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Biofunctionalized Chondrogenic Shape-Memory Ternary Scaffolds for Efficient Cell-Free Cartilage Regeneration

41 Pages Posted: 23 Jul 2019 Publication Status: Accepted

See all articles by Huixia Xuan

Huixia Xuan

Donghua University - State Key Laboratory for Modification of Chemical Fibers and Polymer Materials; Donghua University - International Joint Laboratory for Advanced Fiber and Low-dimension Materials

Haoran Hu

Shanghai Jiao Tong University (SJTU) - Department of Orthopedics

Congying Geng

Donghua University - State Key Laboratory for Modification of Chemical Fibers and Polymer Materials; Donghua University - International Joint Laboratory for Advanced Fiber and Low-dimension Materials

Jianchun Song

Donghua University - State Key Laboratory for Modification of Chemical Fibers and Polymer Materials; Donghua University - International Joint Laboratory for Advanced Fiber and Low-dimension Materials

Yifan Shen

Shanghai Jiao Tong University (SJTU) - Department of Orthopedics

Dong Lei

Donghua University - State Key Laboratory for Modification of Chemical Fibers and Polymer Materials; Donghua University - International Joint Laboratory for Advanced Fiber and Low-dimension Materials

Qingbao Guan

Donghua University - State Key Laboratory for Modification of Chemical Fibers and Polymer Materials; Donghua University - International Joint Laboratory for Advanced Fiber and Low-dimension Materials

Shichang Zhao

Shanghai Jiao Tong University (SJTU) - Department of Orthopedics

Zhengwei You

Donghua University - College of Chemistry, Chemical Engineering & Biotechnology; Donghua University - State Key Laboratory for Modification of Chemical Fibers and Polymer Materials

Abstract

Cartilage defect repair remains a great clinical challenge due to the limited self-regeneration capacity of cartilage tissue. Surgical treatment of injured cartilage is rather difficult due to the narrow space in the articular cavity and irregular defect area. Herein, we designed and fabricated chondrogenic and physiological-temperature-triggered shape-memory ternary scaffolds for cell-free cartilage repair, where the poly (glycerol sebacate) (PGS) networks ensured good elasticity and shape recovery, crystallized poly (1,3-propylene sebacate) (PPS) acted as switchable phase, and immobilized bioactive kartogenin (KGN) endowed the scaffold with chondrogenic capacity. The resultant scaffolds exhibited excellent shape-memory properties with shape-memory fixed ratio of 97.6% and recovered ratio of 96.8% at 37 °C for PPS/PGS/KGN-100, indicating a good potential for minimally invasive implantation. The scaffolds gradually degraded in Dulbecco's phosphate-buffered saline and released KGN up to 12 weeks in vitro. In addition, the scaffolds promoted chondrogenic differentiation while inhibiting osteogenic differentiation of bone marrow-derived mesenchymal stem cells in a concentration-dependent manner. Consequently, the PPS/PGS/KGN-100 scaffolds efficiently stimulated the formation of an overlying layer of hyaline cartilage mimicking the characteristic architecture of native articular cartilage even in the absence of exogenous growth factors and seeded cells. This study provides much inspiration for future research on cartilage tissue engineering.

Keywords: Shape-memory scaffold, chondrogenic, cell-free cartilage regeneration, minimally invasive implantation, kartogenin

Suggested Citation

Xuan, Huixia and Hu, Haoran and Geng, Congying and Song, Jianchun and Shen, Yifan and Lei, Dong and Guan, Qingbao and Zhao, Shichang and You, Zhengwei, Biofunctionalized Chondrogenic Shape-Memory Ternary Scaffolds for Efficient Cell-Free Cartilage Regeneration (2019). Available at SSRN: https://ssrn.com/abstract=3423491 or http://dx.doi.org/10.2139/ssrn.3423491

Huixia Xuan

Donghua University - State Key Laboratory for Modification of Chemical Fibers and Polymer Materials

Shanghai
China

Donghua University - International Joint Laboratory for Advanced Fiber and Low-dimension Materials

Shanghai
China

Haoran Hu

Shanghai Jiao Tong University (SJTU) - Department of Orthopedics

China

Congying Geng

Donghua University - State Key Laboratory for Modification of Chemical Fibers and Polymer Materials

Shanghai
China

Donghua University - International Joint Laboratory for Advanced Fiber and Low-dimension Materials

Shanghai
China

Jianchun Song

Donghua University - State Key Laboratory for Modification of Chemical Fibers and Polymer Materials

Shanghai
China

Donghua University - International Joint Laboratory for Advanced Fiber and Low-dimension Materials

Shanghai
China

Yifan Shen

Shanghai Jiao Tong University (SJTU) - Department of Orthopedics

China

Dong Lei

Donghua University - State Key Laboratory for Modification of Chemical Fibers and Polymer Materials

Shanghai
China

Donghua University - International Joint Laboratory for Advanced Fiber and Low-dimension Materials

Shanghai
China

Qingbao Guan

Donghua University - State Key Laboratory for Modification of Chemical Fibers and Polymer Materials

Shanghai
China

Donghua University - International Joint Laboratory for Advanced Fiber and Low-dimension Materials

Shanghai
China

Shichang Zhao

Shanghai Jiao Tong University (SJTU) - Department of Orthopedics ( email )

China

Zhengwei You (Contact Author)

Donghua University - College of Chemistry, Chemical Engineering & Biotechnology ( email )

Shanghai
China

Donghua University - State Key Laboratory for Modification of Chemical Fibers and Polymer Materials ( email )

Shanghai
China

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