial regeneration in sever ocular surface damage comparing to those of amniotic membrane (AM), the most frequently applied matrix for ocular surface reconstruction nowadays. In order to test the DPC performance in clinic, three patients with pterygium and one patient with symblepharon underwent transplant with DPC. The grafts in all cases were completely re-epithelized and no graft melt or fibroplasia were observed. These results suggest that the strategy we developed is feasible and effective for conjunctival reconstruction and ocular surface repair.

Keywords: Decellularization, conjunctiva, symblepharon, conjunctival reconstruction, ocular surface repair

Suggested Citation

Zhao, Long and Jia, Yanni and Zhao, Can and Liu, Ting and Zhang, Songmei and Dong, Muchen and Wang, Fuyan and Li, Hua and Zhou, Qingjun and Shi, Weiyun, Ocular Surface Repair Using Decellularized Porcine Conjunctiva (July 5, 2019). Available at SSRN: https://ssrn.com/abstract=3415230 or http://dx.doi.org/10.2139/ssrn.3415230

Long Zhao

Shandong First Medical University - State Key Laboratory Cultivation Base

China

Yanni Jia

Shandong First Medical University - Shandong Eye Hospital

China

Can Zhao

Shandong First Medical University - Shandong Eye Hospital

China

Ting Liu

Shandong First Medical University - State Key Laboratory Cultivation Base

China

Songmei Zhang

Shandong First Medical University - State Key Laboratory Cultivation Base

China

Muchen Dong

Shandong First Medical University - Shandong Eye Hospital

China

Fuyan Wang

Shandong University - Department of Ophthalmology

China

Hua Li

Shandong University - Department of Ophthalmology

China

Qingjun Zhou

Shandong First Medical University - State Key Laboratory Cultivation Base ( email )

China

Weiyun Shi (Contact Author)

Shandong First Medical University - State Key Laboratory Cultivation Base ( email )

China

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